Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   JC777_RS05010 Genome accession   NZ_CP066184
Coordinates   822951..824327 (-) Length   458 a.a.
NCBI ID   WP_087097527.1    Uniprot ID   -
Organism   Bacillus cytotoxicus strain SM1.1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 817951..829327
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JC777_RS04990 (JC777_04990) ispF 819317..819793 (-) 477 WP_011983225.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  JC777_RS04995 (JC777_04995) ispD 819903..820583 (-) 681 WP_011983224.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  JC777_RS05000 (JC777_05000) - 820600..821712 (-) 1113 WP_087097529.1 PIN/TRAM domain-containing protein -
  JC777_RS05005 (JC777_05005) disA 821874..822947 (-) 1074 WP_011983222.1 DNA integrity scanning diadenylate cyclase DisA -
  JC777_RS05010 (JC777_05010) radA 822951..824327 (-) 1377 WP_087097527.1 DNA repair protein RadA Machinery gene
  JC777_RS05015 (JC777_05015) clpC 824415..826850 (-) 2436 WP_011983220.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  JC777_RS05020 (JC777_05020) - 826866..827936 (-) 1071 WP_087097525.1 protein arginine kinase -
  JC777_RS05025 (JC777_05025) - 827941..828489 (-) 549 WP_011983218.1 UvrB/UvrC motif-containing protein -
  JC777_RS05030 (JC777_05030) - 828645..829106 (-) 462 WP_011983217.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50003.70 Da        Isoelectric Point: 7.3900

>NTDB_id=518180 JC777_RS05010 WP_087097527.1 822951..824327(-) (radA) [Bacillus cytotoxicus strain SM1.1]
MAKKKTKFICQECGYQSPKYMGRCPGCGQWNTLVEEIEQVVSSRRLNYANAIQTEVTKPRRLIEVETKSEARIETQFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLANSAYDVLYISGEESAKQIKLRADRLHVNGRNLFVVSETDLQRIA
AHIEEMNPSFVVIDSIQTIYLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRAGLLLQNQDAYLKVAGGLKLDEPAIDLAVAISIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPEGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=518180 JC777_RS05010 WP_087097527.1 822951..824327(-) (radA) [Bacillus cytotoxicus strain SM1.1]
ATGGCTAAGAAGAAAACGAAATTCATATGTCAAGAATGCGGTTATCAATCACCGAAATATATGGGAAGATGCCCTGGTTG
TGGACAATGGAATACACTTGTTGAAGAGATAGAACAAGTTGTATCATCTAGACGCCTTAATTATGCAAATGCGATTCAAA
CAGAGGTAACAAAACCAAGAAGGCTTATAGAAGTAGAAACAAAATCAGAAGCGCGTATTGAGACACAATTTCAAGAATTT
AATCGTGTATTAGGTGGAGGTATTGTTGATGGTTCCCTTGTTCTTATCGGAGGAGATCCTGGAATTGGGAAATCGACTTT
ACTGTTGCAAATATCATCACAATTAGCAAATTCTGCTTATGATGTTCTGTATATATCAGGAGAAGAATCCGCAAAACAAA
TCAAGCTTCGTGCAGATCGTTTACATGTAAATGGCCGGAATCTGTTCGTTGTTTCAGAAACAGATTTACAGCGCATTGCA
GCTCATATTGAAGAAATGAATCCCTCTTTTGTTGTAATTGATTCTATTCAAACCATTTATCTACCGGAAGTTACTTCAGC
ACCTGGAAGTGTGGCACAAGTACGTGAATGTACGGCGGAACTGATGAAACTTGCGAAAACGAAAGGAATCCCCATTTTTA
TTGTTGGGCATGTGACAAAAGAAGGTGCGATTGCAGGGCCACGTATGCTAGAACATATGGTAGATGCGGTTCTTTATTTT
GAAGGAGATCGTCATCATACATATCGTATTTTGCGAGCAGTAAAAAACCGATTTGGTTCTACAAATGAAATGGGTATTTT
TGAAATGAAAGAGCTGGGTCTTGCAGAAGTATTGAATCCTTCTGAAATTTTCCTAGAAGAAAGACCGGTCGGAGTTGCAG
GGTCTACAGTTGTTGCCTCAATGGAAGGAACAAGACCTGTTTTAGTAGAAATACAAGCATTAATCTCTCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCCACAGGAATTGATCATAATCGTGTATCTCTTATTATGGCAGTGTTGGAAAAGAGAGCCGG
ATTATTGTTGCAAAATCAAGATGCGTATTTGAAAGTGGCTGGAGGTTTGAAGTTAGATGAACCAGCGATTGACTTAGCGG
TTGCAATAAGCATTGCTTCAAGTTTTCGAGATAAATCTACGGCACCGACTGATGCGGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCTGCTAAATTAGGATTTCAACGTGCAATTATCCC
GCGGAAAAATTTAGGTGGATGGACAATTCCTGAGGGGATTGAAGTGGTTGGTGTTTCCAATTTAGGCGAGGCGCTGCGAT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(61-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

75.599

100

0.758
  radA Streptococcus pneumoniae Rx1

64.018

98.908

0.633
  radA Streptococcus pneumoniae D39

64.018

98.908

0.633
  radA Streptococcus pneumoniae R6

64.018

98.908

0.633
  radA Streptococcus pneumoniae TIGR4

64.018

98.908

0.633
  radA Streptococcus mitis SK321

64.018

98.908

0.633
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631