Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   JC775_RS14295 Genome accession   NZ_CP066192
Coordinates   2861231..2861665 (-) Length   144 a.a.
NCBI ID   WP_012095165.1    Uniprot ID   A7GS32
Organism   Bacillus cytotoxicus strain E17.4     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2856231..2866665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JC775_RS14280 (JC775_14280) - 2856328..2857863 (+) 1536 WP_087099055.1 MDR family MFS transporter -
  JC775_RS14285 (JC775_14285) metE 2858453..2860741 (+) 2289 WP_048721820.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  JC775_RS14290 (JC775_14290) comJ 2860820..2861209 (-) 390 WP_012095164.1 competence protein ComJ -
  JC775_RS14295 (JC775_14295) nucA/comI 2861231..2861665 (-) 435 WP_012095165.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  JC775_RS14300 (JC775_14300) - 2861906..2862652 (+) 747 WP_012095166.1 DUF3967 domain-containing protein -
  JC775_RS14305 (JC775_14305) - 2862726..2864000 (-) 1275 WP_012095167.1 HAMP domain-containing sensor histidine kinase -
  JC775_RS14310 (JC775_14310) - 2864309..2864509 (+) 201 WP_048721818.1 hypothetical protein -
  JC775_RS14315 (JC775_14315) - 2864608..2865771 (+) 1164 WP_048721816.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16090.47 Da        Isoelectric Point: 9.4939

>NTDB_id=518353 JC775_RS14295 WP_012095165.1 2861231..2861665(-) (nucA/comI) [Bacillus cytotoxicus strain E17.4]
MKQLKGIIISIIAVLSILVAVYEALIPTEQAKQKDGTYDQVLEFPKDRYPETGKHIENAIKEGHSKICTIDRNGAGDRRK
QSLAPYPPKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYQDGTRVKFVVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=518353 JC775_RS14295 WP_012095165.1 2861231..2861665(-) (nucA/comI) [Bacillus cytotoxicus strain E17.4]
ATGAAACAATTGAAAGGGATTATTATTTCAATCATTGCGGTTCTTTCTATTTTAGTGGCTGTTTATGAAGCGCTTATACC
GACAGAACAAGCAAAACAAAAGGACGGTACATATGATCAAGTTCTAGAATTTCCGAAAGATCGTTATCCAGAAACCGGAA
AGCATATAGAAAATGCAATAAAGGAAGGACATTCTAAAATCTGTACGATTGATAGAAATGGAGCTGGTGACAGACGAAAG
CAATCTTTAGCTCCATATCCACCAAAAAAAGGATACGATCGTGATGAATGGCCGATGGCAATGTGTAAGGAAGGAGGCAA
GGGGGCTCATATTGAATATATTAGTCCGGCTGATAATAGAGGAGCTGGCTCATGGGTCGGTAATAAATTGGATAAATACC
AAGATGGTACACGTGTGAAATTTGTAGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7GS32

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

63.333

83.333

0.528