Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   JD965_RS01740 Genome accession   NZ_CP066219
Coordinates   322077..322514 (-) Length   145 a.a.
NCBI ID   WP_047476187.1    Uniprot ID   -
Organism   Bacillus siamensis strain B28     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 317077..327514
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JD965_RS20190 - 317779..317908 (-) 130 Protein_300 hypothetical protein -
  JD965_RS01710 (JD965_01710) - 318056..319258 (+) 1203 WP_047476182.1 GTP-binding protein -
  JD965_RS01715 (JD965_01715) - 319322..320458 (+) 1137 WP_029575240.1 zinc-dependent alcohol dehydrogenase -
  JD965_RS01720 (JD965_01720) - 320475..320909 (+) 435 WP_045926273.1 RDD family protein -
  JD965_RS01725 (JD965_01725) - 321027..321305 (+) 279 WP_411810548.1 YckD family protein -
  JD965_RS01730 (JD965_01730) - 321360..321503 (+) 144 Protein_305 family 1 glycosylhydrolase -
  JD965_RS01735 (JD965_01735) nin/comJ 321657..322055 (-) 399 WP_047476185.1 competence protein ComJ Regulator
  JD965_RS01740 (JD965_01740) nucA/comI 322077..322514 (-) 438 WP_047476187.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  JD965_RS01745 (JD965_01745) hxlB 322871..323428 (-) 558 WP_064777947.1 6-phospho-3-hexuloisomerase -
  JD965_RS01750 (JD965_01750) hxlA 323425..324060 (-) 636 WP_016936796.1 3-hexulose-6-phosphate synthase -
  JD965_RS01755 (JD965_01755) - 324293..324655 (+) 363 WP_016936795.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16082.39 Da        Isoelectric Point: 8.4850

>NTDB_id=518476 JD965_RS01740 WP_047476187.1 322077..322514(-) (nucA/comI) [Bacillus siamensis strain B28]
MKTLKALLLVLLIIAGAVAALIKGDIFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHPSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=518476 JD965_RS01740 WP_047476187.1 322077..322514(-) (nucA/comI) [Bacillus siamensis strain B28]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTCTCATCATTGCCGGAGCAGTTGCGGCCCTGATCAAGGGTGATATTTT
CTCAGATAACGAGCAGACCGTTCAGAAAAGCGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATCCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTAAAAACGGGGTACGATAGAGATGAATGGCCCATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGGGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCAGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.016

86.897

0.634