Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   JGE02_RS10990 Genome accession   NZ_CP067042
Coordinates   2338642..2340006 (-) Length   454 a.a.
NCBI ID   WP_005686111.1    Uniprot ID   A0A2A5L5Z5
Organism   Lacticaseibacillus rhamnosus strain B6     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2333642..2345006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JGE02_RS10970 (JGE02_10945) - 2334711..2335469 (-) 759 WP_005692337.1 ABC transporter permease -
  JGE02_RS10975 (JGE02_10950) - 2335471..2336376 (-) 906 WP_005692336.1 ATP-binding cassette domain-containing protein -
  JGE02_RS10980 (JGE02_10955) - 2336518..2337414 (+) 897 WP_005692335.1 helix-turn-helix domain-containing protein -
  JGE02_RS10985 (JGE02_10960) - 2337506..2338621 (-) 1116 WP_005686113.1 PIN/TRAM domain-containing protein -
  JGE02_RS10990 (JGE02_10965) radA 2338642..2340006 (-) 1365 WP_005686111.1 DNA repair protein RadA Machinery gene
  JGE02_RS10995 (JGE02_10970) - 2340026..2340565 (-) 540 WP_005713603.1 dUTP diphosphatase -
  JGE02_RS11000 (JGE02_10975) - 2341210..2341500 (+) 291 WP_005714535.1 GNAT family N-acetyltransferase -
  JGE02_RS11005 (JGE02_10980) - 2341873..2342469 (+) 597 WP_005713617.1 IS607 family transposase -
  JGE02_RS11010 (JGE02_10985) - 2342504..2343685 (+) 1182 WP_005714520.1 RNA-guided endonuclease InsQ/TnpB family protein -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49157.58 Da        Isoelectric Point: 8.3128

>NTDB_id=524594 JGE02_RS10990 WP_005686111.1 2338642..2340006(-) (radA) [Lacticaseibacillus rhamnosus strain B6]
MAKAKTQYVCQNCGYISATYLGRCPNCGGWNTLVEETVSSTKSAPRQTRTGSKVKPTRMDEVTVTKETRVKTGLAELNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSGQLASTGGTVLYVSGEESASQIKMRAGRLGVANSGMYLYPETDMPSIENVI
DQMQPDYVVIDSVQTMNVPDMNSAVGSVAQIREVTAELMRIAKSKGVTIFIVGHVTKEGAIAGPKILEHMVDTVLYFEGD
MHHTYRILRSVKNRFGSTNEIGIFEMHQNGLQEVANPSEIFLEERLAGATGSAVVVSMEGTRPILVEIQALISPTMYGNA
KRTSSGLDHNRVSLIMAVLEKRANLMLQNQDAYLKATGGVKLDEPAIDLAMAVAIASSYRDKEIPPTDCFVGEIGLTGEV
RRVNRIEDRVKEAAKLGFKRIFVPRNNLQGWRAPKDIQVIGVTSIAEALHKVFN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=524594 JGE02_RS10990 WP_005686111.1 2338642..2340006(-) (radA) [Lacticaseibacillus rhamnosus strain B6]
ATGGCGAAAGCAAAAACACAATATGTATGTCAAAATTGCGGTTATATTTCGGCGACTTATTTAGGCCGTTGTCCAAACTG
CGGCGGGTGGAATACGCTCGTCGAAGAAACCGTTAGTTCCACTAAATCGGCGCCGCGACAAACGCGCACCGGCAGCAAGG
TTAAGCCCACACGCATGGACGAGGTCACGGTGACCAAAGAAACCCGGGTTAAAACAGGCTTGGCCGAGTTGAATCGCGTG
CTTGGTGGCGGTGTGGTTCCGGGCTCGTTGGTTCTGATTGGCGGTGATCCCGGGATTGGCAAATCAACCTTGCTGCTGCA
GGTGTCTGGTCAGCTAGCTAGCACAGGTGGTACGGTGTTGTACGTTTCAGGTGAAGAAAGCGCCAGTCAGATTAAAATGC
GGGCAGGGCGGCTAGGTGTTGCCAACTCCGGCATGTATTTATATCCCGAAACCGACATGCCCAGCATCGAAAATGTGATT
GACCAGATGCAACCGGATTATGTGGTGATTGACTCGGTTCAAACCATGAATGTACCGGACATGAATTCGGCAGTCGGCTC
GGTTGCCCAGATTCGGGAAGTCACCGCCGAATTGATGCGAATTGCCAAGTCAAAAGGTGTGACGATTTTTATCGTTGGTC
ACGTGACGAAGGAAGGCGCTATTGCCGGGCCCAAGATTCTTGAGCATATGGTGGACACCGTGCTTTATTTTGAGGGCGAT
ATGCATCATACCTATCGCATCTTAAGGTCGGTCAAGAATCGGTTCGGCTCTACTAACGAAATCGGTATTTTCGAAATGCA
TCAAAATGGCTTACAGGAAGTTGCCAATCCCTCGGAAATCTTCCTGGAAGAACGGCTGGCCGGTGCAACCGGCTCGGCGG
TGGTGGTTTCGATGGAAGGTACCCGTCCGATTCTGGTTGAAATCCAGGCCCTCATCAGCCCGACCATGTATGGCAATGCC
AAACGCACGAGCAGCGGCTTGGATCACAACCGCGTGAGCTTGATCATGGCGGTGCTGGAAAAACGTGCCAATTTGATGTT
GCAAAATCAGGACGCTTACTTGAAAGCAACGGGGGGCGTTAAGCTGGATGAGCCGGCGATTGACTTGGCAATGGCAGTGG
CAATTGCCTCGTCATACCGCGACAAGGAAATCCCGCCGACTGATTGTTTTGTCGGTGAAATTGGCTTAACCGGTGAAGTG
CGGCGGGTGAATCGCATTGAAGACCGGGTGAAAGAGGCGGCTAAGTTAGGGTTTAAACGAATTTTTGTGCCGCGAAATAA
CCTGCAAGGCTGGCGTGCGCCTAAAGATATTCAGGTTATTGGCGTCACAAGCATTGCCGAAGCGTTGCATAAGGTTTTTA
ATTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-427)

AAA domain (AAA_25)

(67-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A5L5Z5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

68.433

99.78

0.683
  radA Streptococcus pneumoniae Rx1

68.433

99.78

0.683
  radA Streptococcus pneumoniae D39

68.433

99.78

0.683
  radA Streptococcus pneumoniae R6

68.433

99.78

0.683
  radA Streptococcus pneumoniae TIGR4

68.433

99.78

0.683
  radA Streptococcus mitis SK321

68.212

99.78

0.681
  radA Bacillus subtilis subsp. subtilis str. 168

64.912

100

0.652