Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   JDW18_RS21200 Genome accession   NZ_CP066783
Coordinates   4565280..4566875 (-) Length   531 a.a.
NCBI ID   WP_218241568.1    Uniprot ID   -
Organism   Comamonas fluminis strain CJ34     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4560280..4571875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JDW18_RS21185 - 4560774..4561700 (+) 927 WP_218241565.1 aspartyl/asparaginyl beta-hydroxylase domain-containing protein -
  JDW18_RS21190 - 4561861..4562529 (+) 669 WP_218241566.1 TetR/AcrR family transcriptional regulator -
  JDW18_RS21195 - 4562622..4565000 (-) 2379 WP_218241567.1 membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family -
  JDW18_RS21200 rpoN/rpoN1 4565280..4566875 (-) 1596 WP_218241568.1 RNA polymerase factor sigma-54 Machinery gene
  JDW18_RS21205 lptB 4566943..4567734 (-) 792 WP_218241569.1 LPS export ABC transporter ATP-binding protein -
  JDW18_RS21210 lptA 4567731..4568387 (-) 657 WP_218241570.1 lipopolysaccharide transport periplasmic protein LptA -
  JDW18_RS21215 - 4568499..4569149 (-) 651 WP_218241571.1 thiol:disulfide interchange protein DsbA/DsbL -
  JDW18_RS21220 - 4569310..4570050 (-) 741 WP_218241572.1 SPOR domain-containing protein -
  JDW18_RS21225 argS 4570075..4571784 (-) 1710 WP_218241573.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 531 a.a.        Molecular weight: 59104.60 Da        Isoelectric Point: 4.5816

>NTDB_id=522520 JDW18_RS21200 WP_218241568.1 4565280..4566875(-) (rpoN/rpoN1) [Comamonas fluminis strain CJ34]
MKPGLSLRVSQHLSLTPQLQQSIQLLQLSTLELAQEVEQMLIDNPFLERDAEDAGAPDEIAASLNNSSISEIYQSEKETE
KAQAASESIAEPATAETPENLNWEADGIDGQDGEWGGESSSEWDSPSLSGSGSGSNRDSDDEVDAIDLAREHVSLTDFLH
RQALSLHLGAEDMAALRFLIESLSDDGYLEDPLQELAQSLAGDDFEEREELEQRFSIALKLLQSLEPVGVGARDLAECLQ
LQLRDLIREAEDEGTTQAHMNLLLLAQNICAQPMDLLARRDLRKLTSLCGAREEALREAMALIARLEPRPGRRFMDVERQ
IVVPDVIVRPVRASSGRVDFAVQLNPDVMPKLRVHDIYASVLRRHKNSESHAALQQRLQEARWFIKNIQQRFDTILRVSE
AIVERQKNFFVHGELAMRPLVLRDIADQLGLHESTISRVTTAKYMATPQGTFELKYFFGSGLGTETGGSASSTAVRALIK
QFVDAEDTKKPLSDAKLADMLKEQGIECARRTVAKYREQLKIPTTTLRKAL

Nucleotide


Download         Length: 1596 bp        

>NTDB_id=522520 JDW18_RS21200 WP_218241568.1 4565280..4566875(-) (rpoN/rpoN1) [Comamonas fluminis strain CJ34]
ATGAAACCAGGGCTGTCGCTACGCGTATCGCAACATCTGTCGCTGACGCCGCAGCTGCAGCAATCGATTCAGCTGCTGCA
GCTGTCCACTCTGGAGCTGGCGCAGGAAGTCGAGCAGATGCTGATCGACAACCCTTTTCTTGAGCGCGATGCGGAGGATG
CGGGCGCTCCTGATGAAATAGCAGCCAGCCTAAACAACTCAAGCATTTCAGAGATATATCAATCAGAAAAAGAGACTGAG
AAAGCGCAGGCAGCTTCAGAATCCATAGCAGAGCCCGCCACCGCAGAAACCCCGGAAAACCTGAACTGGGAAGCCGATGG
CATTGATGGCCAGGATGGCGAGTGGGGCGGGGAATCCAGCAGCGAGTGGGATAGCCCATCGCTCAGTGGCAGCGGCTCTG
GCAGCAACCGGGACAGTGACGATGAGGTCGATGCCATTGACCTGGCCCGCGAGCATGTGAGCCTGACCGACTTTCTGCAT
CGCCAGGCGCTGAGCCTGCACCTCGGGGCAGAAGACATGGCTGCGCTGCGTTTTCTGATCGAGTCGCTCAGTGACGACGG
CTACCTTGAAGACCCCTTGCAAGAGCTGGCGCAAAGCCTGGCTGGTGATGACTTTGAGGAGCGTGAAGAGCTGGAGCAGC
GCTTTTCCATTGCACTCAAACTGTTGCAAAGTCTGGAGCCCGTAGGCGTTGGCGCGCGCGACCTGGCTGAATGCCTGCAG
CTGCAACTGCGCGATCTGATCCGCGAGGCCGAGGACGAGGGCACGACACAAGCGCACATGAATCTGCTGCTTCTGGCGCA
AAACATCTGCGCCCAGCCCATGGACCTGCTGGCGCGGCGTGATCTGCGCAAGCTCACCAGCCTGTGCGGCGCCCGTGAAG
AGGCTTTGCGCGAAGCCATGGCACTGATTGCACGGCTGGAGCCACGGCCAGGGCGCCGGTTTATGGATGTGGAGCGCCAG
ATCGTGGTGCCTGATGTCATCGTGCGCCCTGTGCGCGCCAGTTCAGGCCGCGTTGATTTTGCCGTGCAGCTCAACCCCGA
TGTGATGCCCAAGCTGCGTGTGCACGATATCTACGCCAGCGTGCTGCGCCGCCACAAAAACAGCGAAAGCCATGCCGCAC
TGCAGCAGCGTCTGCAGGAGGCCCGCTGGTTCATCAAGAACATTCAGCAGCGTTTCGACACCATCTTGCGCGTCTCTGAA
GCGATTGTGGAGCGGCAGAAAAACTTCTTCGTGCATGGCGAGTTGGCCATGCGCCCGCTGGTGTTGCGCGATATTGCAGA
CCAGTTGGGCCTGCATGAATCCACCATCAGCCGTGTGACAACCGCCAAGTACATGGCCACGCCGCAAGGCACATTCGAGC
TGAAGTACTTCTTTGGCTCAGGCCTGGGCACAGAAACCGGCGGCAGCGCGTCCAGCACGGCAGTGCGTGCGCTGATCAAG
CAGTTTGTGGACGCGGAAGACACGAAAAAGCCGTTATCAGACGCCAAACTGGCCGATATGCTCAAGGAGCAAGGCATCGA
ATGTGCCCGCCGCACCGTCGCCAAATACCGCGAACAGCTCAAGATACCCACGACCACGCTGCGCAAGGCGCTGTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(373-529)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(148-358)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

50.847

100

0.508