Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   JFY49_RS14260 Genome accession   NZ_CP067019
Coordinates   2894253..2894678 (-) Length   141 a.a.
NCBI ID   WP_166168628.1    Uniprot ID   -
Organism   Acinetobacter sp. CS-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2894678..2916234 2894253..2894678 flank 0


Gene organization within MGE regions


Location: 2894253..2916234
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JFY49_RS14260 (JFY49_14260) comF 2894253..2894678 (-) 426 WP_166168628.1 type IV pilin protein Machinery gene
  JFY49_RS14265 (JFY49_14265) - 2894678..2895271 (-) 594 WP_200223265.1 type IV pilin protein -
  JFY49_RS14270 (JFY49_14270) - 2895280..2899341 (-) 4062 WP_200223266.1 pilus assembly protein -
  JFY49_RS14275 (JFY49_14275) - 2899359..2900045 (-) 687 WP_166168626.1 pilus assembly protein PilX -
  JFY49_RS14280 (JFY49_14280) - 2900042..2900695 (-) 654 Protein_2782 PilW family protein -
  JFY49_RS14285 (JFY49_14285) - 2900712..2901740 (-) 1029 WP_200223267.1 transposase -
  JFY49_RS14290 (JFY49_14290) - 2901796..2902140 (-) 345 Protein_2784 PilW family protein -
  JFY49_RS14295 (JFY49_14295) pilV 2902130..2902615 (-) 486 WP_200223268.1 type IV pilus modification protein PilV -
  JFY49_RS14300 (JFY49_14300) - 2902635..2903066 (-) 432 WP_166168623.1 Tfp pilus assembly protein FimT/FimU -
  JFY49_RS14305 (JFY49_14305) ispH 2903232..2904182 (-) 951 WP_200223269.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  JFY49_RS14310 (JFY49_14310) gmk 2904317..2904940 (+) 624 WP_086197186.1 guanylate kinase -
  JFY49_RS14315 (JFY49_14315) rpoZ 2905017..2905298 (+) 282 WP_004896248.1 DNA-directed RNA polymerase subunit omega -
  JFY49_RS14320 (JFY49_14320) - 2905518..2907623 (+) 2106 WP_200223270.1 RelA/SpoT family protein -
  JFY49_RS14325 (JFY49_14325) - 2907693..2908076 (+) 384 WP_092820181.1 RidA family protein -
  JFY49_RS14330 (JFY49_14330) - 2908299..2908493 (+) 195 WP_086174534.1 bacterioferritin-associated ferredoxin -
  JFY49_RS14335 (JFY49_14335) bfr 2908747..2909211 (+) 465 WP_092820182.1 heteropolymeric bacterioferritin subunit Bfr -
  JFY49_RS14340 (JFY49_14340) - 2909273..2910907 (-) 1635 WP_200223271.1 PglL family O-oligosaccharyltransferase -
  JFY49_RS14345 (JFY49_14345) - 2911083..2911301 (-) 219 WP_227609476.1 hypothetical protein -
  JFY49_RS14355 (JFY49_14355) - 2912236..2913546 (-) 1311 WP_200223274.1 O-antigen ligase family protein -
  JFY49_RS14360 (JFY49_14360) - 2913545..2913727 (+) 183 WP_200223275.1 hypothetical protein -
  JFY49_RS14365 (JFY49_14365) comP 2913840..2914286 (-) 447 WP_200223276.1 pilin Machinery gene
  JFY49_RS14375 (JFY49_14375) - 2915674..2916234 (-) 561 WP_200223277.1 TPM domain-containing protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15446.52 Da        Isoelectric Point: 8.9025

>NTDB_id=524482 JFY49_RS14260 WP_166168628.1 2894253..2894678(-) (comF) [Acinetobacter sp. CS-2]
MNKNKGFTLIELMIVVAIIAVLAAIAYPSYTQYKIRTNRADVQSEMMNIAQRLQSYYVINHNYTSASLDNGTTSKDYPIS
NPVYRINLVPNGQSYTLTATPISTSSQAGDGIICLNNQGQKFWAKGATACVISATSTWDGH

Nucleotide


Download         Length: 426 bp        

>NTDB_id=524482 JFY49_RS14260 WP_166168628.1 2894253..2894678(-) (comF) [Acinetobacter sp. CS-2]
ATGAATAAAAATAAAGGATTTACCCTAATCGAGTTAATGATTGTCGTAGCAATAATTGCTGTTTTGGCAGCGATTGCTTA
CCCATCTTATACTCAATATAAAATTCGTACTAACCGTGCTGATGTACAAAGTGAAATGATGAATATTGCACAGCGCTTAC
AAAGTTATTACGTGATCAATCATAATTATACGAGCGCAAGCTTAGATAATGGAACGACCAGCAAGGACTATCCAATATCT
AATCCTGTGTATAGAATTAACCTTGTTCCGAATGGTCAAAGCTATACATTAACTGCAACCCCCATATCCACTTCATCACA
AGCAGGAGATGGTATTATTTGTTTAAATAATCAAGGACAAAAATTCTGGGCCAAAGGTGCAACAGCTTGTGTAATTTCAG
CAACTTCTACTTGGGATGGTCACTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(28-114)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

59.028

100

0.603
  pilE Acinetobacter baumannii D1279779

57.353

96.454

0.553