Detailed information NTG0522783

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: K5T46_RS20510
• Genome accession: NZ_CP081489
• Coordinates: 4254480..4255463 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli BL21(DE3)
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 4249480..4260463

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K5T46_RS20495 (K5T46_20495)	dppA	4250623..4252230 (+)	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-
K5T46_RS20500 (K5T46_20500)	dppB	4252538..4253557 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
K5T46_RS20505 (K5T46_20505)	dppC	4253567..4254469 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
K5T46_RS20510 (K5T46_20510)	amiE	4254480..4255463 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
K5T46_RS20515 (K5T46_20515)	dppF	4255460..4256464 (+)	1005	WP_000107012.1	dipeptide ABC transporter ATP-binding subunit DppF	-
K5T46_RS20520 (K5T46_20520)	yhjV	4256494..4257765 (-)	1272	WP_001295225.1	aromatic amino acid transport family protein	-
K5T46_RS20525 (K5T46_20525)	-	4258048..4258314 (-)	267	WP_221840787.1	Bor family protein	-
K5T46_RS20530 (K5T46_20530)	-	4258675..4258869 (-)	195	WP_001415975.1	YlcI/YnfO family protein	-
K5T46_RS20535 (K5T46_20535)	-	4259258..4259833 (+)	576	WP_000453609.1	terminase small subunit	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=522783 K5T46_RS20510 WP_001196486.1 4254480..4255463(+) (amiE) [Escherichia coli BL21(DE3)]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=522783 K5T46_RS20510 WP_001196486.1 4254480..4255463(+) (amiE) [Escherichia coli BL21(DE3)]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCGACCACCGCGCTGGACGTGAC
CATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTGGTGGAAACCGGTGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404