Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   JFU10_RS00260 Genome accession   NZ_CP066337
Coordinates   107760..108194 (+) Length   144 a.a.
NCBI ID   WP_007611576.1    Uniprot ID   A0A172XIP3
Organism   Bacillus velezensis strain Mr12     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 102760..113194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JFU10_RS00255 - 106260..107471 (-) 1212 WP_166706274.1 cytochrome P450 family protein -
  JFU10_RS00260 nucA/comI 107760..108194 (+) 435 WP_007611576.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  JFU10_RS00265 - 108293..109009 (+) 717 WP_015417517.1 YoaK family protein -
  JFU10_RS00270 - 109043..109405 (-) 363 WP_007410383.1 hypothetical protein -
  JFU10_RS00275 - 109598..110926 (-) 1329 WP_071391804.1 S8 family peptidase -
  JFU10_RS00280 - 111105..111338 (+) 234 WP_015239897.1 hypothetical protein -
  JFU10_RS00285 - 111594..112301 (+) 708 WP_003154076.1 poly-gamma-glutamate hydrolase family protein -
  JFU10_RS00290 - 112361..112813 (+) 453 WP_014305039.1 OsmC family protein -
  JFU10_RS00295 - 112827..113180 (-) 354 WP_003154073.1 DMT family transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15528.47 Da        Isoelectric Point: 7.2418

>NTDB_id=519289 JFU10_RS00260 WP_007611576.1 107760..108194(+) (nucA/comI) [Bacillus velezensis strain Mr12]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=519289 JFU10_RS00260 WP_007611576.1 107760..108194(+) (nucA/comI) [Bacillus velezensis strain Mr12]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCAGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGCTATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172XIP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486