Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   JFY49_RS02615 Genome accession   NZ_CP067019
Coordinates   569418..570179 (+) Length   253 a.a.
NCBI ID   WP_180044364.1    Uniprot ID   -
Organism   Acinetobacter sp. CS-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 564418..575179
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JFY49_RS02585 (JFY49_02585) - 564686..565099 (+) 414 WP_180044625.1 hypothetical protein -
  JFY49_RS02590 (JFY49_02590) - 565304..566584 (+) 1281 WP_200223610.1 glycosyltransferase family 4 protein -
  JFY49_RS02595 (JFY49_02595) - 566616..567167 (+) 552 WP_200223611.1 phosphatase PAP2 family protein -
  JFY49_RS02600 (JFY49_02600) iscX 567256..567456 (+) 201 WP_092819025.1 Fe-S cluster assembly protein IscX -
  JFY49_RS02605 (JFY49_02605) ndk 567568..567999 (+) 432 WP_086195365.1 nucleoside-diphosphate kinase -
  JFY49_RS02610 (JFY49_02610) rlmN 568122..569357 (+) 1236 WP_200223612.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  JFY49_RS02615 (JFY49_02615) pilF 569418..570179 (+) 762 WP_180044364.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  JFY49_RS02620 (JFY49_02620) - 570170..570961 (+) 792 WP_200223613.1 helix-turn-helix domain-containing protein -
  JFY49_RS02625 (JFY49_02625) ispG 570986..572107 (+) 1122 WP_086195369.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  JFY49_RS02630 (JFY49_02630) hisS 572111..573406 (+) 1296 WP_200223615.1 histidine--tRNA ligase -
  JFY49_RS02635 (JFY49_02635) - 573436..574137 (+) 702 WP_200223616.1 YfgM family protein -

Sequence

Protein


Download         Length: 253 a.a.        Molecular weight: 28572.37 Da        Isoelectric Point: 9.2227

>NTDB_id=524464 JFY49_RS02615 WP_180044364.1 569418..570179(+) (pilF) [Acinetobacter sp. CS-2]
MSAIFVAGCQTPATIGKKDPEKAVKVRTQLAAEYIKSGDLDSAKRTLDQALEVDSRDSTANMMMGVLLQQEGSKISMDKA
ESYFKRAISADPKNAQARNNYGTYLYQIERYNDALEQLNVAGTTLGYEQRYRALENAGRIYLKQGDMVNAEKTFKQALQV
NRDSYVSMLELSEIFYLKQQTAAATQLYEQFVRSVGQKNQGARALWIGIRIARANSDPMGTQVLVNQLRALFPESPEYQR
YLQLQYSTEAVWK

Nucleotide


Download         Length: 762 bp        

>NTDB_id=524464 JFY49_RS02615 WP_180044364.1 569418..570179(+) (pilF) [Acinetobacter sp. CS-2]
ATGTCCGCGATTTTTGTTGCCGGGTGTCAGACACCAGCAACAATCGGGAAAAAAGATCCTGAGAAGGCAGTGAAAGTTCG
CACTCAATTGGCGGCAGAATATATTAAGTCGGGTGACCTGGATTCAGCAAAACGTACTTTGGATCAGGCACTGGAAGTAG
ATTCACGTGATTCAACAGCAAATATGATGATGGGGGTTTTACTGCAACAAGAGGGCAGTAAAATCAGCATGGACAAAGCA
GAGAGCTATTTTAAGCGCGCAATTTCTGCTGATCCGAAAAATGCACAAGCGCGTAACAACTACGGTACTTATTTGTATCA
AATTGAGCGTTATAATGACGCGCTTGAACAACTCAATGTGGCCGGAACTACGTTGGGCTATGAACAACGCTACAGAGCAT
TGGAAAATGCGGGACGGATTTATTTAAAGCAAGGCGATATGGTGAATGCTGAAAAAACATTCAAACAGGCTCTGCAAGTA
AATCGTGACTCTTATGTTTCAATGTTAGAGTTGTCAGAAATTTTTTATCTCAAACAGCAGACCGCCGCTGCAACACAATT
GTATGAACAGTTTGTGCGCAGTGTCGGTCAAAAGAATCAAGGTGCGCGTGCGCTTTGGATTGGTATCCGAATTGCGCGGG
CAAATAGCGACCCAATGGGAACACAGGTGCTTGTCAATCAGTTGCGCGCGCTTTTCCCCGAGAGTCCGGAATACCAACGT
TATCTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(26-55)

Tetratricopeptide repeat (TPR_19)

(77-134)

Tetratricopeptide repeat (TPR_16)

(136-192)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

80.478

99.209

0.798
  pilF Acinetobacter baylyi ADP1

71.542

100

0.715