Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   JC622_RS21175 Genome accession   NZ_CP066125
Coordinates   4517875..4519314 (-) Length   479 a.a.
NCBI ID   WP_005336331.1    Uniprot ID   A0A0T6N7F3
Organism   Aeromonas veronii strain 32286     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4512875..4524314
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JC622_RS21145 dagF 4513143..4513883 (+) 741 WP_181269563.1 2-dehydro-3-deoxy-phosphogluconate aldolase -
  JC622_RS21150 - 4513991..4515895 (+) 1905 WP_227095403.1 transcription antiterminator -
  JC622_RS21155 npr 4515913..4516185 (-) 273 WP_005336318.1 PTS phosphocarrier protein NPr -
  JC622_RS21160 rapZ 4516206..4517072 (-) 867 WP_005336320.1 RNase adapter RapZ -
  JC622_RS21165 ptsN 4517118..4517564 (-) 447 WP_005336322.1 PTS IIA-like nitrogen regulatory protein PtsN -
  JC622_RS21170 hpf 4517567..4517854 (-) 288 WP_005336323.1 ribosome hibernation promoting factor -
  JC622_RS21175 rpoN/rpoN1 4517875..4519314 (-) 1440 WP_005336331.1 RNA polymerase factor sigma-54 Machinery gene
  JC622_RS21180 lptB 4519380..4520105 (-) 726 WP_005336333.1 LPS export ABC transporter ATP-binding protein -
  JC622_RS21185 lptA 4520109..4520636 (-) 528 WP_040068817.1 lipopolysaccharide transport periplasmic protein LptA -
  JC622_RS21190 lptC 4520623..4521183 (-) 561 WP_043821874.1 LPS export ABC transporter periplasmic protein LptC -
  JC622_RS21195 kdsC 4521180..4521734 (-) 555 WP_227095404.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  JC622_RS21200 - 4521734..4522729 (-) 996 WP_227095405.1 KpsF/GutQ family sugar-phosphate isomerase -
  JC622_RS21205 mlaF 4522937..4523737 (+) 801 WP_227095406.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53701.64 Da        Isoelectric Point: 4.3442

>NTDB_id=517625 JC622_RS21175 WP_005336331.1 4517875..4519314(-) (rpoN/rpoN1) [Aeromonas veronii strain 32286]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALENNPLLEQEESEAQETSPEAPVDASQLDSSDAMAQEQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVALED
IQAAVTSDDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLDQYDPQTPWLNEAKEVILEHMELLGNRDYRTLARK
TKLKESDLKEVLDLIQLLEPRPGNGIIQSDSQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNETYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=517625 JC622_RS21175 WP_005336331.1 4517875..4519314(-) (rpoN/rpoN1) [Aeromonas veronii strain 32286]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAAAACAACCCTCTGCTGGAGCAGGAAGAGAGCGAGG
CGCAAGAGACCAGTCCGGAAGCGCCGGTCGATGCCAGCCAGCTGGATTCCAGCGATGCCATGGCCCAGGAGCAGATGCCG
GACGAGTTGCCGGTGGATACCACCTGGGACGAGGTTTACTCTGCCGGCACCGCCCAGAGCGCGGGCCCCATCCACGATGG
GGAAGATAGCGTCTATCAGGGAGAGACCACCGAAAACCTGCAGGATTATCTGTTGTGGCAGATGCGACTCACACCGTTCA
GCGATGTGGATGCCGCCATCGCGCTCGCCATCATCGATGCCATCGACGAATCCGGTTATCTGACCGTTGCGCTGGAGGAT
ATCCAGGCTGCAGTCACCAGCGATGACGTAGAGGTGGAGCTCGATGAGGTCGAGGCGGTACTCAAGCGGGTGCAGCACTT
CGATCCAGTCGGCATTGCTGCCCGTTCTGTGCAGGAGTGTTTGCTGATCCAGCTGGATCAGTATGACCCGCAGACCCCCT
GGCTCAACGAGGCCAAGGAGGTGATCCTCGAGCACATGGAGCTGCTCGGCAACCGGGATTACCGCACCCTCGCCCGCAAG
ACAAAACTCAAGGAGAGCGACCTCAAGGAGGTGCTGGATCTGATCCAGCTGCTGGAGCCGCGTCCCGGCAACGGCATCAT
CCAGAGCGACTCCCAGTACGTGATCCCCGACGTCTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTCGAGCTCAATCCCG
ACTCCGCCCCCCGCATTCGGGTCAACGAGACCTATGCCGCCATGGCCCGCAACGCCCGCAGCAGCACCGACACCCAGTTT
ATCCGCTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGCCTGGAAAGTCGCAATGACACCCTGCTGAAAGTGGCCAG
TTGCATTGTCGAGCAGCAACAGGGCTTTTTCGAGTATGGTGAAGAGGCGATGAAGCCGATGGTGCTCAATGACATCGCCG
AAGCGGTAGAGATGCATGAGTCGACCATCTCCCGGGTGACGACCCAGAAGTACATGCATACCCCCCGTGGTATCTTCGAA
TTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCAGAGAATCCTGCCAAGCCGTTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCTAAGTATCGCGAATCCTTGTTTATCCCACCTTCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T6N7F3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.988

100

0.491