Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RP6297_RS01380 Genome accession   NZ_CP066771
Coordinates   290749..292260 (-) Length   503 a.a.
NCBI ID   WP_012761098.1    Uniprot ID   A0A1C0XGV8
Organism   Ralstonia pickettii strain K-288     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 285749..297260
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RP6297_RS01350 (RP6297_00272) - 286240..286893 (-) 654 WP_012761094.1 LON peptidase substrate-binding domain-containing protein -
  RP6297_RS01355 (RP6297_00273) rapZ 286993..287886 (-) 894 WP_012761095.1 RNase adapter RapZ -
  RP6297_RS01360 (RP6297_00274) - 287981..288310 (-) 330 WP_037027670.1 PsiF family protein -
  RP6297_RS01365 (RP6297_00275) hprK 288495..289469 (-) 975 WP_004633393.1 HPr(Ser) kinase/phosphatase -
  RP6297_RS01370 (RP6297_00276) ptsN 289543..289998 (-) 456 WP_012761097.1 PTS IIA-like nitrogen regulatory protein PtsN -
  RP6297_RS01375 (RP6297_00277) hpf 290345..290698 (-) 354 WP_004633388.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  RP6297_RS01380 (RP6297_00278) rpoN/rpoN1 290749..292260 (-) 1512 WP_012761098.1 RNA polymerase factor sigma-54 Machinery gene
  RP6297_RS01385 (RP6297_00279) lptB 292369..293157 (-) 789 WP_037027669.1 LPS export ABC transporter ATP-binding protein -
  RP6297_RS01390 (RP6297_00280) lptA 293188..293775 (-) 588 WP_037027668.1 lipopolysaccharide transport periplasmic protein LptA -
  RP6297_RS01395 (RP6297_00281) lptC 293835..294467 (-) 633 WP_012761100.1 LPS export ABC transporter periplasmic protein LptC -
  RP6297_RS01400 (RP6297_00282) - 294472..295056 (-) 585 WP_037027667.1 HAD family hydrolase -
  RP6297_RS01405 (RP6297_00283) - 295073..296056 (-) 984 WP_037027665.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 503 a.a.        Molecular weight: 55712.03 Da        Isoelectric Point: 4.9013

>NTDB_id=522447 RP6297_RS01380 WP_012761098.1 290749..292260(-) (rpoN/rpoN1) [Ralstonia pickettii strain K-288]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVNALQSNSATPLPTE
SPPPAPNGGDSSNSSDSSYENGHDNGADFDSDYGSDRSDWSLDDFARKPQSDEDDRAPLQLREAEQSLREFLMEQLAPLK
LSMRDKGLVIFLIESLDDEGYLSGTLDEILADLPTELEVELDELQAALRMLQSFDPPGIGARSAAECLSLQLHRLDSPAK
ALALVIVNQHLELLAARDYTRLKKALSVDEPALKAAHELIRSLTPFPGHAFGRAEADFVVPDVIVRKTSAGWMAQLNPDV
MPRLRINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADT
LGLHESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVV
ARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1512 bp        

>NTDB_id=522447 RP6297_RS01380 WP_012761098.1 290749..292260(-) (rpoN/rpoN1) [Ralstonia pickettii strain K-288]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCTCAGCATCTCGCGCTCACGCCGCAGTTGCAGCAATCGATCCGACTGCTGCA
GTTGTCGACCATCGAACTGCAGCAGGAAGTTGAACAGGCCCTGACCGAAAACCCGCTGCTGGAGCGGGAAAACGAGTGGC
TCGACACCTCCGCCCGCATTGGCGCAGACGGCTCCGTCAACGCGCTGCAAAGCAACAGCGCCACGCCCCTTCCCACTGAA
TCACCTCCGCCCGCACCCAACGGCGGCGACAGCAGCAACAGCTCGGACAGCAGCTACGAAAACGGCCACGACAACGGTGC
CGATTTCGACAGCGATTACGGCTCCGACCGCTCCGACTGGAGCCTTGACGACTTCGCCCGCAAACCGCAATCCGATGAAG
ACGATCGTGCTCCGCTGCAGTTGCGCGAAGCCGAACAAAGCCTGCGCGAATTCCTCATGGAACAGCTCGCGCCGCTCAAG
CTCTCGATGCGCGACAAGGGCCTCGTCATCTTCCTGATCGAGTCGCTCGACGACGAAGGCTATCTGTCGGGCACGCTCGA
CGAAATCCTCGCAGACCTACCGACCGAACTCGAAGTCGAACTGGATGAACTGCAAGCCGCGCTGCGCATGCTGCAGAGCT
TTGATCCACCGGGCATTGGCGCGCGCTCTGCAGCCGAATGCCTCTCATTGCAACTGCATCGGCTCGACTCGCCCGCGAAG
GCGCTGGCGCTCGTCATCGTCAATCAGCATCTCGAACTGCTGGCCGCGCGCGACTACACGCGCCTGAAGAAGGCCCTGTC
CGTCGATGAGCCCGCGTTGAAGGCAGCGCATGAGCTGATTCGCTCACTGACACCATTCCCGGGTCATGCTTTCGGCCGTG
CTGAAGCAGATTTCGTGGTGCCCGACGTGATCGTGCGAAAAACGAGCGCAGGCTGGATGGCGCAACTGAACCCCGACGTC
ATGCCGCGCTTGCGCATCAACGACATGTACGCGCAGATTCTGCGCAGCAGCCGTGGCGAGTCGGGCGCCGCCAATCTGCA
GCAGAAGCTGCAGGAAGCACGTTGGCTGATCAAAAACATTCAACAGCGCTTCGATACGATCCTGCGTGTCTCGCAAGCGA
TTGTCGAGCGTCAAAAGAGCTTCTTCACGCACGGCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACA
CTGGGTCTACACGAGTCCACGATCTCCCGTGTGACAACCAACAAATACATGGCGACCCCGATGGGGACGTTCGAGTTGAA
GTACTTCTTCGGCAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAAC
TTGTAGGAGCCGAAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAACTGTTAGGTGAACAAGGATTCGTGGTG
GCCCGTCGGACGGTGGCCAAATACCGCGAAGCCCTCAAGATCCCTGCAGTGAATTTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(141-330)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-501)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C0XGV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

92.843

100

0.928