Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K5A55_RS18930 Genome accession   NZ_CP081306
Coordinates   3902326..3902766 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain Ec1079     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3897326..3907766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5A55_RS18910 (K5A55_18910) aroP 3898324..3899697 (+) 1374 WP_001347572.1 aromatic amino acid transporter AroP -
  K5A55_RS18915 (K5A55_18915) ampE 3899740..3900594 (-) 855 WP_221582532.1 beta-lactamase regulator AmpE -
  K5A55_RS18920 (K5A55_18920) ampD 3900591..3901142 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K5A55_RS18925 (K5A55_18925) nadC 3901230..3902123 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K5A55_RS18930 (K5A55_18930) pilA 3902326..3902766 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  K5A55_RS18935 (K5A55_18935) pilB 3902776..3904161 (+) 1386 WP_001025174.1 type II secretion system protein GspE Machinery gene
  K5A55_RS18940 (K5A55_18940) hofC 3904151..3905353 (+) 1203 WP_000157236.1 protein transport protein HofC -
  K5A55_RS18945 (K5A55_18945) guaC 3905388..3906431 (-) 1044 WP_001217338.1 GMP reductase -
  K5A55_RS18950 (K5A55_18950) - 3906587..3906631 (-) 45 WP_120795372.1 protein YacM -
  K5A55_RS18955 (K5A55_18955) coaE 3906656..3907276 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=522101 K5A55_RS18930 WP_000360895.1 3902326..3902766(+) (pilA) [Escherichia coli strain Ec1079]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=522101 K5A55_RS18930 WP_000360895.1 3902326..3902766(+) (pilA) [Escherichia coli strain Ec1079]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363