Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KZY03_RS18585 Genome accession   NZ_CP080126
Coordinates   3825389..3825829 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain PK8568     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3820389..3830829
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZY03_RS18565 (KZY03_18565) aroP 3821387..3822760 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  KZY03_RS18570 (KZY03_18570) ampE 3822803..3823657 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  KZY03_RS18575 (KZY03_18575) ampD 3823654..3824205 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KZY03_RS18580 (KZY03_18580) nadC 3824293..3825186 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KZY03_RS18585 (KZY03_18585) pilA 3825389..3825829 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  KZY03_RS18590 (KZY03_18590) pilB 3825839..3827224 (+) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  KZY03_RS18595 (KZY03_18595) hofC 3827214..3828416 (+) 1203 WP_000157234.1 protein transport protein HofC -
  KZY03_RS18600 (KZY03_18600) guaC 3828451..3829494 (-) 1044 WP_001217338.1 GMP reductase -
  KZY03_RS18605 (KZY03_18605) - 3829650..3829694 (-) 45 WP_120795372.1 protein YacM -
  KZY03_RS18610 (KZY03_18610) coaE 3829719..3830339 (+) 621 WP_001269531.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=517546 KZY03_RS18585 WP_000360904.1 3825389..3825829(+) (pilA) [Escherichia coli strain PK8568]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=517546 KZY03_RS18585 WP_000360904.1 3825389..3825829(+) (pilA) [Escherichia coli strain PK8568]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363