Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   U471_RS09810 Genome accession   NC_022653
Coordinates   2078614..2079750 (+) Length   378 a.a.
NCBI ID   WP_012117791.1    Uniprot ID   A7Z5Q9
Organism   Bacillus amyloliquefaciens CC178     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 2073614..2084750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U471_RS09785 (U471_20290) phyC 2073659..2074810 (-) 1152 WP_012117785.1 3-phytase -
  U471_RS09790 (U471_20300) - 2075126..2075551 (+) 426 WP_003153663.1 SDR family NAD(P)-dependent oxidoreductase -
  U471_RS19740 (U471_20310) sprB 2075585..2075761 (-) 177 WP_003153662.1 recombination directionality factor SprB -
  U471_RS09795 (U471_20320) - 2076123..2076380 (-) 258 WP_023356967.1 DUF3221 domain-containing protein -
  U471_RS20130 - 2076922..2077158 (+) 237 WP_077411073.1 hypothetical protein -
  U471_RS09800 - 2077284..2077658 (+) 375 WP_007407921.1 YoqO family protein -
  U471_RS20320 - 2077675..2077753 (-) 79 Protein_1920 hypothetical protein -
  U471_RS09805 - 2077892..2078344 (-) 453 WP_012117790.1 hypothetical protein -
  U471_RS09810 (U471_20340) rapF 2078614..2079750 (+) 1137 WP_012117791.1 Rap family tetratricopeptide repeat protein Regulator
  U471_RS09815 - 2079740..2079922 (+) 183 WP_041482200.1 hypothetical protein -
  U471_RS09820 - 2079953..2080582 (-) 630 Protein_1924 UV damage repair protein UvrX -
  U471_RS09825 (U471_20360) - 2080645..2082057 (-) 1413 WP_012117793.1 MBL fold metallo-hydrolase -
  U471_RS09830 (U471_20370) - 2082216..2083415 (-) 1200 WP_012117794.1 FAD/NAD(P)-binding oxidoreductase -
  U471_RS09835 (U471_20380) - 2083594..2084240 (-) 647 Protein_1927 response regulator -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 44525.17 Da        Isoelectric Point: 5.0394

>NTDB_id=52362 U471_RS09810 WP_012117791.1 2078614..2079750(+) (rapF) [Bacillus amyloliquefaciens CC178]
MEQLISSSKVGLKINEWYKYIRLFSVADAEILKDEVEQEIEHMEEDQDLLLYYSLMCFRHQLMLDYLEPKTMNEERPKVS
DLLEKIESKQSDLKGILEYYFNFFHGMYEFEQYEYLKAISFYNRAEKKLAVVPDEIERAEFHYKVAEVYYHMKQTHMSMH
HIVQAIETYKAHETYSIRVIQCSFVIGLNYLDMEYPEKAIPHFMNALKQAQEIDMPRLIGSSLYNLGLCSFTTGNMEKAL
EYFKEGVSIYQSNSLHNSSRLLDILLMLAKTHFKLFNTVEGKQVCKQGLTLSKALNDDVLGKMFDFLMGLFVENDKDRLF
KILDYLESKAMLSDVEDLATDAAMYYNVNEDHKVATAFYEKVLYARKQIQRGDCLYEI

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=52362 U471_RS09810 WP_012117791.1 2078614..2079750(+) (rapF) [Bacillus amyloliquefaciens CC178]
TTGGAGCAATTGATCTCTTCTTCAAAAGTTGGCTTAAAAATCAATGAATGGTACAAATACATAAGATTGTTCAGTGTTGC
AGATGCAGAAATTCTAAAGGATGAAGTTGAACAAGAAATAGAGCATATGGAAGAAGATCAAGACTTATTGTTGTACTATT
CGCTCATGTGTTTTAGACATCAATTAATGCTTGATTATCTTGAACCAAAAACCATGAATGAAGAACGCCCAAAAGTTTCA
GACTTATTAGAAAAGATCGAAAGTAAACAAAGTGATCTAAAAGGAATTCTTGAATATTATTTTAACTTTTTTCATGGGAT
GTATGAATTTGAACAATACGAATATCTTAAGGCAATAAGTTTTTACAACCGAGCTGAAAAGAAGTTAGCCGTTGTCCCCG
ATGAAATAGAGCGAGCTGAATTTCACTATAAGGTAGCTGAAGTGTACTATCACATGAAGCAAACCCACATGTCTATGCAT
CATATCGTTCAAGCAATAGAAACCTACAAAGCGCACGAAACGTACTCAATAAGAGTAATCCAATGTTCATTCGTCATCGG
TCTGAATTATTTAGACATGGAGTATCCAGAAAAAGCAATACCGCATTTCATGAATGCATTAAAACAAGCACAAGAAATTG
ATATGCCACGGTTAATAGGTTCTTCTTTGTACAATCTCGGTTTATGCTCATTTACAACAGGAAATATGGAGAAAGCATTG
GAGTATTTTAAAGAAGGCGTCTCAATTTATCAAAGTAATTCTCTTCACAATTCTAGCCGACTACTAGACATACTTCTAAT
GTTGGCAAAAACGCACTTTAAATTGTTCAACACGGTTGAGGGAAAACAGGTTTGTAAACAAGGTCTCACGCTATCAAAGG
CGTTGAACGATGACGTATTAGGAAAAATGTTTGATTTTCTTATGGGTTTATTCGTTGAGAATGACAAAGACCGACTTTTT
AAAATCTTAGATTACTTAGAATCAAAAGCTATGCTTTCAGATGTGGAAGACCTAGCGACTGATGCAGCAATGTATTATAA
TGTAAATGAAGACCATAAAGTAGCTACCGCCTTTTATGAGAAAGTGCTTTATGCCCGAAAACAAATTCAGAGAGGGGATT
GTCTTTATGAAATTTAA

Domains

Predicted by InterProScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(196-252)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7Z5Q9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

45.669

100

0.46
  rapC Bacillus subtilis subsp. subtilis str. 168

45.767

100

0.458

Multiple sequence alignment