Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KZ312_RS25200 Genome accession   NZ_CP080260
Coordinates   5240450..5241946 (-) Length   498 a.a.
NCBI ID   WP_001040619.1    Uniprot ID   -
Organism   Escherichia coli strain 135     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5235450..5246946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZ312_RS25175 (KZ312_25175) panM 5235671..5236054 (+) 384 WP_000778800.1 aspartate 1-decarboxylase autocleavage activator PanM -
  KZ312_RS29590 - 5236095..5236194 (-) 100 Protein_4944 hypothetical protein -
  KZ312_RS25180 (KZ312_25180) livJ 5236254..5237357 (-) 1104 WP_001021986.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  KZ312_RS25185 (KZ312_25185) rpoH 5237629..5238483 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  KZ312_RS25190 (KZ312_25190) ftsX 5238728..5239786 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  KZ312_RS25195 (KZ312_25195) ftsE 5239779..5240447 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  KZ312_RS25200 (KZ312_25200) pilA 5240450..5241946 (-) 1497 WP_001040619.1 signal recognition particle-docking protein FtsY Machinery gene
  KZ312_RS25205 (KZ312_25205) rsmD 5242096..5242692 (+) 597 WP_000743195.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  KZ312_RS25210 (KZ312_25210) yhhL 5242682..5242951 (+) 270 WP_001295207.1 DUF1145 family protein -
  KZ312_RS25215 (KZ312_25215) yhhM 5242954..5243313 (-) 360 WP_000042883.1 DUF2500 domain-containing protein -
  KZ312_RS25220 (KZ312_25220) yhhN 5243454..5244080 (+) 627 WP_000964718.1 lysoplasmalogenase -
  KZ312_RS25225 (KZ312_25225) zntA 5244154..5246352 (+) 2199 WP_000106497.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  KZ312_RS25230 (KZ312_25230) - 5246385..5246693 (-) 309 WP_000385056.1 hypothetical protein -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54668.50 Da        Isoelectric Point: 4.1468

>NTDB_id=518913 KZ312_RS25200 WP_001040619.1 5240450..5241946(-) (pilA) [Escherichia coli strain 135]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVPAQEPAKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVVIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=518913 KZ312_RS25200 WP_001040619.1 5240450..5241946(-) (pilA) [Escherichia coli strain 135]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCCGGCGCAAGAGCCTGCGAAGGCTTCTGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGTTAT
CGAGCGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAACCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAGCAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTAGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAGGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTTCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378