Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   JD965_RS00590 Genome accession   NZ_CP066219
Coordinates   106978..108357 (+) Length   459 a.a.
NCBI ID   WP_016935915.1    Uniprot ID   A0AAE2NZ63
Organism   Bacillus siamensis strain B28     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 101978..113357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JD965_RS00570 (JD965_00570) ctsR 102326..102790 (+) 465 WP_016935919.1 transcriptional regulator CtsR -
  JD965_RS00575 (JD965_00575) - 102804..103361 (+) 558 WP_016935918.1 UvrB/UvrC motif-containing protein -
  JD965_RS00580 (JD965_00580) - 103361..104452 (+) 1092 WP_016935917.1 protein arginine kinase -
  JD965_RS00585 (JD965_00585) clpC 104449..106884 (+) 2436 WP_047477921.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  JD965_RS00590 (JD965_00590) radA 106978..108357 (+) 1380 WP_016935915.1 DNA repair protein RadA Machinery gene
  JD965_RS00595 (JD965_00595) disA 108361..109443 (+) 1083 WP_003156401.1 DNA integrity scanning diadenylate cyclase DisA -
  JD965_RS00600 (JD965_00600) - 109557..110657 (+) 1101 WP_045927008.1 PIN/TRAM domain-containing protein -
  JD965_RS00605 (JD965_00605) ispD 110671..111369 (+) 699 WP_198698926.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  JD965_RS00610 (JD965_00610) ispF 111362..111838 (+) 477 WP_016935912.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49448.87 Da        Isoelectric Point: 7.4033

>NTDB_id=518473 JD965_RS00590 WP_016935915.1 106978..108357(+) (radA) [Bacillus siamensis strain B28]
MAKSKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVQTKLEEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLADTAGSVLYISGEESVKQTKLRADRLGINSQLLHVLSETDMEYIS
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGASGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMVIPEANAGGWTIPKGIEVVGVANVAEALRTSLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=518473 JD965_RS00590 WP_016935915.1 106978..108357(+) (radA) [Bacillus siamensis strain B28]
ATGGCTAAATCAAAAACAAAATTCATCTGCCACTCATGCGGTTATGAATCCGCAAAATGGATGGGGAAATGCCCGGGCTG
CGGGGCTTGGAATACAATGGTGGAAGAAACCATCAAAAAAGCGCCGGCAAATCGGCGGGCGGCTTTCTCCCACTCCGTTC
AAACCGTCCAGAAACCATCACCCATTACATCAATTGAAACATCAGAAGAACCCCGTGTTCAAACCAAGCTCGAAGAATTC
AACAGAGTGCTTGGCGGCGGCGTTGTAAAGGGCTCGTTAGTATTAATCGGCGGCGACCCCGGCATCGGGAAGTCAACGCT
CCTTCTTCAAGTATCCGCACAATTAGCAGACACGGCCGGCAGCGTGCTTTACATTTCCGGGGAGGAATCCGTGAAGCAAA
CGAAGCTGCGCGCTGACAGGCTTGGAATCAACAGCCAATTGTTACATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAAACCCGCATTTGTTGTCGTAGACTCCATTCAGACCGTTTACCAAAGCGACATTACCTCAGC
TCCCGGCAGTGTATCACAAGTAAGAGAGTGTACGGCTGAATTGATGAAAATCGCGAAAACAAACGGCATTCCGATTTTTA
TCGTCGGACACGTCACAAAAGAAGGATCGATCGCAGGGCCGAGATTGCTTGAGCATATGGTCGATACCGTTCTATACTTT
GAAGGAGAGCGGCACCATACATTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGCTCAACAAATGAAATGGGCATCTT
TGAAATGCGGGAAGAGGGGCTTACAGAAGTGCTGAACCCTTCGGAAATTTTCTTGGAAGAGCGTTCTGCCGGGGCTTCGG
GTTCGAGTATCGTTGCGTCAATGGAAGGAACGAGACCGATCCTGGTTGAAATTCAGGCGCTCATATCACCGACCAGCTTT
GGAAACCCGCGCCGCATGGCTACAGGGATTGACCACAATAGGGTGTCGTTAATCATGGCTGTGCTTGAGAAGCGGGTGGG
GCTTTTGCTTCAGAATCAAGATGCATATTTAAAAGTCGCCGGCGGGGTCAAACTGGATGAACCGGCGATTGATTTGGCAG
TAGCCGTCAGCATTGCTTCAAGCTTCAGAGATACACCGCCGAATCCTGCAGATTGCTTTATCGGTGAAGTGGGTCTGACA
GGAGAAGTCCGCAGGGTGTCAAGAATCGAACAGCGCGTAAAAGAAGCGGCGAAACTCGGCTTCAAGCGTATGGTCATACC
TGAGGCGAATGCAGGCGGATGGACAATACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(71-222)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

93.013

99.782

0.928
  radA Streptococcus pneumoniae Rx1

63.797

98.693

0.63
  radA Streptococcus pneumoniae D39

63.797

98.693

0.63
  radA Streptococcus pneumoniae R6

63.797

98.693

0.63
  radA Streptococcus pneumoniae TIGR4

63.797

98.693

0.63
  radA Streptococcus mitis NCTC 12261

63.797

98.693

0.63
  radA Streptococcus mitis SK321

63.576

98.693

0.627