Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KZY05_RS18190 Genome accession   NZ_CP080139
Coordinates   3567175..3568671 (-) Length   498 a.a.
NCBI ID   WP_032202356.1    Uniprot ID   -
Organism   Escherichia coli strain PK8241     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3562175..3573671
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZY05_RS18165 (KZY05_18175) panM 3562397..3562780 (+) 384 WP_000778792.1 aspartate 1-decarboxylase autocleavage activator PanM -
  KZY05_RS18170 (KZY05_18180) livJ 3562980..3564083 (-) 1104 WP_001022007.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  KZY05_RS18175 (KZY05_18185) rpoH 3564354..3565208 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  KZY05_RS18180 (KZY05_18190) ftsX 3565453..3566511 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  KZY05_RS18185 (KZY05_18195) ftsE 3566504..3567172 (-) 669 WP_000617726.1 cell division ATP-binding protein FtsE -
  KZY05_RS18190 (KZY05_18200) pilA 3567175..3568671 (-) 1497 WP_032202356.1 signal recognition particle-docking protein FtsY Machinery gene
  KZY05_RS18195 (KZY05_18205) rsmD 3568821..3569417 (+) 597 WP_000743202.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  KZY05_RS18200 (KZY05_18210) yhhL 3569407..3569676 (+) 270 WP_001295207.1 DUF1145 family protein -
  KZY05_RS18205 (KZY05_18215) yhhM 3569679..3570038 (-) 360 WP_000042886.1 DUF2500 domain-containing protein -
  KZY05_RS18210 (KZY05_18220) yhhN 3570179..3570805 (+) 627 WP_000964724.1 lysoplasmalogenase -
  KZY05_RS18215 (KZY05_18225) zntA 3570879..3573077 (+) 2199 WP_000106560.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  KZY05_RS18220 (KZY05_18230) tusA 3573179..3573424 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54680.47 Da        Isoelectric Point: 4.1639

>NTDB_id=517698 KZY05_RS18190 WP_032202356.1 3567175..3568671(-) (pilA) [Escherichia coli strain PK8241]
MAKEKKRGFFSWLGFGQKEQTPEKDTEVQNEQPVVEEIVQAQEPAKASEHAVEEQPQAHTEAEAETFAAEVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=517698 KZY05_RS18190 WP_032202356.1 3567175..3568671(-) (pilA) [Escherichia coli strain PK8241]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGATACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGCGAAGGCTTCGGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAGGCCGAAGCGGAAACTTTTGCTGCCGAAGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTTTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCACGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGTTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378