Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K4F91_RS19520 Genome accession   NZ_CP081192
Coordinates   4022377..4022817 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain EC1722-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4017377..4027817
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K4F91_RS19500 (K4F91_19500) aroP 4018375..4019748 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  K4F91_RS19505 (K4F91_19505) ampE 4019791..4020645 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  K4F91_RS19510 (K4F91_19510) ampD 4020642..4021193 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K4F91_RS19515 (K4F91_19515) nadC 4021281..4022174 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K4F91_RS19520 (K4F91_19520) pilA 4022377..4022817 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  K4F91_RS19525 (K4F91_19525) pilB 4022827..4024212 (+) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  K4F91_RS19530 (K4F91_19530) hofC 4024202..4025404 (+) 1203 WP_000157227.1 protein transport protein HofC -
  K4F91_RS19535 (K4F91_19535) guaC 4025439..4026482 (-) 1044 WP_001217342.1 GMP reductase -
  K4F91_RS19540 (K4F91_19540) - 4026638..4026682 (-) 45 WP_176350099.1 hypothetical protein -
  K4F91_RS19545 (K4F91_19545) coaE 4026707..4027327 (+) 621 WP_020218810.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=521811 K4F91_RS19520 WP_000360895.1 4022377..4022817(+) (pilA) [Escherichia coli strain EC1722-1]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=521811 K4F91_RS19520 WP_000360895.1 4022377..4022817(+) (pilA) [Escherichia coli strain EC1722-1]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363