Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   JC775_RS00810 Genome accession   NZ_CP066192
Coordinates   164055..165431 (+) Length   458 a.a.
NCBI ID   WP_011983221.1    Uniprot ID   A7GJZ4
Organism   Bacillus cytotoxicus strain E17.4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 159055..170431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JC775_RS00790 (JC775_00790) - 159276..159737 (+) 462 WP_011983217.1 CtsR family transcriptional regulator -
  JC775_RS00795 (JC775_00795) - 159893..160441 (+) 549 WP_011983218.1 UvrB/UvrC motif-containing protein -
  JC775_RS00800 (JC775_00800) - 160446..161516 (+) 1071 WP_011983219.1 protein arginine kinase -
  JC775_RS00805 (JC775_00805) clpC 161532..163967 (+) 2436 WP_087094328.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  JC775_RS00810 (JC775_00810) radA 164055..165431 (+) 1377 WP_011983221.1 DNA repair protein RadA Machinery gene
  JC775_RS00815 (JC775_00815) disA 165435..166508 (+) 1074 WP_011983222.1 DNA integrity scanning diadenylate cyclase DisA -
  JC775_RS00820 (JC775_00820) - 166670..167782 (+) 1113 WP_011983223.1 PIN/TRAM domain-containing protein -
  JC775_RS00825 (JC775_00825) ispD 167799..168479 (+) 681 WP_011983224.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  JC775_RS00830 (JC775_00830) ispF 168589..169065 (+) 477 WP_011983225.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50033.72 Da        Isoelectric Point: 7.3900

>NTDB_id=518329 JC775_RS00810 WP_011983221.1 164055..165431(+) (radA) [Bacillus cytotoxicus strain E17.4]
MAKKKTKFICQECGYQSPKYMGRCPGCGQWNTLVEEIEQVVSSRRLNYANAIQTEVTKPRRLIEVETKSEARIETQFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLANSAYDVLYISGEESAKQIKLRADRLHVNGRNLFVVSETDLQRIA
THIEEMNPSFVVIDSIQTIYLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRAGLLLQNQDAYLKVAGGLKLDEPAIDLAVAISIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPEGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=518329 JC775_RS00810 WP_011983221.1 164055..165431(+) (radA) [Bacillus cytotoxicus strain E17.4]
ATGGCTAAGAAGAAAACGAAATTCATATGTCAAGAATGCGGTTATCAATCACCGAAATATATGGGAAGATGCCCTGGTTG
TGGACAATGGAATACACTTGTTGAAGAGATAGAACAAGTTGTATCATCTAGACGCCTTAATTATGCAAATGCGATTCAAA
CAGAGGTAACAAAACCAAGAAGGCTTATAGAAGTAGAAACAAAATCAGAAGCGCGTATTGAGACACAATTTCAAGAATTT
AATCGTGTATTAGGTGGAGGTATTGTTGATGGTTCCCTTGTTCTTATCGGAGGAGATCCTGGAATTGGGAAATCGACTTT
ACTGTTGCAAATATCATCACAATTAGCAAATTCTGCTTATGATGTTCTGTATATATCAGGAGAAGAATCCGCAAAACAAA
TCAAGCTTCGTGCAGATCGTTTACATGTAAATGGCCGGAATCTGTTCGTTGTTTCAGAAACAGATTTACAGCGCATTGCA
ACTCATATTGAAGAAATGAATCCCTCTTTTGTTGTAATTGATTCTATTCAAACCATTTATCTACCGGAAGTTACTTCAGC
ACCTGGAAGTGTGGCACAAGTACGTGAATGTACGGCGGAACTGATGAAACTTGCGAAAACGAAAGGAATCCCCATTTTTA
TTGTTGGGCATGTGACAAAAGAAGGTGCGATTGCAGGGCCACGTATGCTAGAACATATGGTAGATGCGGTTCTTTATTTT
GAAGGAGATCGTCATCATACATATCGTATTTTGCGAGCAGTAAAAAACCGATTTGGTTCTACAAATGAAATGGGTATTTT
TGAAATGAAAGAGCTGGGTCTTGCAGAAGTATTGAATCCTTCTGAAATTTTCCTAGAAGAAAGACCGGTCGGAGTTGCAG
GGTCTACAGTTGTTGCCTCAATGGAAGGAACAAGACCTGTTTTAGTAGAAATACAAGCATTAATCTCTCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCCACAGGAATTGATCATAATCGTGTATCTCTTATTATGGCAGTGTTGGAAAAGAGAGCCGG
ATTATTGTTGCAAAATCAAGATGCGTATTTGAAAGTGGCTGGAGGTTTGAAGTTAGATGAACCAGCGATTGACTTAGCGG
TTGCAATAAGCATTGCTTCAAGTTTTCGAGATAAATCTACGGCACCGACTGATGCGGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCTGCTAAATTAGGATTTCAACGTGCAATTATCCC
GCGGAAAAATTTAGGTGGATGGACAATTCCTGAGGGGATTGAAGTGGTTGGTGTTTCCAATTTAGGCGAGGCGCTGCGAT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(61-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7GJZ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

75.599

100

0.758
  radA Streptococcus mitis NCTC 12261

64.018

98.908

0.633
  radA Streptococcus pneumoniae Rx1

63.797

98.908

0.631
  radA Streptococcus pneumoniae D39

63.797

98.908

0.631
  radA Streptococcus pneumoniae R6

63.797

98.908

0.631
  radA Streptococcus pneumoniae TIGR4

63.797

98.908

0.631
  radA Streptococcus mitis SK321

63.797

98.908

0.631