Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K6T45_RS05990 Genome accession   NZ_CP081927
Coordinates   1284493..1285449 (+) Length   318 a.a.
NCBI ID   WP_004916143.1    Uniprot ID   E4TAL4
Organism   Riemerella anatipestifer strain 20160930R-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1279493..1290449
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6T45_RS09800 - 1279967..1280311 (+) 345 WP_233406961.1 hypothetical protein -
  K6T45_RS09805 - 1280326..1280610 (+) 285 WP_260283626.1 DUF4271 domain-containing protein -
  K6T45_RS05965 (K6T45_05965) - 1280633..1281376 (+) 744 WP_013447024.1 uroporphyrinogen-III synthase -
  K6T45_RS05970 (K6T45_05970) - 1281468..1283516 (+) 2049 WP_004916139.1 S9 family peptidase -
  K6T45_RS05975 (K6T45_05975) rpmB 1283791..1284027 (+) 237 WP_004916140.1 50S ribosomal protein L28 -
  K6T45_RS05980 (K6T45_05980) rpmG 1284038..1284220 (+) 183 WP_004916141.1 50S ribosomal protein L33 -
  K6T45_RS05985 (K6T45_05985) - 1284243..1284392 (+) 150 WP_004916142.1 DUF4295 family protein -
  K6T45_RS05990 (K6T45_05990) pilA 1284493..1285449 (+) 957 WP_004916143.1 signal recognition particle-docking protein FtsY Machinery gene
  K6T45_RS05995 (K6T45_05995) - 1285490..1285744 (+) 255 WP_004916144.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  K6T45_RS06000 (K6T45_06000) topA 1285931..1288450 (+) 2520 WP_004916145.1 type I DNA topoisomerase -
  K6T45_RS06005 (K6T45_06005) - 1288462..1289526 (+) 1065 WP_004916146.1 formimidoylglutamase -

Sequence

Protein


Download         Length: 318 a.a.        Molecular weight: 34733.97 Da        Isoelectric Point: 6.0732

>NTDB_id=524432 K6T45_RS05990 WP_004916143.1 1284493..1285449(+) (pilA) [Riemerella anatipestifer strain 20160930R-3]
MSWFKKIFKKEEKETLDRGLEKSSQGFFEKISKAVIGKSKVDDEVLDDLEEVLIASDVGAQTTIKIIERIEDRVARDKYV
GVDELHKLLKEEITALLLENPHADTGNIDEAKKPYVIMVVGVNGVGKTTTIGKLAHQFKTQGKKVVLGAADTFRAAAVDQ
LVIWSERVGVPIVKQNMGSDPASVAFDTVQSAVAQNADVVLIDTAGRLHNKVNLMNELSKIKRVMQKVIPDAPHEVLLVL
DGSTGQNAFEQAKQFTAATEVNALAVTKLDGTAKGGVVIGISDQFQVPVKYIGVGEKMEDLQLFNGTEFVDSLFKKKS

Nucleotide


Download         Length: 957 bp        

>NTDB_id=524432 K6T45_RS05990 WP_004916143.1 1284493..1285449(+) (pilA) [Riemerella anatipestifer strain 20160930R-3]
ATGAGTTGGTTTAAAAAAATATTCAAAAAAGAAGAGAAGGAAACTTTAGATAGAGGGTTAGAAAAGTCAAGTCAAGGCTT
TTTTGAAAAAATATCCAAAGCAGTCATAGGGAAAAGTAAGGTAGATGATGAAGTTTTAGATGATTTGGAAGAGGTTCTTA
TTGCATCAGATGTAGGGGCTCAGACAACTATCAAGATTATCGAACGAATTGAAGATAGAGTTGCTAGGGATAAGTATGTT
GGTGTAGATGAACTACATAAGCTTCTTAAAGAAGAAATTACAGCATTGCTTTTAGAAAATCCTCATGCTGATACGGGGAA
TATAGATGAGGCTAAAAAACCTTACGTAATAATGGTGGTGGGAGTCAATGGAGTAGGTAAAACAACTACGATAGGTAAAT
TAGCACATCAGTTTAAAACACAGGGTAAAAAAGTGGTGCTAGGAGCAGCAGATACTTTTAGAGCGGCGGCGGTGGACCAG
CTGGTGATATGGAGTGAGCGAGTAGGTGTACCTATTGTAAAGCAAAATATGGGTTCTGACCCTGCTTCTGTTGCCTTTGA
TACAGTGCAATCTGCAGTAGCTCAAAATGCAGATGTAGTACTTATAGATACAGCTGGGAGGCTTCATAATAAAGTAAATC
TAATGAATGAATTGTCTAAGATAAAAAGAGTAATGCAGAAAGTAATTCCTGATGCTCCACATGAGGTTTTATTGGTATTA
GACGGTTCTACAGGGCAAAATGCATTTGAACAAGCTAAGCAATTTACTGCTGCAACAGAAGTTAATGCTCTTGCGGTTAC
AAAATTAGATGGTACAGCAAAAGGAGGTGTGGTTATTGGTATTTCTGATCAATTTCAAGTTCCTGTAAAATATATAGGGG
TAGGAGAGAAGATGGAAGACTTGCAGTTATTTAATGGCACGGAGTTTGTTGACTCTCTCTTCAAGAAAAAGAGTTGA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(115-314)

SRP54-type protein, helical bundle domain (SRP54_N)

(28-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4TAL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

45.515

94.654

0.431