Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   J6O11_RS02250 Genome accession   NZ_CP081774
Coordinates   478891..479568 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain 0H24     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 473891..484568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J6O11_RS02235 (J6O11_02220) chiA 475258..477951 (+) 2694 WP_000773174.1 bifunctional chitinase/lysozyme -
  J6O11_RS02240 (J6O11_02225) bfd 478120..478314 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  J6O11_RS02245 (J6O11_02230) bfr 478386..478862 (+) 477 WP_000675504.1 bacterioferritin -
  J6O11_RS02250 (J6O11_02235) pilD 478891..479568 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  J6O11_RS02255 (J6O11_02240) gspM 479568..480029 (-) 462 WP_001549764.1 type II secretion system protein GspM -
  J6O11_RS02260 (J6O11_02245) gspL 480026..481189 (-) 1164 WP_001115235.1 type II secretion system protein GspL -
  J6O11_RS02265 (J6O11_02250) gspK 481204..482187 (-) 984 WP_001058517.1 type II secretion system minor pseudopilin GspK -
  J6O11_RS02270 (J6O11_02255) gspJ 482180..482767 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  J6O11_RS02275 (J6O11_02260) gspI 482760..483137 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  J6O11_RS02280 (J6O11_02265) gspH 483134..483643 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  J6O11_RS02285 (J6O11_02270) gspG 483651..484088 (-) 438 WP_001202880.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=523943 J6O11_RS02250 WP_000178161.1 478891..479568(-) (pilD) [Escherichia coli strain 0H24]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=523943 J6O11_RS02250 WP_000178161.1 478891..479568(-) (pilD) [Escherichia coli strain 0H24]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378