Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   IOO20_RS06265 Genome accession   NZ_CP066241
Coordinates   1198674..1199624 (+) Length   316 a.a.
NCBI ID   WP_011049891.1    Uniprot ID   -
Organism   Campylobacter jejuni strain RM1221     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1186972..1209730 1198674..1199624 within 0


Gene organization within MGE regions


Location: 1186972..1209730
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IOO20_RS06210 pglI 1186972..1187901 (-) 930 WP_002867212.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  IOO20_RS06215 pglH 1187894..1188973 (-) 1080 WP_002859237.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  IOO20_RS06220 pglK 1188970..1190664 (-) 1695 WP_002867213.1 ABC-type lipopolysaccharide transporter PglK -
  IOO20_RS06225 galE 1190658..1191644 (-) 987 WP_002867214.1 UDP-glucose 4-epimerase GalE -
  IOO20_RS06230 - 1191703..1192497 (-) 795 WP_002860221.1 3'-5' exonuclease -
  IOO20_RS06235 waaC 1192564..1193592 (+) 1029 WP_011049885.1 lipopolysaccharide heptosyltransferase I -
  IOO20_RS06240 htrB 1193582..1194469 (+) 888 WP_002867215.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  IOO20_RS06245 - 1194466..1196016 (+) 1551 WP_002867216.1 glycosyltransferase -
  IOO20_RS06250 - 1195985..1196872 (-) 888 WP_011049886.1 glycosyltransferase family 2 protein -
  IOO20_RS06255 - 1196865..1197752 (-) 888 WP_011049889.1 glycosyltransferase -
  IOO20_RS06260 waaV 1197806..1198615 (-) 810 WP_011049890.1 glycosyltransferase family 2 protein -
  IOO20_RS06265 waaF 1198674..1199624 (+) 951 WP_011049891.1 lipopolysaccharide heptosyltransferase II Regulator
  IOO20_RS06270 - 1199610..1200374 (-) 765 WP_002884483.1 glycosyltransferase family 25 protein -
  IOO20_RS06275 gmhA 1200374..1200934 (-) 561 WP_002869619.1 D-sedoheptulose 7-phosphate isomerase -
  IOO20_RS06280 rfaE1 1200931..1202316 (-) 1386 WP_002867399.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  IOO20_RS06285 rfaD 1202309..1203262 (-) 954 WP_002867398.1 ADP-glyceromanno-heptose 6-epimerase -
  IOO20_RS06290 gmhB 1203263..1203824 (-) 562 Protein_1246 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  IOO20_RS06295 - 1203905..1204207 (+) 303 WP_002852762.1 cytochrome c -
  IOO20_RS06300 ccoS 1204230..1204436 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  IOO20_RS06305 - 1204433..1206790 (-) 2358 WP_079254352.1 heavy metal translocating P-type ATPase -
  IOO20_RS06310 rho 1206899..1208197 (+) 1299 WP_002852852.1 transcription termination factor Rho -
  IOO20_RS06315 dnaX 1208201..1209730 (+) 1530 WP_002867395.1 DNA polymerase III subunit gamma/tau -

Sequence

Protein


Download         Length: 316 a.a.        Molecular weight: 36213.76 Da        Isoelectric Point: 10.2878

>NTDB_id=518822 IOO20_RS06265 WP_011049891.1 1198674..1199624(+) (waaF) [Campylobacter jejuni strain RM1221]
MKIFIHLPTWLGDAVMASPALYVIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQALSLRKELGKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLHIKAHLKDLKLPFKLKFQSPLVLKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISAVYKVKTVAIFGPTKFTQTSPWQNENATLVHLNLACMPCMQKTCPLKHHKCMKDLKPEVIVKAIQNLTNI

Nucleotide


Download         Length: 951 bp        

>NTDB_id=518822 IOO20_RS06265 WP_011049891.1 1198674..1199624(+) (waaF) [Campylobacter jejuni strain RM1221]
ATGAAAATTTTTATACACCTTCCCACTTGGCTAGGCGATGCGGTGATGGCTTCACCTGCTTTATACGTTATAAAAGAACA
TTTTAAAAATGCCCAATTTATCCTTTATGGATCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCCCGCTACAAACAAGCCCTATCTTTACGCAAAGAACTTGGTAAGATCGATTTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTACATATTCTTAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTCACATAAAAGCTCACCTTAAAGACTTAA
AACTTCCCTTTAAGCTAAAATTTCAAAGCCCTCTTGTCTTAAAAAATGGCAAAAAAATTCTAGGACTCAATCCTGGTGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAGATTTT
AATCTTTGGCGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACTACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGCCCTATGCACATAAGTGCAGTTTATAAGGTAAAAACCGTGGCTATTTTTGGCCCTACAAAATTTACTCAAACCTCACC
TTGGCAAAATGAAAACGCAACATTAGTGCATTTAAATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATTTAAAGCCTGAAGTGATTGTAAAAGCGATTCAAAATTTAACTAATATCTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(96-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

92.652

99.051

0.918