Detailed information NTG0052294

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: U471_RS01530
• Genome accession: NC_022653
• Coordinates: 275409..276530 (+)
• Length: 373 a.a.
• NCBI ID: WP_011996281.1
• Uniprot ID: A7Z131
• Organism: Bacillus amyloliquefaciens CC178
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 270409..281530

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U471_RS01510 (U471_02630)	-	270718..271713 (-)	996	WP_007409036.1	DUF5105 domain-containing protein	-
U471_RS01515 (U471_02640)	-	271840..273240 (+)	1401	WP_041482174.1	YcdB/YcdC domain-containing protein	-
U471_RS01520 (U471_02650)	-	273441..274799 (+)	1359	WP_023356694.1	YcdB/YcdC domain-containing protein	-
U471_RS01525 (U471_02660)	-	274796..275299 (-)	504	WP_011996280.1	M15 family metallopeptidase	-
U471_RS01530 (U471_02670)	rapF	275409..276530 (+)	1122	WP_011996281.1	Rap family tetratricopeptide repeat protein	Regulator
U471_RS01535 (U471_02680)	-	276646..277425 (+)	780	WP_011996282.1	glucose 1-dehydrogenase	-
U471_RS01540 (U471_02690)	-	277509..279188 (+)	1680	WP_011996283.1	glycoside hydrolase family 13 protein	-
U471_RS01545 (U471_02700)	-	279374..280333 (+)	960	WP_011996284.1	metal ABC transporter substrate-binding protein	-
U471_RS01550 (U471_02710)	-	280388..281083 (+)	696	WP_011996285.1	metal ABC transporter ATP-binding protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44317.53 Da
• Isoelectric Point: 5.0806

>NTDB_id=52294 U471_RS01530 WP_011996281.1 275409..276530(+) (rapF) [Bacillus amyloliquefaciens CC178]
MKSKIPSEEVAVKMNEWYKLIRAFEADQAEALKREIQYELEDMEENQDLLLYFSLMEFRHRIMLDKLKPMKDGDCQPPFS
EMLTEIEMNQQKLTGLLEYYFYYFRGMYEFKQKNFISAISHYKRAEEKLEYVEDDIEKAEFLYKISEVYYHIKQTFFSMN
YAGQALDIYKKYELYGMRRVQCKFIIAGNLMDAQQHKKALEYLECALEHAERLDDQYMIAAAFYNIGHCMYSLGDFCKAA
EDFERAAAGFEAHSFRQVIQAVFSLTHIYFKTEKYSEAAASCARGMRSAAEWEDEIYMTKFRLIKELYQGEGGQDSLKEY
FDILESKELLADTEELLQDAAHFFHKRENYKAASYFFSRLVGVQKRLQMNNYA

Nucleotide

• Length: 1122 bp

>NTDB_id=52294 U471_RS01530 WP_011996281.1 275409..276530(+) (rapF) [Bacillus amyloliquefaciens CC178]
ATGAAATCCAAGATTCCATCTGAAGAAGTGGCCGTAAAGATGAATGAATGGTATAAGCTGATCCGCGCGTTTGAAGCCGA
TCAAGCTGAAGCGCTGAAGCGGGAGATTCAATATGAACTCGAAGATATGGAAGAGAACCAGGATTTATTGTTGTATTTTT
CATTGATGGAATTTCGGCACCGCATCATGCTGGATAAATTAAAACCCATGAAAGACGGCGACTGCCAGCCTCCGTTCTCT
GAAATGCTCACTGAGATTGAGATGAACCAGCAAAAGCTGACCGGCTTACTGGAATATTATTTTTACTATTTCAGGGGAAT
GTATGAATTTAAGCAGAAAAACTTCATCTCAGCGATTTCTCATTATAAGCGGGCTGAGGAAAAGCTTGAATATGTGGAAG
ATGACATAGAGAAAGCCGAGTTTCTGTATAAAATTTCTGAGGTGTACTATCATATCAAGCAGACTTTCTTTTCGATGAAT
TACGCGGGGCAGGCGCTTGATATTTACAAGAAATATGAATTATACGGAATGAGAAGGGTGCAGTGCAAATTTATCATTGC
CGGAAATCTGATGGACGCCCAGCAGCATAAAAAAGCGCTGGAATACCTCGAATGCGCCTTGGAACACGCGGAGCGGCTTG
ATGATCAATATATGATTGCTGCGGCCTTTTATAATATCGGGCACTGTATGTACAGTCTGGGTGACTTTTGCAAGGCGGCG
GAGGATTTTGAACGGGCGGCTGCCGGATTTGAAGCACACAGCTTCCGGCAGGTCATCCAGGCTGTTTTTTCACTCACGCA
TATCTACTTTAAAACGGAAAAATACAGTGAAGCCGCCGCCAGCTGCGCGCGGGGCATGAGAAGCGCGGCGGAGTGGGAAG
ATGAGATCTATATGACGAAATTCCGTCTGATTAAAGAGCTTTATCAGGGGGAAGGCGGACAGGATTCGCTCAAAGAGTAT
TTTGACATTCTGGAATCAAAAGAGCTGTTGGCTGACACAGAGGAACTGCTTCAGGACGCGGCTCATTTCTTTCATAAACG
GGAGAACTACAAAGCGGCATCTTATTTTTTCAGCAGGCTTGTCGGCGTGCAAAAAAGGCTGCAAATGAACAACTACGCAT
AA

Domains

Predicted by InterProScan.

• Tetratricopeptide repeat (TPR_12) (187-251)

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

3D structure

• AlphaFold DB: A7Z131

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.588	99.464	0.424
rapC	Bacillus subtilis subsp. subtilis str. 168	40.054	99.732	0.399