Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   K0H55_RS02215 Genome accession   NZ_CP080370
Coordinates   473310..473987 (-) Length   225 a.a.
NCBI ID   WP_001377644.1    Uniprot ID   -
Organism   Escherichia coli strain PK5086     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 468310..478987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0H55_RS02200 (K0H55_02200) chiA 469677..472370 (+) 2694 WP_001377643.1 bifunctional chitinase/lysozyme -
  K0H55_RS02205 (K0H55_02205) bfd 472539..472733 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  K0H55_RS02210 (K0H55_02210) bfr 472805..473281 (+) 477 WP_000675504.1 bacterioferritin -
  K0H55_RS02215 (K0H55_02215) pilD 473310..473987 (-) 678 WP_001377644.1 prepilin peptidase GspO Machinery gene
  K0H55_RS02220 (K0H55_02220) gspM 473987..474448 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  K0H55_RS02225 (K0H55_02225) gspL 474445..475608 (-) 1164 WP_001115230.1 type II secretion system protein GspL -
  K0H55_RS02230 (K0H55_02230) gspK 475623..476606 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  K0H55_RS02235 (K0H55_02235) gspJ 476599..477186 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  K0H55_RS02240 (K0H55_02240) gspI 477179..477556 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  K0H55_RS02245 (K0H55_02245) gspH 477553..478062 (-) 510 WP_001076048.1 type II secretion system minor pseudopilin GspH -
  K0H55_RS02250 (K0H55_02250) gspG 478070..478507 (-) 438 WP_001202880.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24838.75 Da        Isoelectric Point: 7.8986

>NTDB_id=519447 K0H55_RS02215 WP_001377644.1 473310..473987(-) (pilD) [Escherichia coli strain PK5086]
MTMLLPLFILVGFIAGCFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=519447 K0H55_RS02215 WP_001377644.1 473310..473987(-) (pilD) [Escherichia coli strain PK5086]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTGTTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.382
  pilD Vibrio cholerae strain A1552

35.745

100

0.373