Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   JCR32_RS20175 Genome accession   NZ_CP067069
Coordinates   3927166..3928209 (-) Length   347 a.a.
NCBI ID   WP_001013200.1    Uniprot ID   A0AA96SWA8
Organism   Bacillus sp. HNR-4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3922166..3933209
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JCR32_RS20130 (JCR32_20030) - 3922594..3922794 (-) 201 WP_000106079.1 YqzE family protein -
  JCR32_RS20135 (JCR32_20035) aroK 3922833..3923330 (-) 498 WP_076870186.1 shikimate kinase AroK -
  JCR32_RS20140 (JCR32_20040) - 3923450..3924100 (-) 651 WP_274430258.1 2OG-Fe(II) oxygenase -
  JCR32_RS20145 (JCR32_20045) comGG 3924276..3924647 (-) 372 WP_274430259.1 competence type IV pilus minor pilin ComGG -
  JCR32_RS20150 (JCR32_20050) comGF 3924644..3925114 (-) 471 WP_274431563.1 competence type IV pilus minor pilin ComGF -
  JCR32_RS20155 (JCR32_20055) comGE 3925084..3925386 (-) 303 WP_274431564.1 competence type IV pilus minor pilin ComGE -
  JCR32_RS20160 (JCR32_20060) comGD 3925379..3925834 (-) 456 WP_076870182.1 comG operon protein ComGD -
  JCR32_RS20165 (JCR32_20065) comGC 3925831..3926130 (-) 300 WP_001178696.1 comG operon protein ComGC -
  JCR32_RS20170 (JCR32_20070) comGB 3926142..3927173 (-) 1032 WP_076870181.1 comG operon protein ComGB -
  JCR32_RS20175 (JCR32_20075) comGA 3927166..3928209 (-) 1044 WP_001013200.1 competence protein ComGA Machinery gene
  JCR32_RS20180 (JCR32_20080) - 3928415..3929110 (+) 696 WP_274430260.1 helix-turn-helix domain-containing protein -
  JCR32_RS20185 (JCR32_20085) - 3929236..3929478 (+) 243 WP_000440711.1 DUF2626 domain-containing protein -
  JCR32_RS20190 (JCR32_20090) - 3929586..3930980 (+) 1395 WP_001094329.1 L-cystine transporter -
  JCR32_RS20195 (JCR32_20095) - 3931156..3931563 (-) 408 WP_274430261.1 hypothetical protein -
  JCR32_RS20200 (JCR32_20100) - 3931651..3931866 (-) 216 WP_001008320.1 DUF3912 family protein -
  JCR32_RS20205 (JCR32_20105) - 3932119..3932430 (+) 312 WP_001093243.1 hypothetical protein -
  JCR32_RS20210 (JCR32_20110) - 3932467..3932955 (-) 489 WP_000764252.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39412.97 Da        Isoelectric Point: 9.2147

>NTDB_id=524816 JCR32_RS20175 WP_001013200.1 3927166..3928209(-) (comGA) [Bacillus sp. HNR-4]
MNGIESFANMILKEACRVQASDLHIVPRQKDVVVQLRIGKDLMTKYCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKKLLSFLRYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRNDDVLQIQINEKAGITYEAGLKAILRHDPDVILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDARGAILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCKRKCSTLCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLQSSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=524816 JCR32_RS20175 WP_001013200.1 3927166..3928209(-) (comGA) [Bacillus sp. HNR-4]
ATGAATGGAATTGAAAGCTTTGCGAATATGATTTTGAAAGAAGCGTGCAGGGTACAAGCGTCGGACTTACATATTGTGCC
CCGGCAGAAGGATGTAGTGGTTCAGCTGCGTATAGGAAAAGATTTAATGACGAAATATTGTATTGAAAAGGAATTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCTATGGATATAGGGGAGAGGCGGAAGCCACAAAATGGTTCACTGTAT
TTACAAATGGATGGACAAGAAGTGTATTTGCGTCTTTCCACGCTTCCAACCGTATACCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAGAAACTACTTTCTTTTTTAC
GTTATTCTCATGGATTACTCGTATTTACTGGACCGACTGGTTCGGGGAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCGTTACACTGGAAGATCCAGTTGAAAAAAGAAATGACGATGTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGACTAAAGGCTATTTTGCGTCATGATCCAGATGTTATTTTAGTCG
GTGAAATTCGTGATGAAGAAACAGCGAAAATTGCTATAAGAGCAAGTTTGACTGGCCATTTAGTAATGACGACATTGCAT
ACGAATGATGCGAGAGGGGCGATACTTCGGTTCATGGATTTTGGCATAACGAGGCAAGAAATCGAACAATCTTTATTAGC
TATAGCTGCACAGCGACTTGTCGAATTAAAGTGTCCGTTTTGCAAAAGAAAGTGCTCAACTTTATGCAAATCAATGAGGC
AAGTAAGGCAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACATACAAGCACGAAACATTACAATCTTCGATACGAAAAGGATACGCTTTAGGGTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.925

100

0.579
  pilB Vibrio campbellii strain DS40M4

35.977

100

0.366