Detailed information NTG0522618

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: GIY62_RS08640
• Genome accession: NZ_CP066798
• Coordinates: 1870907..1872064 (+)
• Length: 385 a.a.
• NCBI ID: WP_042626054.1
• Uniprot ID: A0A0B6S059
• Organism: Burkholderia plantarii strain KACC 18964
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1865907..1877064

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GIY62_RS08610 (GIY62_08585)	hisC	1866145..1867212 (+)	1068	WP_304992163.1	histidinol-phosphate transaminase	-
GIY62_RS08615 (GIY62_08590)	-	1867360..1867629 (-)	270	WP_042626050.1	YfhL family 4Fe-4S dicluster ferredoxin	-
GIY62_RS08620 (GIY62_08595)	-	1867852..1868001 (+)	150	WP_158336071.1	hypothetical protein	-
GIY62_RS08625 (GIY62_08600)	-	1868016..1869338 (+)	1323	WP_042626051.1	TIGR00366 family protein	-
GIY62_RS08630 (GIY62_08605)	coaD	1869493..1869993 (-)	501	WP_042626052.1	pantetheine-phosphate adenylyltransferase	-
GIY62_RS08635 (GIY62_08610)	rsmD	1870104..1870718 (-)	615	WP_304992164.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
GIY62_RS08640 (GIY62_08615)	pilA	1870907..1872064 (+)	1158	WP_042626054.1	signal recognition particle-docking protein FtsY	Machinery gene
GIY62_RS08645 (GIY62_08620)	maiA	1872170..1872814 (-)	645	WP_304992165.1	maleylacetoacetate isomerase	-
GIY62_RS08650 (GIY62_08625)	ybiB	1873006..1873983 (+)	978	WP_304992166.1	DNA-binding protein YbiB	-
GIY62_RS08655 (GIY62_08630)	leuA	1874418..1876067 (+)	1650	WP_304992167.1	2-isopropylmalate synthase	-
GIY62_RS08660 (GIY62_08635)	-	1876249..1876635 (-)	387	WP_042626058.1	nuclear transport factor 2 family protein	-

Sequence

Protein

• Length: 385 a.a.
• Molecular weight: 40323.00 Da
• Isoelectric Point: 4.6904

>NTDB_id=522618 GIY62_RS08640 WP_042626054.1 1870907..1872064(+) (pilA) [Burkholderia plantarii strain KACC 18964]
MFSFFKRLIGSKADDASQDAPAADEQGSAAHAPDPAEDAAAPAPAHAAEPATGAAVEPASEPAPAAAEASPASAVEIVPP
PAPEPAAKKSWIARLRSGLSKTSTSLTSVFVNTKIDDELYEELEAALLMSDAGIDATEYLLGTLREKVRAERLSDPLQVK
AALRALLVDLLEPLEKSLMLGRAQPLVMMIAGVNGAGKTTSIGKLAKHLQQFDQSVLLAAGDTFRAAAREQLAIWGERNN
VTVVQQESGDPAAVIFDAVGAARARKIDVVMADTAGRLPTQLHLMEELRKVKRVIGKAQSDAPHEVLLVIDANTGQNALA
QVKAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFSAEEFADALLG

Nucleotide

• Length: 1158 bp

>NTDB_id=522618 GIY62_RS08640 WP_042626054.1 1870907..1872064(+) (pilA) [Burkholderia plantarii strain KACC 18964]
ATGTTCAGCTTCTTCAAACGATTGATCGGCTCGAAGGCCGACGACGCGTCGCAGGACGCGCCGGCGGCCGACGAGCAAGG
CAGCGCGGCTCACGCGCCGGACCCGGCCGAAGATGCCGCCGCGCCCGCGCCGGCACACGCCGCCGAGCCGGCCACCGGGG
CCGCTGTCGAGCCCGCTTCGGAACCGGCCCCCGCCGCCGCCGAAGCCTCACCCGCCAGCGCCGTCGAGATCGTGCCGCCG
CCCGCGCCCGAACCGGCCGCCAAGAAATCCTGGATCGCTCGGCTGCGCTCGGGTCTCTCGAAGACGAGCACGAGCCTCAC
CAGCGTGTTCGTCAACACCAAGATCGACGACGAACTCTACGAGGAACTCGAGGCCGCGCTGCTGATGTCCGACGCCGGCA
TCGACGCGACCGAATACCTGCTCGGCACGCTGCGCGAGAAGGTGCGCGCCGAACGCCTGAGCGATCCGCTGCAGGTGAAG
GCCGCGCTGCGCGCGCTGCTCGTCGACCTGCTCGAGCCGCTCGAGAAGTCGCTGATGCTGGGCCGCGCCCAGCCGCTCGT
GATGATGATCGCGGGCGTCAACGGCGCCGGCAAGACCACCAGCATCGGCAAGCTCGCCAAGCACCTGCAGCAATTCGACC
AGTCGGTGCTGCTGGCGGCCGGCGACACGTTCCGTGCCGCCGCGCGCGAGCAGCTCGCGATCTGGGGCGAGCGCAACAAC
GTGACGGTGGTGCAGCAGGAGAGCGGCGATCCGGCCGCCGTGATCTTCGACGCGGTCGGCGCCGCGCGCGCGCGCAAGAT
CGACGTGGTGATGGCCGACACCGCAGGCCGGCTGCCCACGCAGTTGCACCTGATGGAAGAACTGCGCAAGGTCAAGCGCG
TGATCGGCAAGGCGCAGAGCGACGCGCCGCACGAGGTGCTGCTCGTGATCGACGCGAACACCGGGCAGAACGCGCTCGCG
CAGGTCAAGGCGTTCGACGACGCGCTGGGCCTCACCGGGCTCATCGTCACCAAGCTCGACGGCACCGCGAAGGGCGGCAT
TCTCGCCGCGATCGCGCGCCAGCGGCCGGTGCCCGTCTACTTCATCGGGGTCGGCGAGAAGGTCGAGGACCTGCAGCCGT
TCAGCGCCGAGGAATTCGCCGACGCGCTGCTCGGCTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (186-385)

• SRP54-type protein, helical bundle domain (SRP54_N) (104-164)

3D structure

• AlphaFold DB: A0A0B6S059

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	51.069	100	0.558