Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K6139_RS00550 Genome accession   NZ_CP082053
Coordinates   118221..118661 (-) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain MS20677     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 113221..123661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6139_RS00525 (K6139_00515) coaE 113711..114331 (-) 621 WP_001269524.1 dephospho-CoA kinase -
  K6139_RS00530 (K6139_00520) - 114356..114400 (+) 45 WP_120795372.1 protein YacM -
  K6139_RS00535 (K6139_00525) guaC 114556..115599 (+) 1044 WP_001217338.1 GMP reductase -
  K6139_RS00540 (K6139_00530) hofC 115634..116836 (-) 1203 WP_001540700.1 protein transport protein HofC -
  K6139_RS00545 (K6139_00535) pilB 116826..118211 (-) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  K6139_RS00550 (K6139_00540) pilA 118221..118661 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  K6139_RS00555 (K6139_00545) nadC 118865..119758 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K6139_RS00560 (K6139_00550) ampD 119846..120397 (+) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6139_RS00565 (K6139_00555) ampE 120394..121248 (+) 855 WP_000172010.1 beta-lactamase regulator AmpE -
  K6139_RS00570 (K6139_00560) aroP 121291..122661 (-) 1371 WP_001540704.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=524913 K6139_RS00550 WP_000360914.1 118221..118661(-) (pilA) [Escherichia coli strain MS20677]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=524913 K6139_RS00550 WP_000360914.1 118221..118661(-) (pilA) [Escherichia coli strain MS20677]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363