Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   K3F56_RS18355 Genome accession   NZ_CP080620
Coordinates   3868675..3869352 (-) Length   225 a.a.
NCBI ID   WP_000178154.1    Uniprot ID   P25960
Organism   Escherichia coli strain TOP10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3863675..3874352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K3F56_RS18330 (K3F56_18330) ytfH 3864098..3864478 (+) 381 WP_000084622.1 helix-turn-helix domain-containing protein -
  K3F56_RS18335 (K3F56_18335) - 3864661..3864750 (+) 90 Protein_3622 hypothetical protein -
  K3F56_RS18340 (K3F56_18340) chiA 3865042..3867735 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  K3F56_RS18345 (K3F56_18345) bfd 3867904..3868098 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  K3F56_RS18350 (K3F56_18350) bfr 3868170..3868646 (+) 477 WP_000675504.1 bacterioferritin -
  K3F56_RS18355 (K3F56_18355) pilD 3868675..3869352 (-) 678 WP_000178154.1 prepilin peptidase GspO Machinery gene
  K3F56_RS18360 (K3F56_18360) gspM 3869352..3869813 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  K3F56_RS18365 (K3F56_18365) gspL 3869810..3870973 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  K3F56_RS18370 (K3F56_18370) gspK 3870988..3871971 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  K3F56_RS18375 (K3F56_18375) gspJ 3871964..3872551 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  K3F56_RS18380 (K3F56_18380) gspI 3872544..3872921 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  K3F56_RS18385 (K3F56_18385) gspH 3872918..3873427 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  K3F56_RS18390 (K3F56_18390) gspG 3873435..3873872 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24956.82 Da        Isoelectric Point: 7.6353

>NTDB_id=521172 K3F56_RS18355 WP_000178154.1 3868675..3869352(-) (pilD) [Escherichia coli strain TOP10]
MTMLLPLFILVGFIADYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=521172 K3F56_RS18355 WP_000178154.1 3868675..3869352(-) (pilD) [Escherichia coli strain TOP10]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P25960

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378