Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   JC774_RS10595 Genome accession   NZ_CP066179
Coordinates   2006809..2007243 (+) Length   144 a.a.
NCBI ID   WP_012095165.1    Uniprot ID   A7GS32
Organism   Bacillus cytotoxicus strain SM2.8     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2001809..2012243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JC774_RS10575 (JC774_10575) - 2002703..2003866 (-) 1164 WP_048721816.1 aminotransferase A -
  JC774_RS10580 (JC774_10580) - 2003965..2004165 (-) 201 WP_048721818.1 hypothetical protein -
  JC774_RS10585 (JC774_10585) - 2004474..2005748 (+) 1275 WP_012095167.1 HAMP domain-containing sensor histidine kinase -
  JC774_RS10590 (JC774_10590) - 2005822..2006568 (-) 747 WP_012095166.1 DUF3967 domain-containing protein -
  JC774_RS10595 (JC774_10595) nucA/comI 2006809..2007243 (+) 435 WP_012095165.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  JC774_RS10600 (JC774_10600) comJ 2007265..2007654 (+) 390 WP_012095164.1 competence protein ComJ -
  JC774_RS10605 (JC774_10605) metE 2007733..2010021 (-) 2289 WP_048721820.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  JC774_RS10610 (JC774_10610) - 2010611..2012146 (-) 1536 WP_087099055.1 MDR family MFS transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16090.47 Da        Isoelectric Point: 9.4939

>NTDB_id=518154 JC774_RS10595 WP_012095165.1 2006809..2007243(+) (nucA/comI) [Bacillus cytotoxicus strain SM2.8]
MKQLKGIIISIIAVLSILVAVYEALIPTEQAKQKDGTYDQVLEFPKDRYPETGKHIENAIKEGHSKICTIDRNGAGDRRK
QSLAPYPPKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYQDGTRVKFVVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=518154 JC774_RS10595 WP_012095165.1 2006809..2007243(+) (nucA/comI) [Bacillus cytotoxicus strain SM2.8]
ATGAAACAATTGAAAGGGATTATTATTTCAATCATTGCGGTTCTTTCTATTTTAGTGGCTGTTTATGAAGCGCTTATACC
GACAGAACAAGCAAAACAAAAGGACGGTACATATGATCAAGTTCTAGAATTTCCGAAAGATCGTTATCCAGAAACCGGAA
AGCATATAGAAAATGCAATAAAGGAAGGACATTCTAAAATCTGTACGATTGATAGAAATGGAGCTGGTGACAGACGAAAG
CAATCTTTAGCTCCATATCCACCAAAAAAAGGATACGATCGTGATGAATGGCCGATGGCAATGTGTAAGGAAGGAGGCAA
GGGGGCTCATATTGAATATATTAGTCCGGCTGATAATAGAGGAGCTGGCTCATGGGTCGGTAATAAATTGGATAAATACC
AAGATGGTACACGTGTGAAATTTGTAGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7GS32

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

63.333

83.333

0.528