Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   K5T46_RS18195 Genome accession   NZ_CP081489
Coordinates   3811419..3812096 (-) Length   225 a.a.
NCBI ID   WP_000178163.1    Uniprot ID   -
Organism   Escherichia coli BL21(DE3)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3806419..3817096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5T46_RS21990 - 3807926..3808063 (-) 138 Protein_3594 hypothetical protein -
  K5T46_RS18175 (K5T46_18175) - 3808056..3808127 (+) 72 Protein_3595 hypothetical protein -
  K5T46_RS18180 (K5T46_18180) - 3808419..3810479 (+) 2061 WP_000773160.1 carbohydrate-binding protein -
  K5T46_RS18185 (K5T46_18185) bfd 3810648..3810842 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  K5T46_RS18190 (K5T46_18190) bfr 3810914..3811390 (+) 477 WP_000675504.1 bacterioferritin -
  K5T46_RS18195 (K5T46_18195) pilD 3811419..3812096 (-) 678 WP_000178163.1 prepilin peptidase GspO Machinery gene
  K5T46_RS18200 (K5T46_18200) gspM 3812096..3812557 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  K5T46_RS18205 (K5T46_18205) gspL 3812554..3813717 (-) 1164 WP_001115228.1 type II secretion system protein GspL -
  K5T46_RS18210 (K5T46_18210) gspK 3813732..3814715 (-) 984 WP_001058521.1 type II secretion system minor pseudopilin GspK -
  K5T46_RS18215 (K5T46_18215) gspJ 3814708..3815295 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  K5T46_RS18220 (K5T46_18220) gspI 3815288..3815665 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  K5T46_RS18225 (K5T46_18225) gspH 3815662..3816171 (-) 510 WP_001076029.1 type II secretion system minor pseudopilin GspH -
  K5T46_RS18230 (K5T46_18230) gspG 3816179..3816616 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24928.81 Da        Isoelectric Point: 7.9563

>NTDB_id=522774 K5T46_RS18195 WP_000178163.1 3811419..3812096(-) (pilD) [Escherichia coli BL21(DE3)]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTSAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=522774 K5T46_RS18195 WP_000178163.1 3811419..3812096(-) (pilD) [Escherichia coli BL21(DE3)]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTAGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378