Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   JC772_RS02470 Genome accession   NZ_CP066187
Coordinates   449724..450767 (-) Length   347 a.a.
NCBI ID   WP_087095343.1    Uniprot ID   -
Organism   Bacillus cytotoxicus strain E28.3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 444724..455767
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JC772_RS02430 (JC772_02430) - 444916..445545 (+) 630 WP_012095379.1 VC0807 family protein -
  JC772_RS02435 (JC772_02435) - 445691..445891 (-) 201 WP_048723620.1 YqzE family protein -
  JC772_RS02440 (JC772_02440) - 445930..446427 (-) 498 WP_012095381.1 shikimate kinase -
  JC772_RS02445 (JC772_02445) comGG 446827..447201 (-) 375 WP_048723614.1 competence type IV pilus minor pilin ComGG -
  JC772_RS02450 (JC772_02450) comGF 447198..447668 (-) 471 WP_041810544.1 competence type IV pilus minor pilin ComGF -
  JC772_RS22060 - 447647..447964 (-) 318 WP_235436438.1 type II secretion system protein -
  JC772_RS02455 (JC772_02455) comGD 447942..448388 (-) 447 Protein_497 competence type IV pilus minor pilin ComGD -
  JC772_RS02460 (JC772_02460) comGC 448385..448684 (-) 300 WP_012095385.1 competence type IV pilus major pilin ComGC -
  JC772_RS02465 (JC772_02465) comGB 448697..449737 (-) 1041 WP_041810011.1 competence type IV pilus assembly protein ComGB -
  JC772_RS02470 (JC772_02470) comGA 449724..450767 (-) 1044 WP_087095343.1 competence type IV pilus ATPase ComGA Machinery gene
  JC772_RS02475 (JC772_02475) - 450972..451667 (+) 696 WP_012095431.1 helix-turn-helix domain-containing protein -
  JC772_RS02480 (JC772_02480) - 451809..452051 (+) 243 WP_012095433.1 DUF2626 domain-containing protein -
  JC772_RS02485 (JC772_02485) - 452195..453550 (+) 1356 WP_269147661.1 L-cystine transporter -
  JC772_RS02490 (JC772_02490) - 453682..453870 (-) 189 Protein_504 DUF3912 family protein -
  JC772_RS02495 (JC772_02495) - 454128..454439 (+) 312 WP_012095437.1 hypothetical protein -
  JC772_RS02500 (JC772_02500) - 454480..454971 (-) 492 WP_012095438.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39151.57 Da        Isoelectric Point: 9.3683

>NTDB_id=518280 JC772_RS02470 WP_087095343.1 449724..450767(-) (comGA) [Bacillus cytotoxicus strain E28.3]
MNDIEHFANKIMKEACAVQASDLHIVPNRENVMIQLRIGQDLMTKHYIEKQFGERLISHFKFLASMDIGERRKPQNGSLY
LQIDGQEVHLRLSTLPTVYLESLVIRLHLQASIQPLSHLSLFPSSAKKLLSFLHHSHGLLIFTGPTGSGKTTTMYALLEV
AKKGQTRRIVTLEDPVEKRKNGLLQIQINEKAGITYETGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAVLRLMDFGISRQEIEQSLLAVAAQRLVELKCPFCKGKCSPLCKSIRQVRQASIYELLYGHELKKAIREANGER
VVYRYETLQSSLKKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=518280 JC772_RS02470 WP_087095343.1 449724..450767(-) (comGA) [Bacillus cytotoxicus strain E28.3]
ATGAATGATATTGAACATTTTGCCAATAAGATTATGAAAGAAGCTTGTGCAGTACAGGCATCTGATTTACACATTGTACC
CAATCGGGAGAATGTAATGATTCAATTGCGCATTGGACAGGACTTAATGACGAAACATTATATTGAAAAGCAGTTTGGAG
AGAGACTCATTTCACATTTCAAATTTTTAGCTTCTATGGATATTGGAGAAAGGCGTAAACCACAAAATGGCTCACTTTAT
TTACAAATTGATGGTCAAGAAGTACATTTACGATTATCCACACTTCCTACAGTATATTTGGAAAGCCTCGTTATTCGTTT
GCATTTGCAAGCGTCTATCCAACCATTATCACATCTCTCTTTATTCCCGAGTTCAGCGAAGAAACTGCTCTCTTTTTTAC
ATCACTCACATGGATTGCTTATATTTACAGGACCAACTGGATCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTT
GCCAAAAAAGGTCAAACACGCCGGATTGTTACATTGGAAGATCCGGTGGAGAAAAGAAAGAACGGTTTATTACAAATTCA
AATTAATGAAAAAGCAGGGATTACGTATGAGACAGGTTTAAAGGCAATTTTACGGCATGACCCAGATATTATTTTAGTGG
GGGAAATTCGTGATGAAGAAACAGCAAAAGTGGCTGTACGTGCAAGTTTAACAGGTCATTTAGTAATGACAACGCTGCAT
ACAAATGATGCGAAAGGTGCTGTATTGCGCTTGATGGATTTTGGAATTAGCAGACAGGAAATTGAACAGTCGTTATTAGC
TGTAGCCGCTCAGCGCCTTGTAGAGTTGAAATGTCCGTTTTGTAAAGGGAAGTGTTCACCATTATGTAAATCGATACGCC
AAGTACGGCAAGCAAGCATTTATGAGTTGCTATATGGTCATGAATTGAAGAAAGCCATTCGCGAAGCGAATGGCGAACGT
GTGGTGTATCGCTATGAAACATTACAATCTTCATTAAAGAAAGGCTATGCTTTAGGATTTTTAGAAGAGGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.637

100

0.576
  pilB Glaesserella parasuis strain SC1401

43.945

83.285

0.366
  pilB Vibrio campbellii strain DS40M4

35.411

100

0.36