Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   I9H07_RS20255 Genome accession   NZ_CP066265
Coordinates   4540532..4542124 (+) Length   530 a.a.
NCBI ID   WP_236424935.1    Uniprot ID   -
Organism   Pseudomonas syringae strain PA-2-9E     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 4535532..4547124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9H07_RS20235 (I9H07_20115) pgeF 4537324..4538052 (-) 729 WP_236424933.1 peptidoglycan editing factor PgeF -
  I9H07_RS20240 (I9H07_20120) rluD 4538049..4539011 (-) 963 WP_005895286.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  I9H07_RS20245 (I9H07_20125) - 4539161..4540183 (+) 1023 WP_024672959.1 outer membrane protein assembly factor BamD -
  I9H07_RS20250 (I9H07_20130) - 4540303..4540542 (+) 240 WP_032606207.1 PP0621 family protein -
  I9H07_RS20255 (I9H07_20135) pilS 4540532..4542124 (+) 1593 WP_236424935.1 ATP-binding protein Regulator
  I9H07_RS20260 (I9H07_20140) pilR 4542127..4543467 (+) 1341 WP_024672957.1 sigma-54 dependent transcriptional regulator Regulator
  I9H07_RS20265 (I9H07_20145) - 4544027..4544644 (+) 618 WP_058824392.1 TetR/AcrR family transcriptional regulator -
  I9H07_RS20270 (I9H07_20150) - 4544726..4545841 (+) 1116 WP_058824393.1 efflux RND transporter periplasmic adaptor subunit -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58382.53 Da        Isoelectric Point: 7.0948

>NTDB_id=519010 I9H07_RS20255 WP_236424935.1 4540532..4542124(+) (pilS) [Pseudomonas syringae strain PA-2-9E]
MTAETPHLASPQAQRILRLYHLYRLTIGVVLVLLISSSLETELLQLANPNLFRSGCWLYLVFNILVVVLLERPTRQAHLF
SLAMVDAIMLSGLFYAAGGPSSGIGNLLIASVAISNVLLRGRIGLLIAAVSAIGIIYLTFYISFGTPSVSNDYVQAGSLG
ALCFAAALLVQALTARMQTSEVLAEQRAADVNSLEELNALILQRMRTGILVLDARRQVLLANQGALALLGHERLVGQVID
AYSPQLVERLRQWLINPIMLPRSISSSAIGPELQPSFVALNRGDQRQTLIFLEDLSQIAHQAQQLKLAALGRLTAGIAHE
IRNPLGAVSHAAQLLNESEALSAPDRRLGQIIHDQSQRIDRVIENVLQLSRRREAQPQLLDLKVWLDAFVTDFRNCALPN
QLLHVEIHSGTLTTRMDAEQLTQVVTNLLQNGLRYSSKQHTLAQVWLKLHHDLNSDLPILEVLDDGPGVSEPHLSKLFEP
FFTTESKGTGLGLYLSRELCESNQAHLEYAHRPEGGSCLRITFAHPFKTS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=519010 I9H07_RS20255 WP_236424935.1 4540532..4542124(+) (pilS) [Pseudomonas syringae strain PA-2-9E]
GTGACCGCTGAGACGCCCCACCTTGCCAGCCCACAGGCGCAGCGGATTCTGCGCCTGTATCACCTTTATCGACTGACCAT
CGGCGTCGTACTGGTGCTGCTGATCTCCAGCAGCCTGGAAACCGAACTGTTGCAACTGGCCAATCCGAACCTGTTTCGCT
CGGGTTGCTGGCTGTACCTGGTGTTCAATATTCTGGTCGTGGTGTTGCTGGAACGGCCTACACGCCAGGCGCATCTGTTC
AGCCTGGCGATGGTCGACGCCATCATGCTGTCCGGTCTGTTCTACGCGGCGGGCGGCCCGTCCAGCGGCATTGGCAACCT
GCTGATCGCCTCGGTGGCCATCAGCAATGTGCTGCTGCGCGGCCGCATCGGCCTGTTGATTGCCGCCGTGTCTGCCATCG
GCATCATTTACCTGACGTTCTACATCAGCTTCGGCACGCCTTCGGTGTCAAACGACTACGTGCAGGCCGGTTCTCTGGGG
GCACTGTGCTTCGCCGCTGCGCTGCTGGTGCAGGCGCTGACCGCGCGCATGCAGACCAGCGAAGTGCTTGCCGAACAGCG
GGCAGCGGACGTCAACAGCCTGGAAGAACTCAACGCTCTGATCCTGCAACGCATGCGCACCGGCATTCTGGTACTGGACG
CCCGCCGTCAGGTGCTCCTGGCCAATCAGGGCGCACTGGCTTTGCTTGGCCACGAGCGGCTGGTCGGTCAGGTTATCGAC
GCTTACTCGCCGCAGCTGGTGGAGCGCCTGCGTCAGTGGCTGATCAACCCGATCATGTTGCCACGCAGCATCAGCTCATC
TGCAATCGGGCCTGAACTGCAACCCAGCTTTGTCGCACTCAATCGTGGCGATCAGCGCCAGACGCTGATTTTTCTCGAAG
ATCTCTCGCAAATCGCCCATCAGGCGCAACAACTCAAGCTCGCCGCGCTGGGGCGTCTGACTGCAGGCATTGCTCACGAA
ATACGCAACCCGCTGGGTGCGGTGAGCCATGCGGCGCAGTTGCTGAACGAGTCGGAAGCGCTCAGCGCGCCGGACCGTCG
TTTGGGGCAGATCATTCACGATCAGTCGCAGCGCATCGACCGGGTGATCGAAAACGTCTTGCAGCTTTCCCGGCGACGCG
AGGCGCAACCGCAGCTGCTGGACCTGAAAGTCTGGCTGGACGCCTTTGTCACCGACTTCCGCAATTGCGCCCTGCCCAAC
CAGCTGCTTCATGTGGAGATCCATTCCGGCACGCTGACCACCCGCATGGACGCGGAGCAACTGACACAGGTGGTGACCAA
TCTGTTGCAGAACGGCCTGCGCTACAGCAGCAAGCAGCACACGTTGGCGCAGGTCTGGCTGAAGCTGCATCATGACCTGA
ACAGCGACCTGCCCATTCTGGAAGTGCTGGATGATGGGCCGGGGGTCAGCGAGCCGCATTTGAGCAAGCTGTTCGAGCCG
TTCTTTACCACCGAAAGCAAAGGCACCGGTCTGGGGCTATACCTTTCCCGAGAGTTATGCGAAAGCAATCAGGCGCACCT
GGAATACGCGCACCGACCAGAAGGCGGCAGTTGTTTGCGCATCACCTTCGCCCACCCGTTCAAAACGAGCTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

62.147

100

0.623