Detailed information NTG0519332

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: JFU10_RS11085
• Genome accession: NZ_CP066337
• Coordinates: 2284781..2286160 (+)
• Length: 459 a.a.
• NCBI ID: WP_014416742.1
• Uniprot ID: I2C0L1
• Organism: Bacillus velezensis strain Mr12
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2279781..2291160

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JFU10_RS11065	ctsR	2280132..2280596 (+)	465	WP_198863446.1	transcriptional regulator CtsR	-
JFU10_RS11070	-	2280610..2281167 (+)	558	WP_003156397.1	UvrB/UvrC motif-containing protein	-
JFU10_RS11075	-	2281167..2282258 (+)	1092	WP_003156398.1	protein arginine kinase	-
JFU10_RS11080	clpC	2282255..2284687 (+)	2433	WP_007410388.1	ATP-dependent protease ATP-binding subunit ClpC	Regulator
JFU10_RS11085	radA	2284781..2286160 (+)	1380	WP_014416742.1	DNA repair protein RadA	Machinery gene
JFU10_RS11090	disA	2286164..2287246 (+)	1083	WP_003156401.1	DNA integrity scanning diadenylate cyclase DisA	-
JFU10_RS11095	-	2287360..2288460 (+)	1101	WP_003156403.1	PIN/TRAM domain-containing protein	-
JFU10_RS11100	ispD	2288473..2289171 (+)	699	WP_071392202.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
JFU10_RS11105	ispF	2289164..2289640 (+)	477	WP_003156407.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-

Sequence

Protein

• Length: 459 a.a.
• Molecular weight: 49547.98 Da
• Isoelectric Point: 7.1760

>NTDB_id=519332 JFU10_RS11085 WP_014416742.1 2284781..2286160(+) (radA) [Bacillus velezensis strain Mr12]
MAKSKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVHTKLEEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLADTAGSVLYISGEESVKQTKLRADRLGINSQMLHVLSETDMEYIS
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGASGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPEANADGWTIPKGIEVVGVANVAEALRTSLGGS

Nucleotide

• Length: 1380 bp

>NTDB_id=519332 JFU10_RS11085 WP_014416742.1 2284781..2286160(+) (radA) [Bacillus velezensis strain Mr12]
ATGGCTAAATCAAAAACAAAATTCATCTGCCACTCATGCGGTTATGAATCCGCAAAGTGGATGGGAAAGTGCCCGGGCTG
CGGGGCTTGGAATACAATGGTGGAAGAAACCATAAAAAAAGCGCCGGCGAATCGGCGGGCGGCTTTCTCCCACTCCGTTC
AAACCGTCCAGAAACCATCACCCATTACATCAATTGAAACATCAGAAGAACCCCGCGTTCACACAAAGCTCGAAGAATTC
AACAGAGTGCTTGGCGGCGGCGTTGTGAAAGGCTCGTTAGTATTGATCGGCGGCGATCCCGGGATCGGAAAATCAACGCT
CCTTCTCCAAGTATCCGCACAATTAGCAGATACGGCCGGCAGCGTGCTTTACATTTCAGGGGAAGAATCCGTGAAGCAAA
CGAAGCTGCGCGCCGACAGACTTGGAATCAACAGCCAAATGTTACATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAAACCCGCATTTGTTGTCGTAGACTCCATTCAGACCGTTTACCAAAGTGATATCACCTCAGC
TCCCGGCAGTGTATCGCAAGTAAGAGAGTGTACGGCCGAATTGATGAAAATTGCGAAAACAAACGGCATACCTATTTTTA
TCGTCGGACACGTCACAAAAGAAGGATCGATCGCAGGGCCGAGATTGCTTGAGCACATGGTCGATACCGTTCTATACTTT
GAAGGAGAACGGCACCATACATTCCGTATTTTGCGGGCCGTAAAAAACCGTTTCGGCTCAACAAATGAAATGGGCATCTT
TGAAATGCGGGAAGAGGGGCTTACGGAAGTGCTGAACCCTTCTGAAATTTTCTTGGAAGAGCGTTCTGCCGGGGCATCGG
GCTCGAGTATCGTTGCGTCAATGGAAGGAACGAGACCGATTCTGGTTGAAATCCAGGCGCTCATATCACCGACCAGTTTT
GGAAACCCGCGCCGCATGGCTACAGGCATTGACCACAACAGGGTATCGTTAATTATGGCTGTGCTTGAGAAGCGGGTGGG
TCTTTTGCTTCAAAATCAAGATGCATATTTAAAAGTCGCCGGCGGGGTCAAACTGGATGAACCGGCGATTGATTTGGCAG
TAGCCGTCAGCATTGCCTCAAGCTTCAGAGATACACCGCCGAATCCCGCAGATTGCTTTATCGGTGAAGTGGGACTGACA
GGAGAAGTCCGCAGGGTGTCAAGAATCGAACAGCGCGTAAAAGAAGCGGCGAAACTCGGCTTCAAGCGCATGATCATACC
TGAGGCGAATGCAGACGGGTGGACAATACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCTGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (8-35)

• Subunit ChlI of Mg-chelatase (ChlI) (364-430)

• AAA domain (AAA_25) (73-222)

3D structure

• AlphaFold DB: I2C0L1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Bacillus subtilis subsp. subtilis str. 168	93.45	99.782	0.932
radA	Streptococcus pneumoniae Rx1	63.797	98.693	0.63
radA	Streptococcus pneumoniae D39	63.797	98.693	0.63
radA	Streptococcus pneumoniae R6	63.797	98.693	0.63
radA	Streptococcus pneumoniae TIGR4	63.797	98.693	0.63
radA	Streptococcus mitis NCTC 12261	63.797	98.693	0.63
radA	Streptococcus mitis SK321	63.576	98.693	0.627