Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   JH254_RS01260 Genome accession   NZ_CP066813
Coordinates   246614..248053 (-) Length   479 a.a.
NCBI ID   WP_039040474.1    Uniprot ID   -
Organism   Aeromonas caviae strain Aero52     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 241614..253053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH254_RS01230 (JH254_01230) dagF 241891..242631 (+) 741 WP_043154589.1 2-dehydro-3-deoxy-phosphogluconate aldolase -
  JH254_RS01235 (JH254_01235) - 242737..244641 (+) 1905 WP_143238150.1 BglG family transcription antiterminator -
  JH254_RS01240 (JH254_01240) npr 244657..244929 (-) 273 WP_010675868.1 PTS phosphocarrier protein NPr -
  JH254_RS01245 (JH254_01245) rapZ 244950..245816 (-) 867 WP_010675869.1 RNase adapter RapZ -
  JH254_RS01250 (JH254_01250) ptsN 245857..246303 (-) 447 WP_010675870.1 PTS IIA-like nitrogen regulatory protein PtsN -
  JH254_RS01255 (JH254_01255) hpf 246306..246593 (-) 288 WP_005305957.1 ribosome hibernation promoting factor -
  JH254_RS01260 (JH254_01260) rpoN/rpoN1 246614..248053 (-) 1440 WP_039040474.1 RNA polymerase factor sigma-54 Machinery gene
  JH254_RS01265 (JH254_01265) lptB 248119..248844 (-) 726 WP_010675872.1 LPS export ABC transporter ATP-binding protein -
  JH254_RS01270 (JH254_01270) lptA 248857..249375 (-) 519 WP_010675873.1 lipopolysaccharide transport periplasmic protein LptA -
  JH254_RS01275 (JH254_01275) lptC 249362..249922 (-) 561 WP_039040473.1 LPS export ABC transporter periplasmic protein LptC -
  JH254_RS01280 (JH254_01280) kdsC 249919..250473 (-) 555 WP_200164290.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  JH254_RS01285 (JH254_01285) - 250473..251468 (-) 996 WP_029314845.1 KpsF/GutQ family sugar-phosphate isomerase -
  JH254_RS01290 (JH254_01290) mlaF 251675..252475 (+) 801 WP_069785529.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53761.57 Da        Isoelectric Point: 4.3728

>NTDB_id=522746 JH254_RS01260 WP_039040474.1 246614..248053(-) (rpoN/rpoN1) [Aeromonas caviae strain Aero52]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEAQETSQTETQDASQLDSSDAMAQESMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTATLED
IQAAVSSEEVEVELDEIEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLDEAREVIREHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGIIQSESQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNEAYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=522746 JH254_RS01260 WP_039040474.1 246614..248053(-) (rpoN/rpoN1) [Aeromonas caviae strain Aero52]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCACAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGACAACAACCCCTTGCTGGAGCAAGAGGAGAACGAGG
CGCAGGAAACCAGTCAGACAGAGACACAGGACGCCAGTCAACTCGACTCCAGCGATGCCATGGCCCAGGAGAGCATGCCG
GATGAACTGCCGGTGGACACCACCTGGGACGAGGTCTACTCGGCCGGTACGGCCCAGAGCGCAGGTCCCATCCATGACGG
GGAAGACAGCGTCTATCAGGGGGAGACCACCGAGAACCTGCAGGACTACCTGCTGTGGCAGATGCGGCTCACCCCGTTCA
GTGATGTGGATGCTGCCATCGCCCTTGCCATCATCGATGCCATCGACGAATCCGGGTATCTGACTGCCACCCTCGAGGAT
ATTCAGGCTGCAGTCAGCAGCGAGGAGGTAGAAGTCGAACTGGATGAGATCGAAGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCCCGTTCGGTGCAAGAGTGTCTGCTGATCCAGCTCGGCCAGTATTCGCCAGACCTGCCCT
GGCTCGATGAAGCCAGAGAGGTGATCCGCGAACACATGGACCTGCTCGGCAATCGCGACTACCGCACCCTGGCTCGCAAG
ACCCGCCTCAAGGAGAGCGATCTCAAGGAGGTGCTGGATCTCATCCAGCAACTGGAGCCTAGACCCGGCAACGGCATCAT
CCAGAGTGAATCCCAGTACGTGATCCCGGATGTGTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTTGAACTCAATCCGG
ACTCTGCTCCGCGCATCCGTGTCAACGAGGCCTATGCGGCCATGGCGCGCAATGCCCGCAGCAGCACGGATACCCAGTTC
ATCCGTTCTCATTTGCAGGAGGCCAAGTGGTTCATCAAGAGCCTGGAGAGTCGTAACGACACCCTGCTCAAGGTGGCCAG
CTGCATCGTCGAGCAGCAGCAGGGCTTTTTCGAGTACGGCGAAGAGGCCATGAAACCCATGGTACTCAACGACATCGCGG
AAGCGGTGGAGATGCATGAGTCGACCATTTCACGGGTCACGACCCAGAAGTACATGCATACGCCCCGCGGCATTTTTGAA
CTGAAGTTTTTTTTCTCCAGTCACGTCAATACGGAGGGAGGAGGAGAGTGCTCATCGACCGCTATTCGCGCCCTGATCAA
GAAGCTGGTGACGGCGGAAAACCCCGCCAAGCCGCTTAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.472

100

0.482