Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K3176_RS25240 Genome accession   NZ_CP081202
Coordinates   5214116..5215468 (-) Length   450 a.a.
NCBI ID   WP_003123870.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain P9W     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5209116..5220468
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K3176_RS25205 (K3176_25205) thiS 5209350..5209550 (-) 201 WP_003084514.1 sulfur carrier protein ThiS -
  K3176_RS25210 (K3176_25210) - 5209653..5210030 (-) 378 WP_003084513.1 DUF423 domain-containing protein -
  K3176_RS25215 (K3176_25215) mtgA 5210067..5210798 (+) 732 WP_003100972.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  K3176_RS25220 (K3176_25220) - 5210803..5211408 (-) 606 WP_003084511.1 hypothetical protein -
  K3176_RS25225 (K3176_25225) rpoH 5211473..5212327 (-) 855 WP_003084510.1 RNA polymerase sigma factor RpoH -
  K3176_RS25230 (K3176_25230) ftsX 5212441..5213448 (-) 1008 WP_003084508.1 permease-like cell division protein FtsX -
  K3176_RS25235 (K3176_25235) ftsE 5213448..5214119 (-) 672 WP_003084505.1 cell division ATP-binding protein FtsE -
  K3176_RS25240 (K3176_25240) pilA 5214116..5215468 (-) 1353 WP_003123870.1 signal recognition particle-docking protein FtsY Machinery gene
  K3176_RS25245 (K3176_25245) - 5215589..5216986 (+) 1398 WP_003084498.1 pitrilysin family protein -
  K3176_RS25250 (K3176_25250) - 5216979..5218466 (+) 1488 WP_003117890.1 pitrilysin family protein -
  K3176_RS25255 (K3176_25255) rsmD 5218466..5219062 (+) 597 WP_003102864.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  K3176_RS25260 (K3176_25260) - 5219065..5219631 (+) 567 WP_023910832.1 hypothetical protein -

Sequence

Protein


Download         Length: 450 a.a.        Molecular weight: 47872.27 Da        Isoelectric Point: 4.5795

>NTDB_id=521894 K3176_RS25240 WP_003123870.1 5214116..5215468(-) (pilA) [Pseudomonas aeruginosa strain P9W]
MFGSNDDKKAPQGGEKKGLFGWWRKKPQAGEQPADQPVEPVSETAAAEQRAPADDVAQSLTEQPGRQQPSAAEPAEPAPV
AEAPLASDEPASAEEHSPRPEAPVAQPEPILAAEPEPEPEPEPEPVAPLAAAPAVSEPATRPGFFARLRQGLSKTSASIG
EGMASLFLGRKEIDDDLLDDIETRLLTADVGVEATTLIVQNLTKRVARKELADSGALYKALQEELASLLRPVEQPLQVDV
AREPYVILVVGVNGVGKTTTIGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNRIPVIAQHTGADSASVIFDAVQ
AAKARGIDVLIADTAGRLHTKDNLMEELKKVRRVIGKLDETAPHEVLLVLDAGTGQNAINQAKQFNLAVELTGLALTKLD
GTAKGGVIFALAKQFGLPIRYIGVGEGIDDLRTFEADAFVQALFAERENA

Nucleotide


Download         Length: 1353 bp        

>NTDB_id=521894 K3176_RS25240 WP_003123870.1 5214116..5215468(-) (pilA) [Pseudomonas aeruginosa strain P9W]
ATGTTTGGTTCCAACGACGACAAGAAGGCCCCGCAGGGCGGGGAAAAGAAAGGTCTGTTCGGCTGGTGGCGGAAAAAGCC
GCAAGCCGGCGAGCAGCCTGCCGATCAGCCTGTCGAGCCGGTCTCCGAGACAGCGGCCGCCGAGCAGCGCGCGCCTGCCG
ATGACGTGGCGCAATCCCTGACAGAACAGCCAGGTCGGCAGCAGCCCTCCGCTGCCGAGCCGGCCGAGCCGGCGCCGGTT
GCCGAAGCACCGCTCGCCAGTGACGAGCCAGCGTCGGCCGAGGAGCATTCGCCCCGGCCCGAGGCGCCCGTCGCGCAACC
CGAGCCTATCCTGGCTGCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGTTGCGCCGCTGGCCGCCGCTCCGG
CAGTCAGCGAGCCGGCGACCAGGCCGGGCTTCTTCGCCCGCCTCCGGCAGGGGCTGTCCAAGACCAGCGCCAGCATTGGC
GAAGGCATGGCCAGCCTGTTCCTCGGTCGCAAGGAAATCGACGACGACCTGCTCGACGACATCGAGACGCGCCTGCTGAC
CGCCGACGTCGGCGTCGAGGCCACCACCCTGATCGTGCAGAACCTGACCAAGCGCGTGGCGCGCAAGGAGCTGGCCGACA
GCGGCGCGCTGTACAAGGCGCTGCAGGAAGAGCTGGCCAGCCTGCTGCGCCCGGTGGAGCAGCCGTTGCAGGTCGACGTC
GCGCGCGAGCCCTATGTGATCCTGGTGGTCGGGGTGAACGGCGTGGGCAAGACCACCACCATCGGCAAGCTGGCGAAGAA
GCTGCAACTCGAGGGCAAGAAGGTCATGCTGGCCGCCGGCGACACCTTCCGCGCCGCCGCCGTGGAGCAGTTGCAGGTCT
GGGGCGAGCGCAACCGCATCCCGGTAATCGCCCAGCACACCGGCGCCGACTCCGCCTCGGTGATCTTCGACGCGGTGCAG
GCCGCCAAGGCCCGCGGCATCGACGTGCTGATCGCCGATACCGCCGGACGCCTGCATACCAAGGACAACCTGATGGAGGA
GCTGAAGAAGGTGCGCCGGGTGATCGGCAAGCTGGACGAGACGGCGCCCCATGAGGTCCTGCTGGTGCTCGACGCCGGCA
CCGGGCAGAACGCCATCAATCAGGCCAAGCAGTTCAACCTCGCGGTGGAGCTGACCGGCCTGGCCCTGACCAAACTGGAC
GGCACCGCCAAGGGCGGGGTGATCTTCGCCCTGGCCAAGCAGTTCGGCCTGCCGATCCGCTATATCGGTGTCGGCGAGGG
GATCGACGATCTCCGTACCTTCGAGGCCGACGCCTTCGTCCAGGCACTCTTCGCAGAGCGGGAGAACGCATGA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(245-444)

SRP54-type protein, helical bundle domain (SRP54_N)

(160-225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.454

96

0.456