Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   J6O52_RS02235 Genome accession   NZ_CP081742
Coordinates   476895..477572 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain CX15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 471895..482572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J6O52_RS02220 (J6O52_02210) chiA 473262..475955 (+) 2694 WP_000773174.1 bifunctional chitinase/lysozyme -
  J6O52_RS02225 (J6O52_02215) bfd 476124..476318 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  J6O52_RS02230 (J6O52_02220) bfr 476390..476866 (+) 477 WP_000675504.1 bacterioferritin -
  J6O52_RS02235 (J6O52_02225) pilD 476895..477572 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  J6O52_RS02240 (J6O52_02230) gspM 477572..478033 (-) 462 WP_001549764.1 type II secretion system protein GspM -
  J6O52_RS02245 (J6O52_02235) gspL 478030..479193 (-) 1164 WP_001115235.1 type II secretion system protein GspL -
  J6O52_RS02250 (J6O52_02240) gspK 479208..480191 (-) 984 WP_001058517.1 type II secretion system minor pseudopilin GspK -
  J6O52_RS02255 (J6O52_02245) gspJ 480184..480771 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  J6O52_RS02260 (J6O52_02250) gspI 480764..481141 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  J6O52_RS02265 (J6O52_02255) gspH 481138..481647 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  J6O52_RS02270 (J6O52_02260) gspG 481655..482092 (-) 438 WP_001202880.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=523689 J6O52_RS02235 WP_000178161.1 476895..477572(-) (pilD) [Escherichia coli strain CX15]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=523689 J6O52_RS02235 WP_000178161.1 476895..477572(-) (pilD) [Escherichia coli strain CX15]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378