Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   I5435_RS22440 Genome accession   NZ_CP066226
Coordinates   4548866..4550242 (-) Length   458 a.a.
NCBI ID   WP_337983003.1    Uniprot ID   -
Organism   Lysinibacillus sp. C5.1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4543866..4555242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5435_RS22420 gltX 4544515..4545975 (-) 1461 WP_025220849.1 glutamate--tRNA ligase -
  I5435_RS22425 ispF 4546100..4546576 (-) 477 WP_024364393.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  I5435_RS22430 ispD 4546771..4547466 (-) 696 WP_024364394.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  I5435_RS22435 - 4547479..4548564 (-) 1086 WP_024364395.1 PIN/TRAM domain-containing protein -
  I5435_RS22440 radA 4548866..4550242 (-) 1377 WP_337983003.1 DNA repair protein RadA Machinery gene
  I5435_RS22445 - 4550353..4552797 (-) 2445 WP_024364397.1 ATP-dependent Clp protease ATP-binding subunit -
  I5435_RS22450 - 4552787..4553896 (-) 1110 WP_024364398.1 protein arginine kinase -
  I5435_RS22455 - 4553893..4554435 (-) 543 WP_024364399.1 UvrB/UvrC motif-containing protein -
  I5435_RS22460 - 4554449..4554919 (-) 471 WP_024364400.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50062.85 Da        Isoelectric Point: 7.3824

>NTDB_id=518615 I5435_RS22440 WP_337983003.1 4548866..4550242(-) (radA) [Lysinibacillus sp. C5.1]
MAKKKTKFVCTGCGYESAKWMGRCPGCGEWNKMVEEVEVVAKGPRGAFQHSATVTQKAVPIIQVEAAEESRVATEMSELN
RVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSALLSNKGHRVLYISGEESIRQTKLRAERLGVVSQELYIYSETNLELLNQ
TIDDVQPKFVIVDSIQTVFHPEVTSAPGSVSQVRECTAELMRIAKTKGIAIFLVGHVTKEGQIAGPRILEHMVDTVLYFE
GERHHNHRILRSQKNRFGSTNEIAIFEMLQGGLKEVLNPSELFLQERSQGAAGSTIVASMEGTRPILVEIQSLVTPTSFN
YPKRMATGVDQNRVQLLMAVLEKRMGLMLQAQDAYIKVAGGVKLDEPAIDLAVLTSIVSSFKDQAVRGTDCFIGEVGLTG
EVRRVTRIEQRVLEAAKLGFKRAFIPASNIGGWDFPQGIEIVGVETIKDALNACFREL

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=518615 I5435_RS22440 WP_337983003.1 4548866..4550242(-) (radA) [Lysinibacillus sp. C5.1]
ATGGCGAAGAAAAAAACTAAATTTGTCTGTACAGGTTGTGGCTATGAATCGGCAAAGTGGATGGGACGTTGTCCAGGATG
TGGTGAATGGAATAAAATGGTTGAGGAAGTGGAGGTTGTGGCGAAAGGCCCTAGAGGAGCATTCCAACACTCTGCAACCG
TCACGCAAAAAGCTGTACCTATTATCCAAGTAGAAGCGGCAGAGGAGTCTCGTGTAGCTACAGAAATGAGTGAACTCAAT
CGTGTCTTGGGCGGAGGAATTGTTCCGGGATCTCTAGTGTTAATCGGGGGTGATCCGGGTATCGGAAAATCGACATTGCT
ATTACAGGTGTCTGCGTTATTGTCCAATAAAGGTCATCGTGTGCTCTATATTTCTGGTGAGGAATCCATTCGACAAACGA
AACTGCGTGCAGAGCGTTTAGGGGTTGTATCTCAAGAGCTGTATATATATTCGGAGACGAATTTAGAATTGTTAAATCAA
ACAATCGACGATGTACAACCAAAGTTTGTTATTGTGGATTCTATCCAAACGGTGTTCCATCCAGAGGTGACAAGTGCGCC
AGGTAGTGTATCGCAAGTACGTGAATGTACAGCGGAGCTAATGCGGATAGCGAAAACAAAAGGCATTGCCATTTTCTTAG
TTGGACATGTGACGAAGGAAGGGCAAATTGCGGGACCGCGTATTTTAGAGCATATGGTAGATACAGTGCTGTATTTCGAA
GGAGAACGACATCACAACCACCGTATTTTACGGAGCCAAAAAAACCGCTTTGGCTCTACAAATGAAATAGCTATCTTTGA
AATGCTTCAGGGGGGCTTAAAGGAAGTTTTGAATCCGTCTGAGCTATTTTTACAGGAACGTTCACAAGGTGCAGCAGGTT
CAACAATCGTGGCATCGATGGAGGGAACAAGGCCAATTTTAGTAGAAATTCAATCACTTGTAACCCCTACAAGCTTTAAT
TATCCGAAACGGATGGCAACAGGGGTGGACCAAAACCGTGTGCAATTGCTTATGGCAGTGCTTGAAAAGCGCATGGGCCT
CATGTTACAGGCACAAGATGCATATATTAAAGTTGCTGGCGGAGTAAAGCTAGATGAACCTGCCATTGACTTAGCTGTCT
TGACGAGTATTGTGTCTAGCTTTAAGGATCAGGCAGTACGGGGAACAGATTGCTTTATTGGAGAAGTAGGGTTAACAGGA
GAGGTTCGTCGTGTCACACGAATTGAACAACGTGTGCTAGAGGCGGCAAAGCTAGGGTTTAAGCGAGCATTTATCCCTGC
TTCTAATATTGGCGGTTGGGATTTCCCACAAGGTATTGAAATAGTTGGTGTGGAAACAATTAAAGATGCATTAAATGCCT
GCTTTAGAGAGCTTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(65-221)

Subunit ChlI of Mg-chelatase (ChlI)

(346-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

70.044

99.127

0.694
  radA Streptococcus pneumoniae Rx1

62.281

99.563

0.62
  radA Streptococcus pneumoniae D39

62.281

99.563

0.62
  radA Streptococcus pneumoniae R6

62.281

99.563

0.62
  radA Streptococcus pneumoniae TIGR4

62.281

99.563

0.62
  radA Streptococcus mitis NCTC 12261

62.281

99.563

0.62
  radA Streptococcus mitis SK321

62.281

99.563

0.62