Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   JFN94_RS14325 Genome accession   NZ_CP066769
Coordinates   3059653..3061158 (+) Length   501 a.a.
NCBI ID   WP_124827897.1    Uniprot ID   A0A7T6VE31
Organism   Burkholderia anthina strain BJQ0011     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3054653..3066158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JFN94_RS14300 (JFN94_14300) kdsD 3055886..3056869 (+) 984 WP_175858886.1 arabinose 5-phosphate isomerase KdsD -
  JFN94_RS14305 (JFN94_14305) - 3056869..3057405 (+) 537 WP_175750993.1 HAD family hydrolase -
  JFN94_RS14310 (JFN94_14310) lptC 3057409..3058023 (+) 615 WP_096507356.1 LPS export ABC transporter periplasmic protein LptC -
  JFN94_RS14315 (JFN94_14315) lptA 3058044..3058709 (+) 666 WP_124827899.1 lipopolysaccharide transport periplasmic protein LptA -
  JFN94_RS14320 (JFN94_14320) lptB 3058706..3059482 (+) 777 WP_124827898.1 LPS export ABC transporter ATP-binding protein -
  JFN94_RS14325 (JFN94_14325) rpoN/rpoN1 3059653..3061158 (+) 1506 WP_124827897.1 RNA polymerase factor sigma-54 Machinery gene
  JFN94_RS14330 (JFN94_14330) hpf 3061318..3061677 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  JFN94_RS14335 (JFN94_14335) - 3061873..3062385 (+) 513 WP_059451610.1 PTS sugar transporter subunit IIA -
  JFN94_RS14340 (JFN94_14340) hprK 3062515..3063483 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  JFN94_RS14345 (JFN94_14345) rapZ 3063559..3064467 (+) 909 WP_124827896.1 RNase adapter RapZ -
  JFN94_RS14350 (JFN94_14350) - 3064494..3065129 (+) 636 WP_124827895.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54712.85 Da        Isoelectric Point: 4.8767

>NTDB_id=522443 JFN94_RS14325 WP_124827897.1 3059653..3061158(+) (rpoN/rpoN1) [Burkholderia anthina strain BJQ0011]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQSAEPAA
ANGSGQSGERAERDEPAGADEYDGYASGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTASLDEVLADLPAELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKSGQNWLAELNPEVMP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=522443 JFN94_RS14325 WP_124827897.1 3059653..3061158(+) (rpoN/rpoN1) [Burkholderia anthina strain BJQ0011]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATTTGGCGCTGACGCCGCAGCTACAGCAGTCGATCCGGCTTCTGCA
GTTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAATCCGCTGCTCGAAAATGACGATGAATGGA
TCGCGAGTCCGCTGCGTGTCGCGGCGGATGGATCGCTGATCGCGCAGACGCCGCCCGCGCAGAGCGCCGAACCGGCCGCC
GCGAACGGCAGCGGCCAGTCGGGCGAGCGCGCCGAGCGCGACGAACCGGCCGGCGCCGACGAATACGACGGCTACGCATC
CGGCGACGGCAACGACGGCCCGCAGTGGAATCTCGACGAGTTCGGCCGCTCGAGCGGCGCATCGGACGACGACGACCTGC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCATCTCTCCGCGCAACTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCGTCGCTCGACGAAGTGCT
CGCCGATCTGCCGGCCGAACTTGAAATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCCG
CCGGGGTCGGTGCGCGCTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCCACCCGCACGCTC
GCGCTCGAAATCGTGTCGCAGCATCTCGAGCTGCTCGCCGCGCGCGATTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGTTCGCTGGAGCCGTTCCCGGGCGCCGCATACGGCAAGGCCGAAG
CGGACTACGTCGTACCCGACATCATCGTGAAGAAGTCCGGCCAGAACTGGCTCGCGGAACTCAATCCGGAAGTGATGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGTTGAAACAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGCTTCGACACGATCCTGCGCGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACAGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCACACGTTTCGACCGACACCGGAGGCGCCGCTTCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTTGCGCGC
CGCACGGTCGCCAAGTATCGCGAAGCCCTCAAAATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T6VE31

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.775

100

0.695