Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   JGS08_RS03530 Genome accession   NZ_CP067022
Coordinates   777848..779215 (+) Length   455 a.a.
NCBI ID   WP_003381736.1    Uniprot ID   Q0EDX1
Organism   Pseudomonas cannabina pv. alisalensis strain MAFF 301419     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 772848..784215
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JGS08_RS03500 (JGS08_03500) cydB 773173..774180 (+) 1008 WP_007252480.1 cytochrome d ubiquinol oxidase subunit II -
  JGS08_RS03505 (JGS08_03505) - 774202..774369 (+) 168 WP_007252479.1 DUF2474 domain-containing protein -
  JGS08_RS03510 (JGS08_03510) - 774469..775593 (-) 1125 WP_007252478.1 class I SAM-dependent methyltransferase -
  JGS08_RS03515 (JGS08_03515) - 775723..775956 (-) 234 WP_007252477.1 helix-turn-helix transcriptional regulator -
  JGS08_RS03520 (JGS08_03520) - 776303..777079 (+) 777 WP_007252476.1 ferredoxin--NADP reductase -
  JGS08_RS03525 (JGS08_03525) mscL 777205..777651 (-) 447 WP_007252475.1 large-conductance mechanosensitive channel protein MscL -
  JGS08_RS03530 (JGS08_03530) radA 777848..779215 (+) 1368 WP_003381736.1 DNA repair protein RadA Machinery gene
  JGS08_RS03535 (JGS08_03535) - 779228..779596 (-) 369 WP_007252474.1 PilZ domain-containing protein -
  JGS08_RS03540 (JGS08_03540) - 779880..781955 (+) 2076 WP_042914127.1 carbon starvation CstA family protein -
  JGS08_RS03545 (JGS08_03545) - 781983..782180 (+) 198 WP_002555232.1 YbdD/YjiX family protein -
  JGS08_RS03550 (JGS08_03550) yjiA 782276..783253 (+) 978 WP_007252472.1 GTPase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48839.40 Da        Isoelectric Point: 7.1315

>NTDB_id=524498 JGS08_RS03530 WP_003381736.1 777848..779215(+) (radA) [Pseudomonas cannabina pv. alisalensis strain MAFF 301419]
MAKAKRLYGCTECGATFPKWAGQCADCGAWNTLVETMIESGAAAPPSGRTGWTGSQAQIRTLAEVSVEEIPRFSTNSAEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAQRMPALYVTGEESQQQVAMRARRLGLPQDKLRVMTETCIETIIA
TAKVEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHMS
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPPGLQIIAVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=524498 JGS08_RS03530 WP_003381736.1 777848..779215(+) (radA) [Pseudomonas cannabina pv. alisalensis strain MAFF 301419]
ATGGCCAAGGCCAAACGCTTGTACGGCTGCACCGAGTGCGGCGCGACATTTCCCAAGTGGGCGGGCCAGTGTGCCGATTG
CGGTGCCTGGAACACACTGGTCGAAACCATGATTGAAAGCGGTGCCGCCGCGCCGCCCAGCGGACGTACCGGCTGGACCG
GCTCGCAGGCACAGATCAGAACCCTGGCGGAAGTCAGCGTCGAAGAGATTCCGCGTTTTTCCACCAACTCCGCCGAACTG
GATCGCGTGCTCGGTGGCGGTCTGGTGGACGGCTCGGTGGTGCTGATCGGCGGCGACCCCGGCATCGGCAAGTCGACGAT
TCTGTTGCAGACCCTCTGCAATATCGCCCAGCGCATGCCGGCGCTGTATGTCACTGGCGAAGAATCCCAACAGCAGGTCG
CCATGCGCGCTCGGCGTCTGGGCCTGCCGCAGGACAAGCTGCGCGTCATGACCGAAACCTGCATCGAAACCATCATCGCC
ACGGCCAAGGTCGAGAAGCCCAAGGTGATGGTGATCGACTCGATCCAGACGATCTTCACCGAGCAATTGCAGTCCGCACC
GGGCGGCGTGTCGCAGGTTCGCGAAAGTGCTGCCTTGCTGGTGCGTTACGCGAAACAGAGCGGTACGGCGATCTTTCTGG
TCGGTCACGTCACCAAGGAAGGCGCGCTGGCCGGCCCTCGCGTGCTGGAACACATGGTCGACACTGTGCTGTATTTCGAA
GGCGAGTCGGATGGGCGTCTGCGCTTGCTGCGGGCAGTGAAAAACCGTTTCGGCGCAGTCAACGAACTGGGCGTGTTCGG
CATGACCGACAAGGGCTTGAAGGAAGTCTCCAACCCGTCGGCGATTTTCCTGACCCGCGCGCAGGAAGAAGTACCCGGCA
GCGTGGTCATGGCCACTTGGGAAGGCACGCGTCCGATGCTGGTGGAAGTTCAGGCGCTGGTGGATGACAGCCACATGTCC
AACCCGCGCCGGGTCACCCTGGGGCTGGATCAGAATCGACTGGCGATGCTGCTGGCGGTGTTGCACCGACACGGCGGCAT
TCCGACCCACGATCAGGACGTGTTTCTCAACGTGGTCGGCGGCGTGAAAGTGCTGGAGACTGCATCGGATCTGGCGTTGA
TGGCCGCTGTCATGTCTAGCCTGCGCAATCGCCCGCTGCCGCATGACTTGCTAGTGTTCGGCGAAGTCGGGCTGTCTGGC
GAAGTGCGGCCGGTGCCGAGTGGCCAGGAGCGCCTCAAAGAAGCCGCCAAGCATGGCTTCAAGCGGGCCATCGTGCCTAA
AGGCAACGCGCCGAAAGAAGCGCCTCCCGGCTTGCAGATCATCGCCGTCACGCGGCTGGAGCAGGCACTCGACGCCCTGT
TCGAATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)

AAA domain (AAA_25)

(73-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0EDX1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.123

100

0.492
  radA Streptococcus pneumoniae Rx1

46.739

100

0.473
  radA Streptococcus pneumoniae R6

46.739

100

0.473
  radA Streptococcus pneumoniae TIGR4

46.739

100

0.473
  radA Streptococcus pneumoniae D39

46.739

100

0.473
  radA Streptococcus mitis SK321

46.93

100

0.47
  radA Streptococcus mitis NCTC 12261

46.711

100

0.468