Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   I9H07_RS20470 Genome accession   NZ_CP066265
Coordinates   4590740..4592107 (-) Length   455 a.a.
NCBI ID   WP_003381736.1    Uniprot ID   Q0EDX1
Organism   Pseudomonas syringae strain PA-2-9E     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4585740..4597107
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9H07_RS20450 (I9H07_20330) yjiA 4586708..4587685 (-) 978 WP_236424134.1 GTPase -
  I9H07_RS20455 (I9H07_20335) - 4587781..4587978 (-) 198 WP_024674876.1 YbdD/YjiX family protein -
  I9H07_RS20460 (I9H07_20340) - 4588006..4590081 (-) 2076 WP_024674877.1 carbon starvation CstA family protein -
  I9H07_RS20465 (I9H07_20345) - 4590365..4590733 (+) 369 WP_024674878.1 PilZ domain-containing protein -
  I9H07_RS20470 (I9H07_20350) radA 4590740..4592107 (-) 1368 WP_003381736.1 DNA repair protein RadA Machinery gene
  I9H07_RS20475 (I9H07_20355) mscL 4592302..4592748 (+) 447 WP_024674879.1 large-conductance mechanosensitive channel protein MscL -
  I9H07_RS20480 (I9H07_20360) - 4592869..4593645 (-) 777 WP_236424136.1 ferredoxin--NADP reductase -
  I9H07_RS20485 (I9H07_20365) - 4593998..4594231 (+) 234 WP_024674881.1 helix-turn-helix transcriptional regulator -
  I9H07_RS20490 (I9H07_20370) - 4594360..4595484 (+) 1125 WP_236424138.1 methyltransferase -
  I9H07_RS20495 (I9H07_20375) - 4595572..4595739 (-) 168 WP_002555239.1 DUF2474 domain-containing protein -
  I9H07_RS20500 (I9H07_20380) cydB 4595761..4596768 (-) 1008 WP_003419100.1 cytochrome d ubiquinol oxidase subunit II -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48839.40 Da        Isoelectric Point: 7.1315

>NTDB_id=519012 I9H07_RS20470 WP_003381736.1 4590740..4592107(-) (radA) [Pseudomonas syringae strain PA-2-9E]
MAKAKRLYGCTECGATFPKWAGQCADCGAWNTLVETMIESGAAAPPSGRTGWTGSQAQIRTLAEVSVEEIPRFSTNSAEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAQRMPALYVTGEESQQQVAMRARRLGLPQDKLRVMTETCIETIIA
TAKVEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHMS
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPPGLQIIAVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=519012 I9H07_RS20470 WP_003381736.1 4590740..4592107(-) (radA) [Pseudomonas syringae strain PA-2-9E]
ATGGCCAAGGCCAAACGCTTGTACGGCTGCACCGAGTGCGGCGCGACATTTCCCAAGTGGGCGGGCCAGTGCGCCGATTG
CGGCGCCTGGAACACACTGGTCGAAACCATGATTGAAAGCGGTGCCGCCGCGCCGCCCAGCGGACGCACTGGCTGGACCG
GCTCACAGGCGCAAATCAGGACCCTGGCGGAAGTCAGCGTCGAAGAAATCCCGCGGTTCTCCACCAATTCCGCCGAACTG
GACCGGGTGCTCGGCGGCGGTCTGGTGGACGGCTCGGTGGTGCTGATCGGCGGTGATCCTGGCATCGGCAAGTCGACCAT
CCTGCTGCAGACGCTGTGCAATATCGCCCAGCGCATGCCGGCTCTGTATGTCACGGGTGAGGAGTCCCAGCAGCAAGTCG
CCATGCGCGCCCGTCGTCTTGGGCTGCCGCAGGACAAACTGCGGGTCATGACCGAAACCTGCATCGAGACCATCATCGCC
ACGGCCAAGGTCGAGAAGCCCAAGGTAATGGTGATCGACTCGATCCAGACCATCTTCACCGAACAGTTGCAGTCGGCACC
GGGCGGCGTGTCGCAGGTTCGTGAAAGCGCCGCGCTGCTAGTGCGCTACGCGAAACAGAGCGGCACGGCGATCTTCCTGG
TGGGCCATGTCACCAAGGAAGGCGCACTGGCCGGTCCACGCGTGCTGGAGCACATGGTCGACACCGTGCTGTATTTCGAA
GGTGAGTCGGATGGGCGCCTGCGTCTGTTGCGGGCGGTGAAGAACCGCTTCGGCGCGGTTAACGAGCTAGGCGTGTTCGG
CATGACCGACAAAGGCCTCAAGGAAGTCTCCAACCCGTCGGCGATTTTCCTGACCCGTGCGCAGGAAGAAGTGCCGGGCA
GTGTGGTCATGGCCACTTGGGAAGGTACGCGCCCCATGCTGGTGGAAGTTCAGGCGCTGGTGGATGACAGCCACATGTCC
AACCCGCGCCGGGTGACGCTGGGCCTGGATCAGAACCGTCTGGCGATGCTCCTGGCAGTGCTGCACCGGCATGGTGGCAT
TCCGACCCACGATCAGGACGTGTTTCTCAACGTGGTCGGTGGGGTGAAGGTGCTGGAAACCGCCTCGGATCTGGCGTTGA
TGGCTGCGGTCATGTCCAGCCTGCGCAATCGTCCGTTGCCGCATGATTTGCTGGTGTTCGGCGAAGTGGGCCTGTCTGGC
GAAGTCAGGCCGGTGCCGAGCGGTCAGGAGCGCCTGAAAGAGGCCGCCAAGCATGGCTTCAAGCGGGCCATCGTGCCGAA
AGGCAATGCGCCGAAGGAAGCGCCTCCCGGCTTGCAGATCATTGCTGTCACGCGCCTGGAGCAGGCACTCGACGCGCTGT
TTGAATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)

AAA domain (AAA_25)

(73-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0EDX1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.123

100

0.492
  radA Streptococcus pneumoniae Rx1

46.739

100

0.473
  radA Streptococcus pneumoniae R6

46.739

100

0.473
  radA Streptococcus pneumoniae TIGR4

46.739

100

0.473
  radA Streptococcus pneumoniae D39

46.739

100

0.473
  radA Streptococcus mitis SK321

46.93

100

0.47
  radA Streptococcus mitis NCTC 12261

46.711

100

0.468