Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   JG536_RS14165 Genome accession   NZ_CP066762
Coordinates   3071006..3072511 (+) Length   501 a.a.
NCBI ID   WP_175670613.1    Uniprot ID   -
Organism   Burkholderia ambifaria strain BJQ0010     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3066006..3077511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JG536_RS14140 (JG536_14140) kdsD 3067226..3068209 (+) 984 WP_126228526.1 arabinose 5-phosphate isomerase KdsD -
  JG536_RS14145 (JG536_14145) - 3068209..3068745 (+) 537 WP_105833002.1 HAD family hydrolase -
  JG536_RS14150 (JG536_14150) lptC 3068749..3069363 (+) 615 WP_126228527.1 LPS export ABC transporter periplasmic protein LptC -
  JG536_RS14155 (JG536_14155) lptA 3069384..3070049 (+) 666 WP_011657963.1 lipopolysaccharide transport periplasmic protein LptA -
  JG536_RS14160 (JG536_14160) lptB 3070046..3070822 (+) 777 WP_175656273.1 LPS export ABC transporter ATP-binding protein -
  JG536_RS14165 (JG536_14165) rpoN/rpoN1 3071006..3072511 (+) 1506 WP_175670613.1 RNA polymerase factor sigma-54 Machinery gene
  JG536_RS14170 (JG536_14170) hpf 3072669..3073028 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  JG536_RS14175 (JG536_14175) - 3073225..3073737 (+) 513 WP_175670654.1 PTS sugar transporter subunit IIA -
  JG536_RS14180 (JG536_14180) hprK 3073867..3074835 (+) 969 WP_011657967.1 HPr(Ser) kinase/phosphatase -
  JG536_RS14185 (JG536_14185) rapZ 3074911..3075819 (+) 909 WP_041489859.1 RNase adapter RapZ -
  JG536_RS14190 (JG536_14190) - 3075846..3076481 (+) 636 WP_175665596.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54758.87 Da        Isoelectric Point: 4.8795

>NTDB_id=522429 JG536_RS14165 WP_175670613.1 3071006..3072511(+) (rpoN/rpoN1) [Burkholderia ambifaria strain BJQ0010]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENEDEWITSPLRVASDGSLIAQGPSGQNIEPGP
ANGSSPSSERAERDEPAGADEYDGYGSGDAGDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTASLDEVLSDLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVMP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=522429 JG536_RS14165 WP_175670613.1 3071006..3072511(+) (rpoN/rpoN1) [Burkholderia ambifaria strain BJQ0010]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACCTGGCGCTGACCCCGCAACTGCAGCAGTCGATCCGGCTCCTGCA
ACTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAAAATCCGTTGCTCGAAAACGAAGACGAATGGA
TCACGAGTCCGCTGCGTGTCGCGTCGGACGGATCGCTGATCGCGCAGGGGCCTTCCGGACAGAACATCGAGCCGGGCCCC
GCGAACGGCAGCAGCCCGTCGAGCGAGCGCGCCGAGCGCGACGAGCCGGCCGGCGCCGACGAATACGACGGCTACGGGTC
GGGCGACGCCGGCGACGGCCCGCAGTGGAATCTGGACGAGTACGGCCGCTCGAGCGGCGCATCGGACGATGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCATCTGTCCGCGCAATTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCGTCGCTCGACGAAGTGCT
CTCCGACCTGCCGCCGGAACTGGAAGTCGACTGCGACGAACTGAATGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGGGTCGGGGCCCGCTCGGCCTCCGAGTGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCCACCCGCACGCTC
GCGCTGGAGATCGTGTCGCAGCACCTCGAGCTGCTCGCCGCGCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAACTCAATCCGGAAGTGATGCCG
CGGCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGGTCGCTCAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCCATTGTCG
AACGTCAAAAGAATTTCTTCGCACATGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACAGGCAAGTACATGCTGACCCCGTTCGGTACGCTGGAATTCAAGTACTT
CTTCGGGTCACACGTTTCGACCGACACCGGAGGCGCCGCATCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTTGCGCGC
CGCACGGTTGCCAAGTATCGCGAAGCCCTCAAAATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.515

100

0.691