Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A225_RS24325 Genome accession   NC_018106
Coordinates   5220571..5221551 (-) Length   326 a.a.
NCBI ID   WP_014839535.1    Uniprot ID   A0A249WJI3
Organism   Klebsiella michiganensis E718     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5215571..5226551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A225_RS24295 (A225_4972) endA 5215860..5216567 (+) 708 WP_014226832.1 deoxyribonuclease I -
  A225_RS24300 (A225_4973) rsmE 5216659..5217390 (+) 732 WP_014226833.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  A225_RS24305 (A225_4974) gshB 5217411..5218358 (+) 948 WP_014226834.1 glutathione synthase -
  A225_RS24310 (A225_4975) - 5218535..5219098 (+) 564 WP_014226835.1 YqgE/AlgH family protein -
  A225_RS24315 (A225_4976) ruvX 5219098..5219514 (+) 417 WP_014226836.1 Holliday junction resolvase RuvX -
  A225_RS24320 (A225_4977) - 5219570..5220222 (-) 653 Protein_4889 LuxR C-terminal-related transcriptional regulator -
  A225_RS24325 (A225_4979) pilT 5220571..5221551 (-) 981 WP_014839535.1 type IV pilus twitching motility protein PilT Machinery gene
  A225_RS24330 (A225_4980) - 5221568..5222272 (+) 705 WP_014226839.1 YggS family pyridoxal phosphate-dependent enzyme -
  A225_RS24335 (A225_4981) - 5222290..5222856 (+) 567 WP_004105714.1 YggT family protein -
  A225_RS24340 (A225_4982) yggU 5222853..5223143 (+) 291 WP_004854155.1 DUF167 family protein YggU -
  A225_RS24345 (A225_4983) - 5223156..5223749 (+) 594 WP_004854157.1 XTP/dITP diphosphatase -
  A225_RS24350 (A225_4984) hemW 5223742..5224878 (+) 1137 WP_014839536.1 radical SAM family heme chaperone HemW -
  A225_RS24355 (A225_4985) - 5225183..5226169 (+) 987 WP_014839537.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35210.42 Da        Isoelectric Point: 6.7582

>NTDB_id=52099 A225_RS24325 WP_014839535.1 5220571..5221551(-) (pilT) [Klebsiella michiganensis E718]
MELEEIVALSVKHNVSDLHLCNASTPRWRRQGKLEPAPFTSPDIGKILADWLNAEQLAQWQACGQVDFALAPAGGPRLRA
SAFVHNRGLSLALRLLAETCPQLADLGAPAALTELLNEESGLILVTGATGSGKSTTLAAMVGYLNQRLNGHILTLEDPVE
FIHRSERCLIQQREIGRHCPSFAAALRAALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAQAVERLVDV
FPAQEKEQVRSQLAGSLCAVLAQKLFPDTAGGRVALYELLVNTPAVANLIREGKTHQLPGVMQTGQQFGMQTFTQSFQQR
TAAGLL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=52099 A225_RS24325 WP_014839535.1 5220571..5221551(-) (pilT) [Klebsiella michiganensis E718]
ATGGAACTGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAATGCTTCAACGCCGCG
CTGGCGTCGCCAGGGCAAGCTGGAGCCTGCGCCTTTTACCTCTCCGGATATTGGCAAGATACTCGCTGATTGGCTCAACG
CCGAGCAGCTCGCCCAGTGGCAGGCCTGCGGGCAGGTTGATTTCGCGCTGGCTCCGGCCGGCGGCCCGCGCCTGCGCGCC
AGCGCATTTGTCCACAACCGCGGCCTGTCGCTGGCCCTGCGTCTGCTGGCAGAAACCTGCCCGCAGCTGGCGGATCTCGG
CGCGCCGGCCGCCTTAACGGAGCTGCTCAATGAGGAGAGCGGGCTTATCCTGGTCACGGGCGCCACCGGTAGCGGGAAAT
CCACCACCCTGGCGGCGATGGTGGGCTATCTTAATCAGCGCCTCAATGGCCATATCCTGACGCTGGAAGATCCGGTTGAG
TTTATTCACCGTAGCGAGCGCTGCCTGATCCAGCAGCGGGAAATCGGTCGCCACTGCCCCTCATTTGCCGCGGCCCTGCG
CGCTGCGCTGCGTCAGGATCCGGATGTCATTCTGCTAGGGGAACTGCGTGACAGCGAAACGATCCGCCTGGCATTGACCG
CGGCGGAGACCGGACATCTGGTTATGGCGACGCTGCATACGCGCGGCGCGGCACAGGCGGTTGAAAGGCTGGTGGATGTT
TTTCCTGCCCAGGAGAAAGAGCAGGTTCGTAGCCAGCTGGCGGGAAGTTTATGCGCGGTGCTGGCACAAAAATTGTTTCC
GGATACTGCTGGCGGAAGAGTTGCGCTATACGAGCTTTTGGTTAATACCCCGGCGGTGGCGAATTTGATCCGCGAGGGGA
AAACGCATCAGCTGCCCGGCGTGATGCAAACCGGGCAGCAATTCGGGATGCAAACTTTTACGCAAAGCTTTCAGCAACGT
ACGGCTGCGGGTCTTTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249WJI3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.848

100

0.503
  pilT Vibrio cholerae strain A1552

49.848

100

0.503
  pilT Neisseria meningitidis 8013

48.182

100

0.488
  pilT Neisseria gonorrhoeae MS11

47.879

100

0.485
  pilT Legionella pneumophila strain Lp02

48.113

97.546

0.469
  pilT Legionella pneumophila strain ERS1305867

48.113

97.546

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.201

100

0.466
  pilT Acinetobacter baumannii strain A118

45.455

100

0.46
  pilT Acinetobacter baumannii D1279779

45.455

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.593

100

0.46
  pilT Acinetobacter nosocomialis M2

45.152

100

0.457
  pilT Acinetobacter baylyi ADP1

44.985

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.479

97.239

0.433
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.414
  pilU Vibrio cholerae strain A1552

40.683

98.773

0.402
  pilU Acinetobacter baylyi ADP1

38.39

99.08

0.38

Multiple sequence alignment