Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   K6131_RS17525 Genome accession   NZ_CP082047
Coordinates   3580560..3581237 (+) Length   225 a.a.
NCBI ID   WP_000178175.1    Uniprot ID   A0A0H2Z3H2
Organism   Escherichia coli strain MS21199     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3575560..3586237
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6131_RS17490 (K6131_17365) gspG 3576040..3576477 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  K6131_RS17495 (K6131_17370) gspH 3576485..3576994 (+) 510 WP_001539909.1 type II secretion system minor pseudopilin GspH -
  K6131_RS17500 (K6131_17375) gspI 3576991..3577368 (+) 378 WP_001041748.1 type II secretion system minor pseudopilin GspI -
  K6131_RS17505 (K6131_17380) gspJ 3577361..3577948 (+) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  K6131_RS17510 (K6131_17385) gspK 3577941..3578924 (+) 984 WP_001058513.1 type II secretion system minor pseudopilin GspK -
  K6131_RS17515 (K6131_17390) gspL 3578939..3580102 (+) 1164 Protein_3429 type II secretion system protein GspL -
  K6131_RS17520 (K6131_17395) gspM 3580099..3580560 (+) 462 WP_001298207.1 type II secretion system protein GspM -
  K6131_RS17525 (K6131_17400) pilD 3580560..3581237 (+) 678 WP_000178175.1 prepilin peptidase GspO Machinery gene
  K6131_RS17530 (K6131_17405) bfr 3581266..3581742 (-) 477 WP_000675504.1 bacterioferritin -
  K6131_RS17535 (K6131_17410) bfd 3581814..3582008 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  K6131_RS17540 (K6131_17415) chiA 3582177..3584879 (-) 2703 WP_000773146.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24870.75 Da        Isoelectric Point: 7.6364

>NTDB_id=524874 K6131_RS17525 WP_000178175.1 3580560..3581237(+) (pilD) [Escherichia coli strain MS21199]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=524874 K6131_RS17525 WP_000178175.1 3580560..3581237(+) (pilD) [Escherichia coli strain MS21199]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387