Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   JC774_RS03200 Genome accession   NZ_CP066179
Coordinates   596400..597776 (-) Length   458 a.a.
NCBI ID   WP_087097527.1    Uniprot ID   -
Organism   Bacillus cytotoxicus strain SM2.8     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 591400..602776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JC774_RS03180 (JC774_03180) ispF 592766..593242 (-) 477 WP_011983225.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  JC774_RS03185 (JC774_03185) ispD 593352..594032 (-) 681 WP_011983224.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  JC774_RS03190 (JC774_03190) - 594049..595161 (-) 1113 WP_087097529.1 PIN/TRAM domain-containing protein -
  JC774_RS03195 (JC774_03195) disA 595323..596396 (-) 1074 WP_011983222.1 DNA integrity scanning diadenylate cyclase DisA -
  JC774_RS03200 (JC774_03200) radA 596400..597776 (-) 1377 WP_087097527.1 DNA repair protein RadA Machinery gene
  JC774_RS03205 (JC774_03205) clpC 597864..600299 (-) 2436 WP_011983220.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  JC774_RS03210 (JC774_03210) - 600315..601385 (-) 1071 WP_087097525.1 protein arginine kinase -
  JC774_RS03215 (JC774_03215) - 601390..601938 (-) 549 WP_011983218.1 UvrB/UvrC motif-containing protein -
  JC774_RS03220 (JC774_03220) - 602094..602555 (-) 462 WP_011983217.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50003.70 Da        Isoelectric Point: 7.3900

>NTDB_id=518130 JC774_RS03200 WP_087097527.1 596400..597776(-) (radA) [Bacillus cytotoxicus strain SM2.8]
MAKKKTKFICQECGYQSPKYMGRCPGCGQWNTLVEEIEQVVSSRRLNYANAIQTEVTKPRRLIEVETKSEARIETQFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLANSAYDVLYISGEESAKQIKLRADRLHVNGRNLFVVSETDLQRIA
AHIEEMNPSFVVIDSIQTIYLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRAGLLLQNQDAYLKVAGGLKLDEPAIDLAVAISIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPEGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=518130 JC774_RS03200 WP_087097527.1 596400..597776(-) (radA) [Bacillus cytotoxicus strain SM2.8]
ATGGCTAAGAAGAAAACGAAATTCATATGTCAAGAATGCGGTTATCAATCACCGAAATATATGGGAAGATGCCCTGGTTG
TGGACAATGGAATACACTTGTTGAAGAGATAGAACAAGTTGTATCATCTAGACGCCTTAATTATGCAAATGCGATTCAAA
CAGAGGTAACAAAACCAAGAAGGCTTATAGAAGTAGAAACAAAATCAGAAGCGCGTATTGAGACACAATTTCAAGAATTT
AATCGTGTATTAGGTGGAGGTATTGTTGATGGTTCCCTTGTTCTTATCGGAGGAGATCCTGGAATTGGGAAATCGACTTT
ACTGTTGCAAATATCATCACAATTAGCAAATTCTGCTTATGATGTTCTGTATATATCAGGAGAAGAATCCGCAAAACAAA
TCAAGCTTCGTGCAGATCGTTTACATGTAAATGGCCGGAATCTGTTCGTTGTTTCAGAAACAGATTTACAGCGCATTGCA
GCTCATATTGAAGAAATGAATCCCTCTTTTGTTGTAATTGATTCTATTCAAACCATTTATCTACCGGAAGTTACTTCAGC
ACCTGGAAGTGTGGCACAAGTACGTGAATGTACGGCGGAACTGATGAAACTTGCGAAAACGAAAGGAATCCCCATTTTTA
TTGTTGGGCATGTGACAAAAGAAGGTGCGATTGCAGGGCCACGTATGCTAGAACATATGGTAGATGCGGTTCTTTATTTT
GAAGGAGATCGTCATCATACATATCGTATTTTGCGAGCAGTAAAAAACCGATTTGGTTCTACAAATGAAATGGGTATTTT
TGAAATGAAAGAGCTGGGTCTTGCAGAAGTATTGAATCCTTCTGAAATTTTCCTAGAAGAAAGACCGGTCGGAGTTGCAG
GGTCTACAGTTGTTGCCTCAATGGAAGGAACAAGACCTGTTTTAGTAGAAATACAAGCATTAATCTCTCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCCACAGGAATTGATCATAATCGTGTATCTCTTATTATGGCAGTGTTGGAAAAGAGAGCCGG
ATTATTGTTGCAAAATCAAGATGCGTATTTGAAAGTGGCTGGAGGTTTGAAGTTAGATGAACCAGCGATTGACTTAGCGG
TTGCAATAAGCATTGCTTCAAGTTTTCGAGATAAATCTACGGCACCGACTGATGCGGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCTGCTAAATTAGGATTTCAACGTGCAATTATCCC
GCGGAAAAATTTAGGTGGATGGACAATTCCTGAGGGGATTGAAGTGGTTGGTGTTTCCAATTTAGGCGAGGCGCTGCGAT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(61-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

75.599

100

0.758
  radA Streptococcus pneumoniae Rx1

64.018

98.908

0.633
  radA Streptococcus pneumoniae D39

64.018

98.908

0.633
  radA Streptococcus pneumoniae R6

64.018

98.908

0.633
  radA Streptococcus pneumoniae TIGR4

64.018

98.908

0.633
  radA Streptococcus mitis SK321

64.018

98.908

0.633
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631