Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   JCR32_RS20450 Genome accession   NZ_CP067069
Coordinates   3979761..3980507 (-) Length   248 a.a.
NCBI ID   WP_000487010.1    Uniprot ID   Q81LT9
Organism   Bacillus sp. HNR-4     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 3974761..3985507
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JCR32_RS20430 (JCR32_20330) dnaG 3975531..3977327 (-) 1797 WP_062923548.1 DNA primase -
  JCR32_RS20435 (JCR32_20335) - 3978000..3978143 (-) 144 WP_154644226.1 hypothetical protein -
  JCR32_RS20440 (JCR32_20340) - 3978179..3978991 (-) 813 WP_000368943.1 pyruvate, water dikinase regulatory protein -
  JCR32_RS20445 (JCR32_20345) - 3979021..3979653 (-) 633 WP_000583759.1 helix-turn-helix transcriptional regulator -
  JCR32_RS20450 (JCR32_20350) recO 3979761..3980507 (-) 747 WP_000487010.1 DNA repair protein RecO Machinery gene
  JCR32_RS20455 (JCR32_20355) - 3980541..3980684 (-) 144 WP_000883924.1 YqzL family protein -
  JCR32_RS20460 (JCR32_20360) era 3980861..3981766 (-) 906 WP_001080241.1 GTPase Era -
  JCR32_RS20465 (JCR32_20365) - 3981759..3982157 (-) 399 WP_001086294.1 cytidine deaminase -
  JCR32_RS20470 (JCR32_20370) - 3982257..3982610 (-) 354 WP_000715479.1 diacylglycerol kinase family protein -
  JCR32_RS20475 (JCR32_20375) ybeY 3982607..3983077 (-) 471 WP_000054692.1 rRNA maturation RNase YbeY -
  JCR32_RS20480 (JCR32_20380) - 3983074..3985218 (-) 2145 WP_000946454.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28665.22 Da        Isoelectric Point: 8.0540

>NTDB_id=524817 JCR32_RS20450 WP_000487010.1 3979761..3980507(-) (recO) [Bacillus sp. HNR-4]
MFQKVEGIVIRTTDYGETNKIVTIFSRELGKVSAMARGAKKPKSRLASVSQLMTHGHFLIQMGSGLGTLQQGEIISTMKE
IREDIFLTAYASFIVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDQYRIPVGEAVHKLLRLFYHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=524817 JCR32_RS20450 WP_000487010.1 3979761..3980507(-) (recO) [Bacillus sp. HNR-4]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAACAAGATTGTTACAATATTTTCAAG
AGAATTGGGTAAGGTAAGCGCAATGGCAAGAGGAGCGAAAAAACCGAAAAGCCGGTTAGCATCTGTTTCGCAACTTATGA
CACATGGTCATTTTCTTATTCAAATGGGATCTGGACTTGGAACTTTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTGTTGAATTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCATATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATCCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCGTACTTTGACACATGTGCGATTTGTCATCAAGAAACAGAT
TTTGTCGCCTTCTCTGTTAGAGAGGGCGGTTTTCTGTGCTCACGTCATGCTGAGCAAGATCAGTACCGTATACCAGTGGG
GGAAGCTGTTCATAAATTATTGCGTCTTTTCTACCACTTCGATTTACATAGGCTTGGCAATGTATCAGTGAAGGATAGCA
CAAAAAAACAAATGCGTTTAGTATTGAATACATATTATGATGAATATTGCGGGATTTATTTGAAATCAAGACGTTTCTTA
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-238)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q81LT9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552