Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KZY06_RS21205 Genome accession   NZ_CP080146
Coordinates   4440375..4441052 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain PK8276     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4435375..4446052
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZY06_RS21190 (KZY06_21180) chiA 4436742..4439435 (+) 2694 WP_000773143.1 bifunctional chitinase/lysozyme -
  KZY06_RS21195 (KZY06_21185) bfd 4439604..4439798 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KZY06_RS21200 (KZY06_21190) bfr 4439870..4440346 (+) 477 WP_000675504.1 bacterioferritin -
  KZY06_RS21205 (KZY06_21195) pilD 4440375..4441052 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  KZY06_RS21210 (KZY06_21200) - 4441159..4442320 (+) 1162 Protein_4175 IS3-like element IS3 family transposase -
  KZY06_RS21215 (KZY06_21205) gspM 4442348..4442773 (-) 426 WP_086977277.1 type II secretion system protein GspM -
  KZY06_RS21220 (KZY06_21210) gspL 4442770..4443933 (-) 1164 WP_001115228.1 type II secretion system protein GspL -
  KZY06_RS21225 (KZY06_21215) gspK 4443948..4444931 (-) 984 WP_001058521.1 type II secretion system minor pseudopilin GspK -
  KZY06_RS21230 (KZY06_21220) gspJ 4444924..4445511 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  KZY06_RS21235 (KZY06_21225) gspI 4445504..4445881 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=517809 KZY06_RS21205 WP_000178161.1 4440375..4441052(-) (pilD) [Escherichia coli strain PK8276]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=517809 KZY06_RS21205 WP_000178161.1 4440375..4441052(-) (pilD) [Escherichia coli strain PK8276]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378