Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   J6O42_RS01435 Genome accession   NZ_CP081711
Coordinates   305286..306779 (+) Length   497 a.a.
NCBI ID   WP_001040655.1    Uniprot ID   -
Organism   Escherichia coli strain AH21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 300286..311779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J6O42_RS01405 (J6O42_01395) tusA 300533..300778 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  J6O42_RS01410 (J6O42_01400) zntA 300880..303078 (-) 2199 WP_000106552.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  J6O42_RS01415 (J6O42_01405) yhhN 303152..303778 (-) 627 WP_000964718.1 lysoplasmalogenase -
  J6O42_RS01420 (J6O42_01410) yhhM 303919..304278 (+) 360 WP_000042895.1 DUF2500 domain-containing protein -
  J6O42_RS01425 (J6O42_01415) yhhL 304281..304550 (-) 270 WP_001311191.1 DUF1145 family protein -
  J6O42_RS01430 (J6O42_01420) rsmD 304540..305136 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  J6O42_RS01435 (J6O42_01425) pilA 305286..306779 (+) 1494 WP_001040655.1 signal recognition particle-docking protein FtsY Machinery gene
  J6O42_RS01440 (J6O42_01430) ftsE 306782..307450 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  J6O42_RS01445 (J6O42_01435) ftsX 307443..308501 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  J6O42_RS01450 (J6O42_01440) rpoH 308746..309600 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  J6O42_RS01455 (J6O42_01445) livJ 309871..310974 (+) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  J6O42_RS01460 (J6O42_01450) panM 311162..311545 (-) 384 WP_000778768.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 54528.32 Da        Isoelectric Point: 4.1560

>NTDB_id=523348 J6O42_RS01435 WP_001040655.1 305286..306779(+) (pilA) [Escherichia coli strain AH21]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELETALA
AEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIADV
GVETTRKIITNLTEGASRKQLRDAEALYGQLKEEMGEILAKVDEPLNVEGKAPFVILMVGVNGVGKTTTIGKLARQFEQQ
GKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKKI
VRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDL
RPFKADDFIEALFARED

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=523348 J6O42_RS01435 WP_001040655.1 305286..306779(+) (pilA) [Escherichia coli strain AH21]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCCTCTGAACAAGCCGTTGAAG
AGCAGCCGCAAGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATTACCGACGAAGAGCTGGAAACGGCGCTGGCT
GCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCA
GGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCGGAT
TTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTG
GGTGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGACGCCGAGGCGCT
CTATGGCCAGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGT
TTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAGCAG
GGTAAATCGGTGATGCTGGCGGCGGGTGATACTTTCCGTGCAGCTGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCGCAA
CAATATTCCGGTGATTGCCCAGCATACCGGGGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAGCGC
GTAATATCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATC
GTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGC
GGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGAAAG
GCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGATTTG
CGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(293-493)

SRP54-type protein, helical bundle domain (SRP54_N)

(210-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.698

77.264

0.376