Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   I2453_RS06280 Genome accession   NZ_CP066242
Coordinates   1198666..1199616 (+) Length   316 a.a.
NCBI ID   WP_011049891.1    Uniprot ID   -
Organism   Campylobacter jejuni strain RM1221     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1186964..1209722 1198666..1199616 within 0


Gene organization within MGE regions


Location: 1186964..1209722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I2453_RS06225 pglI 1186964..1187893 (-) 930 WP_002867212.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  I2453_RS06230 pglH 1187886..1188965 (-) 1080 WP_002859237.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  I2453_RS06235 pglK 1188962..1190656 (-) 1695 WP_002867213.1 ABC-type lipopolysaccharide transporter PglK -
  I2453_RS06240 galE 1190650..1191636 (-) 987 WP_002867214.1 UDP-glucose 4-epimerase GalE -
  I2453_RS06245 - 1191695..1192489 (-) 795 WP_002860221.1 3'-5' exonuclease -
  I2453_RS06250 waaC 1192556..1193584 (+) 1029 WP_011049885.1 lipopolysaccharide heptosyltransferase I -
  I2453_RS06255 htrB 1193574..1194461 (+) 888 WP_002867215.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  I2453_RS06260 - 1194458..1196008 (+) 1551 WP_002867216.1 glycosyltransferase -
  I2453_RS06265 - 1195977..1196864 (-) 888 WP_011049886.1 glycosyltransferase family 2 protein -
  I2453_RS06270 - 1196857..1197744 (-) 888 WP_011049889.1 glycosyltransferase -
  I2453_RS06275 waaV 1197798..1198607 (-) 810 WP_011049890.1 glycosyltransferase family 2 protein -
  I2453_RS06280 waaF 1198666..1199616 (+) 951 WP_011049891.1 lipopolysaccharide heptosyltransferase II Regulator
  I2453_RS06285 - 1199602..1200366 (-) 765 WP_002884483.1 glycosyltransferase family 25 protein -
  I2453_RS06290 gmhA 1200366..1200926 (-) 561 WP_002869619.1 D-sedoheptulose 7-phosphate isomerase -
  I2453_RS06295 rfaE1 1200923..1202308 (-) 1386 WP_002867399.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  I2453_RS06300 rfaD 1202301..1203254 (-) 954 WP_002867398.1 ADP-glyceromanno-heptose 6-epimerase -
  I2453_RS06305 gmhB 1203255..1203816 (-) 562 Protein_1256 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  I2453_RS06310 - 1203897..1204199 (+) 303 WP_002852762.1 cytochrome c -
  I2453_RS06315 ccoS 1204222..1204428 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  I2453_RS06320 - 1204425..1206782 (-) 2358 WP_079254352.1 heavy metal translocating P-type ATPase -
  I2453_RS06325 rho 1206891..1208189 (+) 1299 WP_002852852.1 transcription termination factor Rho -
  I2453_RS06330 dnaX 1208193..1209722 (+) 1530 WP_002867395.1 DNA polymerase III subunit gamma/tau -

Sequence

Protein


Download         Length: 316 a.a.        Molecular weight: 36213.76 Da        Isoelectric Point: 10.2878

>NTDB_id=518852 I2453_RS06280 WP_011049891.1 1198666..1199616(+) (waaF) [Campylobacter jejuni strain RM1221]
MKIFIHLPTWLGDAVMASPALYVIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQALSLRKELGKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLHIKAHLKDLKLPFKLKFQSPLVLKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISAVYKVKTVAIFGPTKFTQTSPWQNENATLVHLNLACMPCMQKTCPLKHHKCMKDLKPEVIVKAIQNLTNI

Nucleotide


Download         Length: 951 bp        

>NTDB_id=518852 I2453_RS06280 WP_011049891.1 1198666..1199616(+) (waaF) [Campylobacter jejuni strain RM1221]
ATGAAAATTTTTATACACCTTCCCACTTGGCTAGGCGATGCGGTGATGGCTTCACCTGCTTTATACGTTATAAAAGAACA
TTTTAAAAATGCCCAATTTATCCTTTATGGATCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCCCGCTACAAACAAGCCCTATCTTTACGCAAAGAACTTGGTAAGATCGATTTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTACATATTCTTAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTCACATAAAAGCTCACCTTAAAGACTTAA
AACTTCCCTTTAAGCTAAAATTTCAAAGCCCTCTTGTCTTAAAAAATGGCAAAAAAATTCTAGGACTCAATCCTGGTGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAGATTTT
AATCTTTGGCGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACTACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGCCCTATGCACATAAGTGCAGTTTATAAGGTAAAAACCGTGGCTATTTTTGGCCCTACAAAATTTACTCAAACCTCACC
TTGGCAAAATGAAAACGCAACATTAGTGCATTTAAATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATTTAAAGCCTGAAGTGATTGTAAAAGCGATTCAAAATTTAACTAATATCTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(96-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

92.652

99.051

0.918