Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   I9H08_RS01615 Genome accession   NZ_CP066263
Coordinates   352356..354575 (-) Length   739 a.a.
NCBI ID   WP_024660643.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. syringae strain Pss9644     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 347356..359575
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9H08_RS01595 (I9H08_01590) hslU 347473..348810 (-) 1338 WP_003316641.1 ATP-dependent protease ATPase subunit HslU -
  I9H08_RS01600 (I9H08_01595) hslV 348877..349407 (-) 531 WP_003392345.1 ATP-dependent protease subunit HslV -
  I9H08_RS01605 (I9H08_01600) - 349822..350517 (-) 696 WP_003403056.1 SPOR domain-containing protein -
  I9H08_RS01610 (I9H08_01605) argS 350519..352258 (-) 1740 WP_003403053.1 arginine--tRNA ligase -
  I9H08_RS01615 (I9H08_01610) priA 352356..354575 (-) 2220 WP_024660643.1 primosomal protein N' Machinery gene
  I9H08_RS01620 (I9H08_01615) rpmE 354745..354966 (+) 222 WP_003403048.1 50S ribosomal protein L31 -
  I9H08_RS01625 (I9H08_01620) - 354980..355783 (+) 804 WP_024639375.1 thermonuclease family protein -
  I9H08_RS01630 (I9H08_01625) - 355965..357233 (+) 1269 WP_011266374.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81928.23 Da        Isoelectric Point: 8.5469

>NTDB_id=518960 I9H08_RS01615 WP_024660643.1 352356..354575(-) (priA) [Pseudomonas syringae pv. syringae strain Pss9644]
MPDAILRLALPSPLRRLFDYRAPAGVSRSALQPGMRLRVPFGRREMIGILVEVVDHSEVPADKLKPAIALLDSEAPLPPA
LFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAESRQERFWHVAPGASVDDPRIARAPKQREALTTLAQHPHGVAHQLL
SKLMLNRDSLNLLLAKELVYVEVRSHAPSARHEHWLAQPELPLNTEQRAAYEAIRAGFDSFHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALVHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNQRAGGAQQPRFMRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSGCQRCDARMTVHQRSGELRCHHCGYVERVPRQC
PSCGKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFTTIQRGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLIQLTEQGYFAFAEQALSERRSASLPPFSHL
ALLRAEAHKPGQAEAFLDQACSDAEQLVAQMALGGIELLGPVPAPMERRAGRYRAQLLVQSSARAPLHKLLATWLLALEQ
MPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=518960 I9H08_RS01615 WP_024660643.1 352356..354575(-) (priA) [Pseudomonas syringae pv. syringae strain Pss9644]
GTGCCCGACGCCATCCTGCGCCTTGCCCTGCCCTCGCCGCTTCGCCGCCTGTTCGACTACCGCGCCCCTGCGGGCGTGTC
GCGCAGCGCCTTGCAGCCGGGCATGCGTCTGCGAGTGCCGTTCGGACGTCGGGAAATGATCGGCATTCTGGTGGAAGTGG
TCGATCACAGCGAAGTACCCGCCGACAAGCTCAAGCCGGCAATCGCCCTGCTCGACAGCGAAGCGCCGCTGCCGCCCGCG
CTGTTCAAGCTGTGCCTGTGGACCTCGCAGTACTACCAGCACAGCCTCGGAGACACACTGAGCTGGGCCTTGCCGGTGCT
GCTGCGTCAGGGCGAACTGGCCGAAAGTCGCCAGGAGCGCTTCTGGCATGTGGCCCCGGGCGCCAGCGTCGACGATCCGC
GCATCGCCCGCGCGCCCAAGCAGCGCGAAGCCCTGACCACCCTTGCCCAGCACCCGCACGGAGTGGCGCATCAACTGCTC
AGCAAGCTGATGCTTAACAGGGACAGCCTCAACCTGTTGCTGGCCAAGGAGCTGGTGTACGTCGAGGTCCGCAGCCATGC
GCCCAGCGCACGCCATGAACACTGGCTGGCCCAGCCTGAATTGCCACTCAACACCGAACAACGCGCCGCTTACGAAGCGA
TCCGCGCCGGATTCGACAGCTTTCATGCGTTTCTGCTGGCAGGTGTCACCGGCAGCGGCAAGACCGAAGTCTATCTGCAA
CTGATCCGCGAAACCCTCGAAGCCGGCAAGCAGGCGCTGGTGCTGATCCCGGAAATCAACCTCGGCCCGCAGACCCTGGC
GCGCTTCGAGCAGCGCTTCAATGCGCGCATTGCGCTGGTCCACTCGGCGGTCAACGACCGCGAACGGCTGGACGCCTGGC
TGGCGGCTCGCGATGGCGAGGCGGACATCATCATCGGCACGCGTTCGGCGCTGTTCACGCCGATGAAAAACCCCGGCCTG
ATCATCATCGACGAAGAACACGACGGTTCCTATAAACAGCAGGAAGGTCTGCGCTATCACGCCCGCGATCTGGCACTGGT
GCGGGCGCGTCAGGAAAACATTCCGATTGTCCTGGGCTCGGCCACGCCGTCCCTGGAAAGCCTGCACAACGCCTACACCG
GCCGCTATGGCCTGTTGCGCCTGAATCAGCGCGCGGGCGGCGCACAACAGCCACGCTTTATGCGCCTGGACGTCAAGAGC
CGACCGCTGGACAGCGGCATCTCCGGGCCCATGCAGCAGGCCATCGGCCAGACGCTCGCGGCAGGCCAGCAGGTTTTGGT
GTTCCTCAACCGTCGCGGCTTTGCGCCAACTTTGCTGTGCCACGACTGCGGCTGGATGTCCGGCTGCCAACGCTGTGATG
CACGCATGACCGTGCACCAGCGCTCGGGCGAACTGCGCTGCCATCACTGCGGGTATGTCGAGCGCGTACCGCGTCAGTGC
CCGTCGTGCGGCAAGGTGGATTTGCGCCCGGTAGGCGCCGGCACCGAGCGCGCCGAAGAGCGCCTGGCGATTCTGTTTCC
GGACTACCCCGTGCTGCGTGTCGATCGCGACAGCACGTCGCGCAAGGACGCCATGAACCAGCTGTTCACGACGATCCAGC
GCGGTCAGCCGTGCATTCTGGTCGGCACGCAAATGCTCGCCAAGGGCCATCACTTTCCCCGGGTCACGCTGGTCTCGATT
CTGGACGCCGACGGCGGGCTGTTTTCCGGCGACTTCCGCGCCAGCGAGCGCATGGCGCAACTGATCGTGCAGGTGGCAGG
CCGTGCCGGGCGCGCCGAAGAGCCTGGCAAGGTGATCATCCAGACCCACCTGGCCGACCATCCGTTGCTGATCCAGCTGA
CCGAGCAGGGTTATTTCGCCTTTGCCGAACAAGCCTTGAGCGAGCGCCGTTCAGCGAGTCTGCCACCGTTTTCACACCTT
GCACTGCTGCGCGCCGAAGCGCACAAGCCGGGACAGGCCGAGGCCTTTCTCGATCAGGCCTGCAGCGATGCCGAGCAACT
GGTTGCGCAAATGGCGCTGGGCGGCATAGAGCTGCTGGGCCCGGTTCCGGCCCCGATGGAACGCCGCGCCGGGCGTTATC
GCGCGCAACTGCTGGTGCAGTCCAGCGCCCGCGCGCCACTGCATAAATTGCTTGCCACCTGGCTGCTCGCACTGGAGCAA
ATGCCCAGCGGTCGACAGGTCAGATGGTCGCTGGATGTGGATCCCGTGGATCTTTATTAA

Domains

Predicted by InterproScan.

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

DEAD/DEAH box helicase (DEAD)

(207-367)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.844

100

0.48