Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   JG535_RS05535 Genome accession   NZ_CP066746
Coordinates   1085179..1086138 (+) Length   319 a.a.
NCBI ID   WP_002856368.1    Uniprot ID   -
Organism   Campylobacter jejuni strain CJP19-D445 isolate CFSAN091031     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1067496..1090190 1085179..1086138 within 0


Gene organization within MGE regions


Location: 1067496..1090190
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JG535_RS05450 (JG535_05450) pglI 1067496..1068425 (-) 930 WP_002856262.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  JG535_RS05455 (JG535_05455) pglH 1068418..1069497 (-) 1080 WP_002911763.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  JG535_RS05460 (JG535_05460) pglK 1069494..1071188 (-) 1695 WP_002856016.1 ABC-type lipopolysaccharide transporter PglK -
  JG535_RS05465 (JG535_05465) galE 1071182..1072168 (-) 987 WP_002856461.1 UDP-glucose 4-epimerase GalE -
  JG535_RS05470 (JG535_05470) - 1072226..1073020 (-) 795 WP_002856546.1 3'-5' exonuclease -
  JG535_RS05475 (JG535_05475) waaC 1073086..1074114 (+) 1029 WP_002855693.1 lipopolysaccharide heptosyltransferase I -
  JG535_RS05480 (JG535_05480) htrB 1074104..1074991 (+) 888 WP_002855840.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  JG535_RS05485 (JG535_05485) - 1074988..1076536 (+) 1549 Protein_1068 glycosyltransferase -
  JG535_RS05490 (JG535_05490) - 1076540..1077709 (+) 1170 WP_002852863.1 glycosyltransferase family 2 protein -
  JG535_RS05495 (JG535_05495) - 1077779..1078822 (+) 1044 WP_002912176.1 beta-1,4-N-acetylgalactosaminyltransferase -
  JG535_RS05500 (JG535_05500) - 1078808..1079713 (-) 906 WP_002852740.1 glycosyltransferase family 2 protein -
  JG535_RS05505 (JG535_05505) - 1079802..1080677 (+) 876 WP_002869021.1 alpha-2,3-sialyltransferase -
  JG535_RS05510 (JG535_05510) neuB 1080678..1081718 (+) 1041 WP_002870496.1 N-acetylneuraminate synthase -
  JG535_RS05515 (JG535_05515) neuC 1081715..1082833 (+) 1119 WP_002911676.1 UDP-N-acetylglucosamine 2-epimerase -
  JG535_RS05520 (JG535_05520) - 1082830..1083495 (+) 666 WP_002855897.1 acylneuraminate cytidylyltransferase family protein -
  JG535_RS05525 (JG535_05525) - 1083483..1084316 (+) 834 WP_002855772.1 CatB-related O-acetyltransferase -
  JG535_RS05530 (JG535_05530) waaV 1084308..1085120 (-) 813 WP_002856066.1 glycosyltransferase family 2 protein -
  JG535_RS05535 (JG535_05535) waaF 1085179..1086138 (+) 960 WP_002856368.1 lipopolysaccharide heptosyltransferase II Regulator
  JG535_RS05540 (JG535_05540) gmhA 1086119..1086679 (-) 561 WP_002855785.1 D-sedoheptulose 7-phosphate isomerase -
  JG535_RS05545 (JG535_05545) rfaE1 1086676..1088070 (-) 1395 WP_032581686.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  JG535_RS05550 (JG535_05550) rfaD 1088063..1089016 (-) 954 WP_002856483.1 ADP-glyceromanno-heptose 6-epimerase -
  JG535_RS05555 (JG535_05555) gmhB 1089017..1089577 (-) 561 WP_002911679.1 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  JG535_RS05560 (JG535_05560) - 1089659..1089961 (+) 303 WP_002852762.1 cytochrome c -
  JG535_RS05565 (JG535_05565) ccoS 1089984..1090190 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -

Sequence

Protein


Download         Length: 319 a.a.        Molecular weight: 36719.17 Da        Isoelectric Point: 10.1941

>NTDB_id=522310 JG535_RS05535 WP_002856368.1 1085179..1086138(+) (waaF) [Campylobacter jejuni strain CJP19-D445 isolate CFSAN091031]
MKIFIHLPTWLGDAVMASPALYAIKEHFKDAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQALSLRKELGKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLDIKAHFKDLKLPFKLKFQNPLILKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHLSAVYKVKTVAIFGPTNFTQTSPWQNENAKLVHLDLACMPCMQKTCPLKHHKCMKDLKPQRVIEEARNLLKNSHL

Nucleotide


Download         Length: 960 bp        

>NTDB_id=522310 JG535_RS05535 WP_002856368.1 1085179..1086138(+) (waaF) [Campylobacter jejuni strain CJP19-D445 isolate CFSAN091031]
ATGAAAATTTTTATACACCTTCCAACTTGGCTAGGCGATGCGGTGATGGCTTCACCTGCTTTATACGCTATAAAAGAACA
CTTTAAAGATGCCCAGTTTATCCTTTATGGCTCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCCCGTTATAAACAAGCCCTATCTTTACGCAAAGAACTTGGTAAGATCGATTTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTACATATTCTCAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTGACATAAAAGCTCATTTTAAAGACTTAA
AACTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTATCTTAAAAAATGGCAAAAAAATTCTAGGACTCAACCCTGGCGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAAATTTT
AATCTTTGGCGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGTCCTATGCACCTAAGTGCGGTTTATAAGGTAAAAACCGTAGCTATTTTTGGCCCCACAAATTTTACCCAAACTTCACC
TTGGCAAAATGAAAACGCAAAATTAGTACATTTAGATCTAGCTTGCATGCCTTGTATGCAAAAAACCTGTCCTTTAAAAC
ACCACAAATGCATGAAAGATCTAAAGCCTCAAAGGGTTATAGAAGAGGCTAGAAATTTACTTAAAAACTCTCATCTATGA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(95-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

95.611

100

0.956